Multi-Product Microalgae Biorefineries: From Concept Towards Reality

Lam, G.P. ‘t; Vermuë, M.H.; Eppink, M.H.M.; Wijffels, René H.; Berg, C. van den

doi:10.1016/j.tibtech.2017.10.011

Cited by 200 publications

(65 citation statements)

References 75 publications

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“…Although microalgal cells contain several valuable biomolecules, the currently applied separation processes valorize only one specific product (e.g. astaxanthin, phycobiliproteins) . The development of efficient, mild and scalable methods/processes, capable of fractionating different microalgal biomolecules, is of particular importance .…”

Section: Introductionmentioning

confidence: 99%

Selective and mild fractionation of microalgal proteins and pigments using aqueous two‐phase systems

Ruiz

Emmery

Wijffels

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUNDMicroalgal biomass is generally used to produce a single product instead of valorizing all of the cellular components. The biomass production and downstream processes are too expensive if only one product is valorized. A new approach was proposed for the simultaneous and selective partitioning of pigments and proteins from disrupted Neochloris oleoabundans cultivated under saline and freshwater conditions.RESULTSAn aqueous two‐phase system composed of polyethylene glycol and cholinium dihydrogen phosphate selectively separated microalgal pigments from microalgal proteins. 97.3 ± 1.0% of lutein and 51.6 ± 2.3% of chlorophyll were recovered in the polymer‐rich phase. Simultaneously, up to 92.2 ± 2.0% of proteins were recovered in a third phase (interface) in between the aqueous phases (interface). The recovered proteins, including Rubisco with a molecular weight of ∼560 kDa, seem to be intact and pigments did not suffer degradation, demonstrating the mildness of this system for fractionating microalgal biomolecules.CONCLUSIONThe ability of aqueous two‐phase systems (ATPSs) to simultaneously and efficiently fractionate different biomolecules in a mild manner from disrupted microalgae is demonstrated. This is an important step towards the development of a multiproduct microalgae biorefinery. © 2018 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Selective and mild fractionation of microalgal proteins and pigments using aqueous two‐phase systems

Ruiz

Emmery

Wijffels

et al. 2018

J of Chemical Tech & Biotech

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“…The usage of MNPs in food industry presents a very extensive application. They may be used as pigments, gelling agents, preservatives, stabilizers, and ingredients . With the rise of functional foods, MNPs have also been described as complementary ingredients, dietary supplements, prebiotics, and nutraceuticals .…”

Section: Applications Of Mnps In Biotechnology Bioengineering and Pmentioning

confidence: 99%

“…Currently, several types of products are extracted from marine sources such as pigments, proteins, excreted compounds, or metabolites, to cite a few . To aggregate this information in a clear and useful way several databases are already widely used, not only regarding genomics and proteomics of marine organisms, like the MAR databases, but also marine compounds, for example, the paid‐access MarinLit .…”

Section: Introductionmentioning

confidence: 99%

“…The availability of free‐access chemical databases of MNPs should prompt and accelerate putative applications for a myriad of marine origin compounds. Data could be applied not only to the discovery of new bioactive compounds but also to new materials and high‐value molecules, e.g., pigments from microalgae . An important issue that could be attenuated with more accessible marine product databases is MNP bioprospecting and dereplication .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Free Marine Natural Products Databases for Biotechnology and Bioengineering

Barbosa

Roque

2019

Biotechnology Journal

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Marine organisms and micro‐organisms are a source of natural compounds with unique chemical features. These chemical properties are useful for the discovery of new functions and applications of marine natural products (MNPs). To extensively exploit the potential implementations of MNPs, they are gathered in chemical databases that allow their study and screening for applications of biotechnological interest. However, the classification of MNPs is currently poor in generic chemical databases. The present availability of free‐access‐focused MNP databases is scarce and the molecular diversity of these databases is still very low when compared to the paid‐access ones. In this review paper, the current scenario of free‐access MNP databases is presented as well as the hindrances involved in their development, mainly compound dereplication. Examples and opportunities for using freely accessible MNP databases in several important areas of biotechnology are also assessed. The scope of this paper is, as well, to notify the latent potential of these information sources for the discovery and development of new MNPs in biotechnology, and push future efforts to develop a public domain MNP database freely available for the scientific community.

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“…It has been repeatedly concluded that cultivation in photobioreactors is more costly than open raceway ponds in terms of capital investment, but when downstream processing costs are considered, the higher cell densities achievable in photobioreactors are more economically advantageous (Davis et al 2011). Harvesting and dewatering are time and energy-intensive steps and can account for anywhere between 10 and 50% of total operating costs due to the dilute nature of algae suspensions and large process volume (Barsanti and Gualtieri 2018;t'Lam et al 2018;Ríos et al 2013). Operations including microfiltration and centrifugation have been found to cost almost twice that of pH or gravity sedimentation (Ríos et al 2013).…”

Section: Economic Considerationsmentioning

confidence: 99%

Green microalgae biomolecule separations and recovery

Dixon

Wilken

2018

Bioresour. Bioprocess.

111

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Microalgae biomass has garnered significant attention as a renewable energy feedstock and alternative to petroleumbased fuels. The diverse metabolism of green microalgae species additionally provides opportunities for recovery of products for feed, food, nutraceutical, cosmetic, and biopharmaceutical industries. Recently, the concept of using microalgae as part of a biorefinery model has been adopted in place of refinery methods focused on recovering one target product. This has led to producers exploring co-production of high value and high volume products in an effort to improve process economics. With numerous potential products and applications, the biomass source or specific strain must be carefully selected to accumulate extractable levels of the target molecule(s). It is additionally imperative to understand the morphology and metabolism of the selected strain to cost-effectively manage all stages of commercial production. This review will focus specifically on microalgae in the division of Chlorophyta, or green algae and their extracellular matrices (ECM), potential for commercial products, and finally describe a holistic approach for biomolecule extraction and recovery. Additionally, cell disruption and fractionation methods for recovery of biomolecules for commercial products are highlighted along with an alternative method, aqueous enzymatic processing for multiple biomolecule extraction and recovery from green microalgae. An emphasis is placed on connecting the morphological characteristics of microalgae ECM or organelle membranes to implications on separation and purification technologies.

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Multi-Product Microalgae Biorefineries: From Concept Towards Reality

Cited by 200 publications

References 75 publications

Selective and mild fractionation of microalgal proteins and pigments using aqueous two‐phase systems

Selective and mild fractionation of microalgal proteins and pigments using aqueous two‐phase systems

Free Marine Natural Products Databases for Biotechnology and Bioengineering

Green microalgae biomolecule separations and recovery

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