Multi-plane Imaging of Neural Activity From the Mammalian Brain Using a Fast-switching Liquid Crystal Spatial Light Modulator

R, Liu; Ball, Neil; Brockill, James; Kuan, Leonard; Millman, Daniel; White, Casey; Leon, Arielle; Williams, Derric; Nishiwaki, Shig; S, de Vries; Larkin, Josh; Sullivan, David T.; Slaughterbeck, Cliff; Farrell, Colin; Saggau, Peter

doi:10.1101/506618

Cited by 6 publications

(5 citation statements)

References 33 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using this strategy, we measured the in vivo calcium activity of ChCs by two-photon imaging. To simultaneously acquire imagery from sparsely distributed cells ( Figure 7B ), we used a multiplane imaging system ( Liu et al, 2018 ) that allowed near-simultaneous monitoring across a range of cortical depth ( Figure 6C ). The imaging procedure followed a standardized awake behaving paradigm ( de Vries et al, 2020 ), during which head-fixed mice were presented with a screen with uniform luminance and allowed to engage in spontaneous behavior.…”

Section: Resultsmentioning

confidence: 99%

“…Calcium activities from those cells were imaged by two-photon excitation using a custom microscope and 940 nm illumination by a Ti:sapphire laser (a Spectra-Physic Insight X3), focused with a 16×/0.8 NA objective (Nikon N16XLWD-PF). This scope has the ability to correct optical aberration (adaptive optics) and quickly switch focal depth by modulating the beam wavefront with a liquid crystal spatial light modulator (SLM, Meadowlark Optics, HSP-512, Liu et al, 2018 ). With this scope, we recorded calcium activities from planes at three different depths (16 or 32 µm apart in depth) in single imaging sessions.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Structure and function of axo-axonic inhibition

Schneider-Mizell

Bodor

Collman

et al. 2021

eLife

View full text Add to dashboard Cite

Inhibitory neurons in mammalian cortex exhibit diverse physiological, morphological, molecular and connectivity signatures. While considerable work has measured the average connectivity of several interneuron classes, there remains a fundamental lack of understanding of the connectivity distribution of distinct inhibitory cell types with synaptic resolution, how it relates to properties of target cells and how it affects function. Here, we used large-scale electron microscopy and functional imaging to address these questions for chandelier cells in layer 2/3 of the mouse visual cortex. With dense reconstructions from electron microscopy, we mapped the complete chandelier input onto 153 pyramidal neurons. We found that synapse number is highly variable across the population and is correlated with several structural features of the target neuron. This variability in the number of axo-axonic ChC synapses is higher than the variability seen in perisomatic inhibition. Biophysical simulations show that the observed pattern of axo-axonic inhibition is particularly effective in controlling excitatory output when excitation and inhibition are co-active. Finally, we measured chandelier cell activity in awake animals using a cell-type specific calcium imaging approach and saw highly correlated activity across chandelier cells. In the same experiments, in vivo chandelier population activity correlated with pupil dilation, a proxy for arousal. Together these results suggest that chandelier cells provide a circuit-wide signal whose strength is adjusted relative to the properties of target neurons.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Structure and function of axo-axonic inhibition

Schneider-Mizell

Bodor

Collman

et al. 2021

eLife

View full text Add to dashboard Cite

show abstract

“…Newly designed objectives lenses offer an extended view into neuronal tissues that can only be leveraged with high-bandwidth recording techniques. SLMs are promising tools providing flexibility to create multiple beams and even allow us to look deeper into tissue using concepts of adaptive optics (Ji et al, 2010;Liu et al, 2018). Similarly, multiplexing techniques combined with sophisticated scanning technologies, large FOV objective lens designs, and excitation beam shaping offer the opportunity to further increase the number of simultaneously recorded neurons to many thousands of neurons (Tsyboulski et al, 2018;Weisenburger et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…An alternative to inertia-limited axial scanners, such as piezo actuators or electrically tunable lenses, are spatial light modulators (SLMs) that are conjugated to the X/Y scanner and the Fourier plane of the objective (Liu et al, 2018). SLMs apply a variety of diffractive illumination patterns to the laser beam and thereby can shift MPM excitation laterally or axially.…”

Section: Scan Lens Pmtsmentioning

confidence: 99%

Wide. Fast. Deep: Recent Advances in Multiphoton Microscopy ofIn VivoNeuronal Activity

2019

View full text Add to dashboard Cite

Multiphoton microscopy (MPM) has emerged as one of the most powerful and widespread technologies to monitor the activity of neuronal networks in awake, behaving animals over long periods of time. MPM development spanned across decades and crucially depended on the concurrent improvement of calcium indicators that report neuronal activity as well as surgical protocols, head fixation approaches, and innovations in optics and microscopy technology. Here we review the last decade of MPM development and highlight how in vivo imaging has matured and diversified, making it now possible to concurrently monitor thousands of neurons across connected brain areas or, alternatively, small local networks with sampling rates in the kilohertz range. This review includes different laser scanning approaches, such as multibeam technologies as well as recent developments to image deeper into neuronal tissues using new, long-wavelength laser sources. As future development will critically depend on our ability to resolve and discriminate individual neuronal spikes, we will also describe a simple framework that allows performing quantitative comparisons between the reviewed MPM instruments. Finally, we provide our own opinion on how the most recent MPM developments can be leveraged at scale to enable the next generation of discoveries in brain function.

show abstract

“…By contrast, rapid remote focus approaches rely on devices that dynamically alter the optical wavefront of the beam prior to the objective, thereby circumventing the need to physically move the objective. Rapid remote focusing has been implemented with electrically tunable lenses (ETLs) [3], liquid crystal spatial light modulators (LC-SLMs) [4][5][6][7][8], phase-locked ultrasound lenses (tunable acoustic gradient (TAG) lenses) [9], deformable mirrors [10], acousto-optic lenses (AOLs) [11][12][13][14] and a secondary objective coupled with a piston mirror [15][16][17][18][19]. However, remote focus devices cannot always be positioned in a plane conjugate to the back aperture of the objective, due to mechanical space constraints, a limited range of commercially available focal length lenses and uncertainty in the exact position of the back aperture of commercial objectives.…”

Section: Introductionmentioning

confidence: 99%

Precompensation of 3D field distortions in remote focus two-photon microscopy

Valera

Neufeldt

Kirkby

et al. 2021

Biomed. Opt. Express

View full text Add to dashboard Cite

Remote focusing is widely used in 3D two-photon microscopy and 3D photostimulation because it enables fast axial scanning without moving the objective lens or specimen. However, due to the design constraints of microscope optics, remote focus units are often located in non-telecentric positions in the optical path, leading to significant depth-dependent 3D field distortions in the imaging volume. To address this limitation, we characterized 3D field distortions arising from non-telecentric remote focusing and present a method for distortion precompensation. We demonstrate its applicability for a 3D two-photon microscope that uses an acousto-optic lens (AOL) for remote focusing and scanning. We show that the distortion precompensation method improves the pointing precision of the AOL microscope to < 0.5 µm throughout the 400 × 400 × 400 µm imaging volume.

show abstract

Multi-plane Imaging of Neural Activity From the Mammalian Brain Using a Fast-switching Liquid Crystal Spatial Light Modulator

Cited by 6 publications

References 33 publications

Structure and function of axo-axonic inhibition

Structure and function of axo-axonic inhibition

Wide. Fast. Deep: Recent Advances in Multiphoton Microscopy ofIn VivoNeuronal Activity

Precompensation of 3D field distortions in remote focus two-photon microscopy

Contact Info

Product

Resources

About