Multi-scale characterization of the spatio-temporal interplay between elemental composition, mineral deposition and remodelling in bone fracture healing

Dejea, Hector; Raina, Deepak; Barreto, Isabella Silva; Sharma, K. K.; Liu, Yang; Sánchez, Darío Ferreira; Johansson, Ulf; Isaksson, Hanna

doi:10.1016/j.actbio.2023.06.031

Cited by 3 publications

(3 citation statements)

References 53 publications

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“…From the literature, Fe can be linked to procollagen enzymes required for precursors in collagen . Furthermore, Fe was shown to accumulate at mineralization fronts, , inside osteoblasts, and along edges of trabeculae structures . However, whether HO deposits in healing rat Achilles tendons share similar elemental characteristics has not been reported previously, to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 85%

“…From bone formation, it is known that the presence of proteins, such as matrix metalloproteinase (MMP) −9 and −13, play a pivotal role in remodeling the extracellular matrix with zinc (Zn) as a known cofactor. , The presence of Zn at mineralization boundaries was shown in our previous studies using rodent models in long bone fracture healing and embryonic long bone development . The study on bone fracture healing also reported the presence of Iron (Fe) ahead of Zn at the mineralization front; however, the role that Fe plays at the mineralization front needs to be investigated further. From the literature, Fe can be linked to procollagen enzymes required for precursors in collagen .…”

Section: Introductionmentioning

confidence: 99%

“…Further hierarchical probing at the micrometer and nanometer scales can be achieved through synchrotron-based techniques . Small- and wide-angle X-ray scattering (SAXS/WAXS) techniques have been utilized extensively to investigate HA crystallite dimensions in bone. ,,− X-ray fluorescence (XRF) has been widely used to determine the elemental composition of mineralized tissues to elucidate mineralization pathways. ,,,, …”

Section: Introductionmentioning

confidence: 99%

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Elemental and Structural Characterization of Heterotopic Ossification during Achilles Tendon Healing Provides New Insights on the Formation Process

Sharma,

Silva Barreto,

Dejea

et al. 2024

ACS Biomater. Sci. Eng.

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Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at 3, 6, 12, 16, and 20 weeks post transection. The microscale tomography and scanning electron microscopy results indicate increased mineral density and Ca/P ratio in the maturing HO deposits (12 and 20 weeks), when compared to the early time points (3 weeks). Visually, the mature HO deposits present microstructures similar to calcaneal bone. Through synchrotron-based X-ray scattering and fluorescence, the hydroxyapatite (HA) crystallites are shorter along the c-axis and become larger in the ab-plane with increasing healing time, while the HA crystal thickness remains within the reference values for bone. At the mineralization boundary, the overlap between high levels of calcium and prominent crystallite formation was outlined by the presence of zinc and iron. In the mature HO deposits, the calcium content was highest, and zinc was more present internally, which could be indicative of HO deposit remodeling. This study emphasizes the structural and elemental similarities between the calcaneal bone and HO deposits.

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Section: Introductionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Elemental and Structural Characterization of Heterotopic Ossification during Achilles Tendon Healing Provides New Insights on the Formation Process

Sharma,

Silva Barreto,

Dejea

et al. 2024

ACS Biomater. Sci. Eng.

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Crossing length scales: X-ray approaches to studying the structure of biological materials

Grünewald,

Liebi,

Birkedal

2024

IUCrJ

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Biological materials have outstanding properties. With ease, challenging mechanical, optical or electrical properties are realised from comparatively `humble' building blocks. The key strategy to realise these properties is through extensive hierarchical structuring of the material from the millimetre to the nanometre scale in 3D. Though hierarchical structuring in biological materials has long been recognized, the 3D characterization of such structures remains a challenge. To understand the behaviour of materials, multimodal and multi-scale characterization approaches are needed. In this review, we outline current X-ray analysis approaches using the structures of bone and shells as examples. We show how recent advances have aided our understanding of hierarchical structures and their functions, and how these could be exploited for future research directions. We also discuss current roadblocks including radiation damage, data quantity and sample preparation, as well as strategies to address them.

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Sheep Bone Ultrastructure Analyses Reveal Differences in Bone Maturation around Mg-Based and Ti Implants

Iskhakova,

Wieland,

Marek

et al. 2024

JFB

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Magnesium alloys are some of the most convenient biodegradable materials for bone fracture treatment due to their tailorable degradation rate, biocompatibility, and mechanical properties resembling those of bone. Despite the fact that magnesium-based implants and ZX00 (Mg-0.45Zn-0.45Ca in wt.%), in particular, have been shown to have suitable degradation rates and good osseointegration, knowledge gaps remain in our understanding of the impact of their degradation properties on the bone’s ultrastructure. Bone is a hierarchically structured material, where not only the microstructure but also the ultrastructure are important as properties like the local mechanical response are determined by it. This study presents the first comparative analysis of bone ultrastructure parameters with high spatial resolution around ZX00 and Ti implants after 6, 12, and 24 weeks of healing. The mineralization was investigated, revealing a significant decrease in the lattice spacing of the (002) Bragg’s peak closer to the ZX00 implant in comparison to Ti, while no significant difference in the crystallite size was observed. The hydroxyapatite platelet thickness and osteon density demonstrated a decrease closer to the ZX00 implant interface. Correlative indentation and strain maps obtained by scanning X-ray diffraction measurements revealed a higher stiffness and faster mechanical adaptation of the bone surrounding Ti implants as compared to the ZX00 ones. Thus, the results suggest the incorporation of Mg2+ ions into the bone ultrastructure, as well as a lower degree of remodeling and stiffness of the bone in the presence of ZX00 implants than Ti.

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Multi-scale characterization of the spatio-temporal interplay between elemental composition, mineral deposition and remodelling in bone fracture healing

Cited by 3 publications

References 53 publications

Elemental and Structural Characterization of Heterotopic Ossification during Achilles Tendon Healing Provides New Insights on the Formation Process

Elemental and Structural Characterization of Heterotopic Ossification during Achilles Tendon Healing Provides New Insights on the Formation Process

Crossing length scales: X-ray approaches to studying the structure of biological materials

Sheep Bone Ultrastructure Analyses Reveal Differences in Bone Maturation around Mg-Based and Ti Implants

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