Multi-pass, single-molecule nanopore reading of long protein strands
Keisuke Motone,
Daphne Kontogiorgos-Heintz,
Jasmine Wee
et al.
Abstract:The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited in achieving this goal
1
,
2
. Here, we establish a method for the long-range, single-molecule reading of intact protein strands on a commercial nanopore sensor array. By using the ClpX unfoldase to ratchet proteins through a CsgG nanopore
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