Multi-platform genotoxicity analysis of silver nanoparticles in the model cell line CHO-K1

Jiang, Xiumin; Foldbjerg, Rasmus; Miclăuş, Teodora; Wang, Liming; Singh, Rajinder; Hayashi, Yuya; Sutherland, Duncan S.; Chen, Chunying; Autrup, Herman; Beer, Christiane

doi:10.1016/j.toxlet.2013.07.011

Cited by 110 publications

(97 citation statements)

References 59 publications

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“…The amount of RONS was much higher after exposure to AgEO-than to AgCitrate-NPs, which again confirms the previous results from this study. The observed absence of Ag-NPs from the mitochondria of the cells implies that the increase in RONS may rather be due to the release of Ag + than to the Ag-NPs themselves, which was found in other studies as well [29,30] and has been termed the "Trojan Horse Theory" [30]. Also, the citrate-coating may reduce the release of Ag + from the Ag-NPs which might offer an explanation for the decreased potential of the AgCitate-NPs to generate RONS.…”

Section: Inflammatory Reaction In Pbcecsupporting

confidence: 67%

“…Hence, the potential of AgEO-and AgCitrate-NP to trigger oxidative stress and subsequent DNA damage was analyzed in PBCEC and PCPEC. The potential of Ag-NPs to trigger oxidative stress and DNA damage has already been shown in many studies [5,20,29,34]. However, this has not yet been elucidated in barrier-forming cells of the CNS.…”

Section: Inflammatory Reaction In Pbcecmentioning

confidence: 99%

“…Thereby, the observed clustering may hinder the NPs from entering the nucleus. However, adverse effects can also be caused by Ag-NPs within the cytoplasma, for example through the release of Ag + ions [29,30]. A possible explanation for the lesser toxicity of AgCitrate-NPs compared to AgEO-NPs may also be a reduced release of Ag + ions.…”

Section: Inflammatory Reaction In Pbcecmentioning

confidence: 99%

“…In addition, DNA damage was detected in the Comet-Assay and was already evident at subtoxic concentrations which did not even elicit RONS. Xiang et al [29] evaluated the potential of BSA-coated AgNPs (15 nm) to cause genotoxicity in CHO-K1. While DNA adducts were already detected at small, non-toxic concentrations (1 µg/mL) the formation of 8-oxo-guanosin and micronuclei formation were only found at toxic concentrations that also elicited RONS.…”

Section: Inflammatory Reaction In Pbcecmentioning

confidence: 99%

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The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro

Cramer¹,

Tacke²,

Bornhorst³

et al. 2014

J Nanomed Nanotechnol

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The use of silver nanoparticles in medical and consumer products such as wound dressings, clothing and cosmetic has increased significantly in recent years. Still, the influence of these particles on our health and especially on our brain, has not been examined adequately up to now. We studied the influence of AgEO-(Ethylene Oxide) and AgCitrate-Nanoparticles (NPs) on the protective barriers of the brain, namely the blood-brain barrier (BBB) and the blood-cerebrospinal fluid (blood-CSF) barrier in vitro. The NPs toxicity was evaluated by examining changes in membrane integrity, cell morphology, barrier properties, oxidative stress and inflammatory reactions. AgNPs decreased cell viability, disturbed barrier integrity and tight junctions and triggered oxidative stress and DNA strand breaks. However, all mentioned effects were, at least partly, suppressed by a Citrate-coating and were most pronounced in the cells of the BBB as compared to the epithelial cells representing the blood-CSF barrier. AgEO-but not AgCitrate-NPs also triggered an inflammatory reaction in porcine brain capillary endothelial cells (PBCEC), which represent the BBB. Our data indicate that AgNPs may cause adverse effects within the barriers of the brain, but their toxicity can be reduced by choosing an appropriate coating material.

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Section: Inflammatory Reaction In Pbcecsupporting

confidence: 67%

Section: Inflammatory Reaction In Pbcecmentioning

confidence: 99%

Section: Inflammatory Reaction In Pbcecmentioning

confidence: 99%

Section: Inflammatory Reaction In Pbcecmentioning

confidence: 99%

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The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro

Cramer¹,

Tacke²,

Bornhorst³

et al. 2014

J Nanomed Nanotechnol

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“…According to random slices of TXM phase contrast images ( Figure S4, Supporting Information), we observed that at 24 h uptake most of AgNPs were located in the cytoplasm, in accordance with other reports (based on TEM) that AgNPs are mainly located in the endosomes and the lysosomes. 13,19,22 A comparison of this spatial distribution of silver is shown in Figure 3C, where the blue pseudo color represents the cell background and the yellow, red, and green colors represent silver-containing vesicles sized in a 200À600 nm range. The highest accumulation was observed at 24 h, as illustrated by the number of colored vesicles or spots ( Figure 4A1À7 and B1À7; Supporting Information Movie, avi file 2 as control, and avi file 3 at 24 h uptake).…”

Section: Articlementioning

confidence: 99%

Use of Synchrotron Radiation-Analytical Techniques To Reveal Chemical Origin of Silver-Nanoparticle Cytotoxicity

et al. 2015

Self Cite

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To predict potential medical value or toxicity of nanoparticles (NPs), it is necessary to understand the chemical transformation during intracellular processes of NPs. However, it is a grand challenge to capture a high-resolution image of metallic NPs in a single cell and the chemical information on intracellular NPs. Here, by integrating synchrotron radiation-beam transmission X-ray microscopy (SR-TXM) and SR-X-ray absorption near edge structure (SR-XANES) spectroscopy, we successfully capture the 3D distribution of silver NPs (AgNPs) inside a single human monocyte (THP-1), associated with the chemical transformation of silver. The results reveal that the cytotoxicity of AgNPs is largely due to the chemical transformation of particulate silver from elemental silver (Ag(0))n, to Ag(+) ions and Ag-O-, then Ag-S- species. These results provide direct evidence in the long-lasting debate on whether the nanoscale or the ionic form dominates the cytotoxicity of silver nanoparticles. Further, the present approach provides an integrated strategy capable of exploring the chemical origins of cytotoxicity in metallic nanoparticles.

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Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

2018

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between individual uptake mechanisms for respective nanoparticle systems. Cells internalizing nanoparticles respond to the nanoparticles and different intracellular processes will be initiated, possibly resulting in the impairment of the cellular key functions. [4] Even though these investigations provide data with statistical relevance, they can merely provide an overall picture of the uptake mechanism, as issues of different uptake mechanisms in anisotropic nanoparticle suspensions are barely assessable. Simultaneously, the influence of the biological environment, e.g., the presence of serum proteins, salts, or altered pH conditions, may have a severe impact on the particle properties, which can entail various biological implications. As such, the role of high resolution imaging techniques, which provide a clear evidence for the nanoparticles' fate in the cellular environment, [5] their localization and targeting to different cellular organelles, as well as evidence for uptake and release mechanisms, have gained increasing importance. In particular, transmission electron microscopy (TEM) investigations provide attractive possibilities to study the interaction of particles with biological barriers, i.e., the extracellular membrane or the nuclear membrane, their accumulation into cellular organelles or their spatial distribution on the scale of individual nanoparticles [6] (e.g., uptake as individual nanoparticles or in form of large clusters, etc.). In addition TEM can simultaneously image also the cellular membranes and organelles to determine respective morphological changes [7] upon nanoparticle interaction. [8][9][10][11] Thus, attractive possibilities to acquire additional information on the particle fate inside the cell emerge from TEM investigations.It can be stated here, that the utilization of electron microscopy has been conducted during the last decades [5] and, in view of some extent electron microscopy investigations of particle uptake, can be regarded in this sense as a routinely applied technique. However, frequently TEM is only utilized to characterize the nanoparticle system or to prove particle internalization. Only a fraction of reported studies utilize TEM analysis to reveal details of nanoparticle uptake and internalization or even deduce uptake pathways from these studies. This might be related to the fact that sometimes the interpretation of TEM data is not straightforward but a number of experimental limitations have to be taken into account for such studies. In a recent review, the advantages and disadvantages of TEM investigations in the context of the The internalization of nanoparticles into mammalian cells relies on a complex interplay of several parameters, which enable and dictate uptake and fate of nanoparticles in the cellular system. Due to the complexity of the involved processes, a careful experimental design has to be developed to elucidate peculiarities of the uptake processes of new nanoparticle systems into cells. Individual parameters can be hardly considered alone but h...

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Multi-platform genotoxicity analysis of silver nanoparticles in the model cell line CHO-K1

Cited by 110 publications

References 59 publications

The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro

The Influence of Silver Nanoparticles on the Blood-Brain and the Blood-Cerebrospinal Fluid Barrier in vitro

Use of Synchrotron Radiation-Analytical Techniques To Reveal Chemical Origin of Silver-Nanoparticle Cytotoxicity

Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

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