Multi-Probiotic Lactobacillus Supplementation Improves Liver Function and Reduces Cholesterol Levels in Jeju Native Pigs

Kim, Dahye; Min, Yunhui; Yang, Jun; Heo, Yunji; Kim, Mangeun; Hur, Chang-Gi; Lee, Sangchul; Lee, Hak-Kyo; Song, Ki-Duk; Heo, Jae-Young; Son, Young‐Ok; Lee, Dong‐Sun

doi:10.3390/ani11082309

Cited by 6 publications

(4 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kim al. [45] found that cholesterol concentration was significantly decreased in the pigs fed with Lactobacillus-based probiotic. From this, it may be inferred that probiotic supplementation in weaning piglets improves the lipid profile by decreasing the TC and LDL concentration in serum and by increasing the HDLc concentration.…”

Section: Discussionmentioning

confidence: 93%

Effect of a Multi-Strain Probiotic on Growth Performance, Lipid Profile, Antioxidant Status and Immune Response in Piglets at Weaning

Sarkar,

Mondal,

Bhattacharya

et al. 2023

Preprint

View full text Add to dashboard Cite

The study investigated the effect of a multi-strain probiotic compound containing Bacillus coagulans, Enterococcus faecalis, Clostridium butyricum and Bacillus mesentericus as in-feed zinc oxide (ZnO) alterna-tive on growth performance, diarrhea incidence, antioxidant status, lipid profile, stress and immunity in weaned piglets. A total of 72 piglets were randomly divided into 3 groups with four replicates of six piglets each. The details of the groups were as follows; (i) weaned control group (WC) received basal diet, (ii) weaned probiotic group (WB) received basal diet and probiotics and (iii) positive control (PC) group received basal diet with 2500 mg/kg ZnO. The experiment lasted for 28 days. Probiotic supple-mentation improved growth performance and reduced diarrhea rate. Probiotics supplementation im-proved lipid profile; significantly lower levels of total cholesterol and low-density lipoprotein cholesterol and higher level of high-density lipoprotein cholesterol in WB group as compared to those of the control group (WC) were recorded. Probiotic supplementation stimulated antioxidant defense system by in-creasing total antioxidant capacity and decreasing lipid peroxidation. Probiotic supplementation down-regulated the stress biomarkers like serum cortisol and serum heat shock proteins. WB group showed higher serum levels of IgG and IgM throughout the study period and higher IgA at day 28 as compared to WC. These data suggest that supplementation of the probiotic minimizes the weaning stress, thereby improves the growth performance, lipid profile, antioxidant status and systemic as well as mucosal immunity. Therefore, the multi-strain probiotic compound may be used to replace ZnO in weaned piglets.

show abstract

Section: Discussionmentioning

confidence: 93%

Effect of a Multi-Strain Probiotic on Growth Performance, Lipid Profile, Antioxidant Status and Immune Response in Piglets at Weaning

Sarkar,

Mondal,

Bhattacharya

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Yu et al [49] found that selenium in combination with complex probiotics (Lactobacillus acidophilus, Lactobacillus pentose, and B. subtilis) tended to reduce TC, VLDL, and TG and increase HDLc concentrations in the blood. Kim et al [50] reported that the cholesterol concentration was significantly de-creased in pigs fed with Lactobacillus-based probiotic formulations. From this, it may be inferred that probiotic supplementation in weaning piglets can improve the lipid profile by decreasing the TC and LDL concentrations and increasing the HDLc concentration in serum.…”

Section: Discussionmentioning

confidence: 99%

Effect of a Multi-Strain Probiotic on Growth Performance, Lipid Panel, Antioxidant Profile, and Immune Response in Andaman Local Piglets at Weaning

Sarkar,

Mondal,

Bhattacharya

et al. 2023

Fermentation

View full text Add to dashboard Cite

This study aimed to investigate the role of a multi-strain probiotic compound containing Bacillus mesentericus, Bacillus coagulans, Enterococcus faecalis, and Clostridium butyricum as an in-feed zinc oxide (ZnO) alternative in growth performance, diarrhea incidence, antioxidant profile, lipid panel, stress, and immunity in piglets at weaning. Seventy-two piglets weaned at 27 ± 1 day were divided randomly into three groups with four replicates of six piglets each: (i) a negative control group (WC) fed only a basal diet, (ii) a probiotic group (WB) fed a basal diet with the current probiotic formulation, and (iii) a positive control (PC) group fed a basal diet with 2500 mg/kg ZnO. The experiment was conducted for 28 days. Probiotic supplementation showed a positive effect on growth performance and reduced the diarrhea rate. The mean body weight of the piglets in the WB and PC groups was significantly higher than that of piglets in the WC group (14.88 ± 0.12, 14.97 ± 0.13 vs. 13.80 ± 0.06 kg; p ≤ 0.001). The addition of probiotic to the diet improved the lipid panel; the WB group showed a significantly higher level of high-density lipoprotein cholesterol (mg/dL) (32.67 ± 0.85 in WB vs. 12.48 ± 0.76 in WC; p ≤ 0.001) and lower levels of total cholesterol (mg/dL) (59.78 ± 1.97 in WB vs. 119.11 ± 2.12 in WC; p ≤ 0.001) and low-density lipoprotein cholesterol (mg/dL) (17.90 ± 1.12 in WB vs. 69.10 ± 3.37 in WC; p ≤ 0.001) compared with the negative control group. Moreover, probiotic supplementation enhanced the antioxidant defense system and provided protection from oxidative damage by increasing the concentrations of serum catalase, glutathione-S-transferase, and superoxide dismutase and by decreasing the concentrations of serum malonyldialdehyde and total nitric oxide. Heat shock proteins and other stress markers, such as serum cortisol, were reduced in the probiotic-fed group. The probiotic group also displayed higher levels of serum IgG and IgM at all time points and higher IgA on day 28 compared with the negative control group. Altogether, these results indicate that feeding with the currently used multi-strain probiotic formulation minimizes weaning stress, thereby improving the growth performance, antioxidant profile, lipid panel, and systemic and mucosal immunity. Therefore, multi-strain probiotic compounds may be used to replace ZnO in weaned piglets.

show abstract

“…Although it is not fully understood how immune activation or tolerance is achieved in response to the probiotic bacteria, interactions between MAMPs and pattern recognition receptors (PRRs) of the host intestinal cells are involved (Liao and Nyachoti 2017 ). One such mechanism by which Lactobacillus -based probiotics suppress inflammation has been suggested to be initiated by TLR2 (Peng et al 2022 ; Wang et al 2019a ), TLR4 (Peng et al 2022 ; Tang et al 2021 ; Yi et al 2018 ), or NOD1 activation (Kim et al 2021 ) through NF-κB or MAPK signaling pathways in the jejunum and ileum of pigs. Thereafter, the recognition of probiotic antigens leads to regulations of cell-mediated immune responses in the porcine intestine.…”

Section: Alternatives To Antibiotics In Pig Production: Modulating Th...mentioning

confidence: 99%

Alternatives to antibiotics in pig production: looking through the lens of immunophysiology

Liu,

Zhu,

Zhu

et al. 2024

Stress Biology

View full text Add to dashboard Cite

In the livestock production system, the evolution of porcine gut microecology is consistent with the idea of “The Hygiene Hypothesis” in humans. I.e., improved hygiene conditions, reduced exposure to environmental microorganisms in early life, and frequent use of antimicrobial drugs drive immune dysregulation. Meanwhile, the overuse of antibiotics as feed additives for infectious disease prevention and animal growth induces antimicrobial resistance genes in pathogens and spreads related environmental pollutants. It justifies our attempt to review alternatives to antibiotics that can support optimal growth and improve the immunophysiological state of pigs. In the current review, we first described porcine mucosal immunity, followed by discussions of gut microbiota dynamics during the critical weaning period and the impacts brought by antibiotics usage. Evidence of in-feed additives with immuno-modulatory properties highlighting probiotics, prebiotics, and phytobiotics and their cellular and molecular networking are summarized and reviewed. It may provide insights into the immune regulatory mechanisms of antibiotic alternatives and open new avenues for health management in pig production.

show abstract

Multi-Probiotic Lactobacillus Supplementation Improves Liver Function and Reduces Cholesterol Levels in Jeju Native Pigs

Cited by 6 publications

References 45 publications

Effect of a Multi-Strain Probiotic on Growth Performance, Lipid Profile, Antioxidant Status and Immune Response in Piglets at Weaning

Effect of a Multi-Strain Probiotic on Growth Performance, Lipid Profile, Antioxidant Status and Immune Response in Piglets at Weaning

Effect of a Multi-Strain Probiotic on Growth Performance, Lipid Panel, Antioxidant Profile, and Immune Response in Andaman Local Piglets at Weaning

Alternatives to antibiotics in pig production: looking through the lens of immunophysiology

Contact Info

Product

Resources

About