Multi‐potent differentiation of human purified muscle‐derived cells: potential for tissue regeneration

Abstract

OBJECTIVETo investigate whether CD34+ purified human muscle‐derived cells (hMDCs) are capable of multiple lineage differentiation.MATERIALS AND METHODSThe hMDCs were isolated from human skeletal muscle and purified using a CD34+ cell selection system (Dynal Biotech, Oslo, Norway). Adherent populations of cells were expanded in culture and cell differentiation was induced using different kinds of growth factors and different differentiation‐conditional media. The immunohistochemical properties of CD34+ hMDCs were examined after varying periods in culture. Reverse transcription‐polymerase chain reaction (RT‐PCR) and Western blotting were used to investigate the gene expression of the undifferentiated and differentiated hMDCs.RESULTSUsing special differentiation conditions the CD34+ hMDCs could be differentiated into myogenic cells, adipocytes, osteocytes and chondrocytes. The differentiation was confirmed by immunohistochemistry. RT‐PCR and Western blotting showed multiple‐lineage gene‐level expression in the different cultivation periods of the differentiated cells.CONCLUSIONSWe confirmed the multi‐lineage capacity of a population of stem cells, termed CD34+ hMDCs. Our findings showed that CD34+ hMDCs are capable of multiple mesodermal‐lineage differentiation, as shown by the expression of several lineage‐specific genes. They can be differentiated toward the myogenic, osteogenic, adipogenic and chondrogenic lineages. These cells might have potential for use in tissue regeneration.

Muscle-derived stem cells: isolation, characterization, differentiation, and application in cell and gene therapy

“…In vitro, when cultivated in basic medium, MDSCs can spontaneously differentiate into myotubes (Lu et al 2009;Tamaki et al 2002), and when stimulated with inductive factors, MDSCs display a vigorous capacity for differentiation into tissue types from ectodermal and mesodermal cell types, including neural, hematopoietic, osteogenic (Bosch et al 2000;Levy et al 2001), adipogenic, chondrogenic ), and endothelial cell types (Alessandri et al 2004;Arsic et al 2008;Baek et al 2009;Deasy and Huard 2002;Deasy et al 2001;RomeroRamos et al 2002;Schultz and Lucas 2006;Vourc'h et al 2004). However, are MDSCs able to differentiate into epithelial cells, hepatocytes, or pancreatic cells?…”

Recent Progress in Interfacial Tissue Engineering Approaches for Osteochondral Defects

“…Figure 3d shows the deposition of hydroxyapatite, a crystalline compound found in bone, within the deposited ECM cell sheet after a 2-week culture period. Other stem cell sources have been investigated with regards to osteogenic and chondrogenic differentiation potential including adipose tissue, 35,41,46,82,96,112 periosteum, 14,17,18,94 skeletal muscle, 3,61,66,114 and umbilical cord 22,32,69,100 with most scaffold-free investigations focused on BMSCs and mature chondrocytes. 80 The primary goal of scaffold-free tissue engineering is the elimination of exogenous materials for better host integration and tissue regeneration.…”

Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model

“…Compared to the above-mentioned cell sources, myoblasts represent a more promising source for meniscal engineering, as they are relatively abundant and easily accessible with minimal donor site morbidity. At the same time, myoblasts further promote the development of tissue engineering, as they have a higher cell yield and more rapid proliferative ability during in vitro expansion (11).…”