Multi-responsive chitosan-based hydrogels for controlled release of vincristine

Abstract

As medical research progresses, the derivation and development of biological materials such as hydrogels have steadily gained more interest. The biocompatibility and non-toxicity of chitosan make chitosan hydrogels potential carriers for drug delivery. This work aims to develop two multi-reactive, safe, and highly swellable bio-hydrogels consisting of chitosan-graft-glycerol (CS-g-gly) and carboxymethyl chitosan-graft-glycerol (CMCS-g-gly), for sustained and controlled drug release, improved bioavailability along with entrapment in nanocarriers, which reduces side effects of vincristine sulphate. CS-g-gly and CMCS-g-gly are successfully prepared and fully characterized using analytical techniques. Under various conditions, the prepared hydrogels exhibit a high swelling ratio. Vincristine-loaded CS-g-gly (VCR/CS-g-gly), and CMCS-g-gly (VCR/CMCS-g-gly) show high encapsulation efficiency between 72.28-89.97%, and 56.97-71.91%, respectively. VCR/CS-g-gly show a sustained release behavior, and the maximum release of VCR from hydrogels reached 82% after 120 h of incubation. MCF-7 (breast cancer cell line) and MCF-10 (normal breast cell line) are evaluated for cell viability and apoptosis induction. The in-vitro anti-tumor efficacy is investigated using flow cytometry. The tetrazolium-based MTT assay of hydrogels shows no evidence of significant cytotoxicity in MCF-7 and MCF-10 cells. According to these findings, these hydrogels can effectively deliver drugs to MCF-7 and other breast cancer cells.

Chitosan-Based Smart Biomaterials for Biomedical Applications: Progress and Perspectives

“…In addition to being in charge of mucosal adherence, chitosan also possesses smart stimuli-responsive properties due to its primary amino groups. Such properties promote chitosan to be an advanced biopolymer for wide use in the formulation of many smart polymeric DDSs. , …”

“…Such properties promote chitosan to be an advanced biopolymer for wide use in the formulation of many smart polymeric DDSs. 50,51 2.2.1. pH-Responsive of Chitosan-Based Smart Drug Carrier. pH plays a key role in maintaining cell and tissue homeostasis.…”

Chitosan-Grafted-Poly(N-vinylcaprolactam)-Decorated Fe3O4@SiO2 Core–Shell Nanoformulation as an Efficient Drug Delivery System for Poorly Soluble Drugs

“…Drug delivery systems (DDSs) play a crucial role in treating a variety of diseases by facilitating the efficient delivery of therapeutics to specific target sites while minimizing potential side effects. , Poorly soluble drugs pose significant drug development and delivery challenges, impacting their therapeutic effectiveness and patient compliance. Limited aqueous solubility often leads to low bioavailability and variable therapeutic outcomes .…”

“…Among the natural polymers, chitosan (CS) plays a pivotal role in advancing DDSs. This biocompatible, biodegradable, and nontoxic polyelectrolyte has garnered significant attention in biomedicine and drug delivery . CS exhibits remarkable characteristics that make it an attractive choice for DDS.…”

“…This biocompatible, biodegradable, and nontoxic polyelectrolyte has garnered significant attention in biomedicine and drug delivery. 1 CS exhibits remarkable characteristics that make it an attractive choice for DDS. It possesses excellent chelation and adsorption capabilities, allowing it to bind and carry drugs efficiently.…”