Multi-peptide ELISAs overcome cross-reactivity and inadequate sensitivity of conventional Chlamydia pneumoniae serology

Rahman, K. Shamsur; Kaltenboeck, Bernhard

doi:10.1038/s41598-019-51501-5

Cited by 10 publications

(7 citation statements)

References 52 publications

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“…In ELISA this antigen mixture enables detection in animal sera of antibodies against all other Chlamydia species, as demonstrated in this study with sera from experimentally infected mice and chickens. This is consistent with published observations where such antigens are shown to be seroreactive with antibodies against (all) other Chlamydia species [ 31 ]. The broad nature of the ELISA antigens may also be responsible for another type of cross-reactions: cross-reactions by antibodies against other bacteria that occur in chickens, Chlamydia -related or otherwise.…”

Section: Discussionsupporting

confidence: 93%

Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Wal

Achterberg

Goot

et al. 2023

Vet Res

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The available differentiating tests for Chlamydia are based on detection of genetic material and only give information about the actual infection status, but reveal nothing of past infections. As the use of serological methods increases the window of detection, the goal of this study was to investigate if it is possible to develop a differentiating serological test for antibodies against Chlamydia species in chicken sera. Focus was on C. psittaci, C. gallinacea, and two closely related species, i.e. C. abortus and C. avium. To enable differentiating serology, a bead-based Luminex suspension array was constructed, using peptides as antigens, derived from known immunoreactive Chlamydia proteins. For the majority of these peptides, species-specific seroreactivity in mammalian sera has been reported in literature. The suspension array correctly identified antibodies against various Chlamydia species in sera from experimentally infected mice, and was also able to differentiate between antibodies against C. psittaci and C. gallinacea in sera from experimentally infected chickens. In field sera, signals were difficult to interpret as insufficient sera from experimentally infected chickens were available for evaluating the seroreactivity of all peptides. Nevertheless, results of the suspension array with field sera are supported by published data on the occurrence of C. gallinacea in Dutch layers, thereby demonstrating the proof of concept of multiplex serology for Chlamydial species in poultry.

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Section: Discussionsupporting

confidence: 93%

Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Wal

Achterberg

Goot

et al. 2023

Vet Res

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“…What is the underlying mechanism(s) of action? Cp-specific IgE, 35 , 36 sensitive and specific Cp IgA enzyme-linked immunosorbent assays (ELISAs) 37 , 38 and Mp Community-Acquired Respiratory Distress Syndrome (CARDS) toxin antibodies, 39 available only from research laboratories, are desirable candidates as infection biomarkers and should be considered for inclusion in future randomized clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Outcomes of Antibiotics in Adults with “Difficult to Treat” Asthma or the Overlap Syndrome

Wagshul¹,

Brown²,

Schultek³

et al. 2021

JAA

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Purpose Macrolides are a recommended treatment option for severe asthma, but data for “difficult-to-treat” asthma, the asthma-COPD “overlap” syndrome, and treatment duration beyond one year are lacking. We present long-term data from community practice experience providing insights for practice and research. Methods We report data from (1) baseline (pre-treatment) chart review of antibiotic-treated asthma patients and (2) follow-up telephone interviews documenting severe exacerbations (NIH criteria), Asthma Control Test (ACT) scores, and asthma controller use at baseline and follow-up, analyzed using a “before-after” model. Results A total of 101 patients (mean age 55.6 years (Sd 16.8), 66 females) were included. None had ever taken high dose inhaled corticosteroids and 79 (78.2%) were severely uncontrolled (ACT score ≤15) before treatment. Coexisting COPD was present in 62 (61.4%) patients. Azithromycin or azithromycin plus doxycycline was primarily prescribed with a median treatment duration of 12 months and median follow-up duration of 22 months. Severe exacerbations in the month before treatment occurred in 50.5% vs 17.8% at follow-up (P<0.0001). Mean ACT score increased from 12.2 to 20.6 (P<0.0001). The number of patients taking controller medications decreased (P<0.0001 for inhaled corticosteroids; P<0.001 for long-acting beta agonist/long-acting muscarinic antagonist; P<0.05 for leukotriene receptor antagonists). Of the 79 severely uncontrolled patients, 51 (64.6%) became controlled at follow-up, and of these 51, 27 (52.9%) continued to take antibiotics while 24 (47.1%) had discontinued antibiotics earlier yet remained controlled. Conclusion Antibiotic treatment may be beneficial in a significant proportion of “difficult to treat” asthma patients beyond one year, including some patients with the overlap syndrome and/or who fail to meet criteria for refractoriness.

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“…Interpretation of the results requires expertise and the endpoints are subjective, which restricts the possibility of regulating MIF titers and standardizing it between different laboratories. What is more, this test is retrospective, due to the necessity of obtaining paired serum samples and it is of rather low specificity, thus creating the possibility of invalid diagnosis [53,54]. Its disadvantages may be overcome with ELISA, especially its multi-protein subtype.…”

Section: Chlamydia Pneumoniaementioning

confidence: 99%

Diagnostics of atypical pulmonary infections

Adamczyk¹,

Parkolap²,

Kiryszewska-Jesionek³

et al. 2023

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Atypical community-acquired pneumonia (CAP) is a lung infection caused by atypical bacteria. It is associated with nonspecific symptoms, the course of the disease is unusual and it poses a serious threat to patients. Even though CAP is quite prevalent, most cases remain undiagnosed, and clinicians rely solely on empirical therapy. The aim of this article is to characterize the most frequently used methods in diagnostics of atypical CAPs and evaluate their efficacy. A literature review showed that most of these techniques are still under development and there is a need for standardized diagnostic algorithms for atypical infections. Molecular panels and serological assays have been especially emphasized as they allow for rapid identification of etiologic agents and antibiotic resistance. StreszczenieAtypowe zapalenie płuc (AZP) to infekcja spowodowana przez bakterie atypowe. Charakteryzuje się nietypowym przebiegiem, mało specyficznymi objawami, a jej powikłania mogą nieść duże ryzyko dla pacjentów. Mimo że prewalencja tej choroby jest wysoka, duża część przypadków nie zostaje prawidłowo zdiagnozowana i klinicyści opierają się jedynie na terapii empirycznej. W artykule przedstawiono metody powszechnie stosowane w diagnostyce AZP wraz z krótką charakterystyką i porównanie ich skuteczności. Przegląd literatury wykazał, że większość technik wciąż jest w fazie rozwoju i nie posiadamy wystandaryzowanych algorytmów postępowania w przypadku atypowych infekcji. Szczególny nacisk został położony na panele molekularne i serologiczne, które pozwalają na szybką, skuteczną identyfikację czynnika etiologicznego oraz ocenę lekooporności.

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Multi-peptide ELISAs overcome cross-reactivity and inadequate sensitivity of conventional Chlamydia pneumoniae serology

Cited by 10 publications

References 52 publications

Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Outcomes of Antibiotics in Adults with “Difficult to Treat” Asthma or the Overlap Syndrome

Diagnostics of atypical pulmonary infections

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