Multi‐organ injuries caused by lipopolysaccharide‐induced periodontal inflammation in rats: role of melatonin

Gülle, Kanat; Akpolat, Meryem; Kurçer, Zehra; Cengiz, Murat; Baba, Füsun; Açıkgöz, Şerefden

doi:10.1111/jre.12156

Cited by 21 publications

(28 citation statements)

References 29 publications

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“…Liu et al also demonstrated that melatonin ameliorated the elevated TNF‐α, IL‐1β, IL‐6, iNOS, and COX‐2 levels and decreased the apoptotic cells in hypoxic rats 39 . Gulle et al suggested that melatonin decreased periodontal inflammation caused by lipopolysaccharide in rats with DM 40 . In contrast, in the present study although there was a slight increase, no significant change in IL‐1β or iNOS levels in rats with DM or any positive effect of melatonin on these parameters was observed.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Melatonin on Bone Loss, Diabetic Control, and Apoptosis in Rats With Diabetes With Ligature‐Induced Periodontitis

Yüce

Karataş

Turkal

et al. 2016

Journal of Periodontology

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Section: Discussionmentioning

confidence: 99%

The Effect of Melatonin on Bone Loss, Diabetic Control, and Apoptosis in Rats With Diabetes With Ligature‐Induced Periodontitis

Yüce

Karataş

Turkal

et al. 2016

Journal of Periodontology

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“…Various experimental periodontitis models have been proposed in the extant literature (27)(28)(29)47). Ligatures placed in the dentogingival area increase plaque formation and the amount of bacteria, and induce effective plaque-host interactions (43,48).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have provided evidence of the positive contributions of melatonin and its physiological and pathological implications in the oral cavity (24)(25)(26)(27). Gülle et al (27) reported that melatonin ameliorated periodontal inflammation-induced organ injuries in rats.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis in rats

et al. 2017

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The aim of this study was to evaluate the effects of melatonin on the oxidative stress in heart tissues after induction of experimental periodontitis in rats. Thirty Wistar Albino male rats were divided into four groups as follows: healthy + saline solution (Hs, n = 7), healthy + melatonin (Hm, n = 7), periodontitis + saline solution (Ps, n = 8), and periodontitis + melatonin (Pm, n = 8). Experimental periodontitis was induced using a ligature placed at the gingival margin of the maxillary second molars. Melatonin was applied intraperitoneally (10 mg/ kg) every day for 2 weeks. After sacrificing the rats, serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) levels, and melatonin levels were evaluated. The Pm group exhibited lower alveolar bone loss than the Ps group. Melatonin levels increased in the periodontitis groups, and the Pm group had lower MDA levels and higher GSH-Px levels than the Ps group. These findings suggest that melatonin administration reduces MDA and increases GSH-Px levels in heart tissue, and these effects may be due to its antioxidant properties.Further studies are needed to understand the effects of melatonin on the association between periodontitis and cardiovascular disease.

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“…A cross-sectional study has demonstrated that topical application of melatonin on gingival tissues has a favorable effect on preventing the periodontal disease [12]. In vivo studies were successfully performed with melatonin in periodontal disease models indicating that melatonin could exert protective and preventive effects [13–15]. Choi et al reported that melatonin inhibited Prevotella intermedia LPS-induced inflammation [16].…”

Section: Introductionmentioning

confidence: 99%

Melatonin Receptor Agonists as the “Perioceutics” Agents for Periodontal Disease through Modulation of Porphyromonas gingivalis Virulence and Inflammatory Response

Wei¹,

Zhang²,

Zhu³

et al. 2016

PLoS ONE

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Aim“Perioceutics” including antimicrobial therapy and host modulatory therapy has emerged as a vital adjunctive treatment of periodontal disease. Melatonin level was significantly reduced in patients with periodontal diseases suggesting melatonin could be applied as a potential “perioceutics” treatment of periodontal diseases. This study aims to investigate the effects of melatonin receptor agonists (melatonin and ramelteon) on Porphyromonas gingivalis virulence and Porphyromonas gingivalis-derived lipopolysaccharide (Pg-LPS)-induced inflammation.MethodsEffects of melatonin receptor agonists on Porphyromonas gingivalis planktonic cultures were determined by microplate dilution assays. Formation, reduction, and viability of Porphyromonas gingivalis biofilms were detected by crystal violet staining and MTT assays, respectively. Meanwhile, biofilms formation was also observed by confocal laser scanning microscopy (CLSM). The effects on gingipains and hemolytic activities of Porphyromonas gingivalis were evaluated using chromogenic peptides and sheep erythrocytes. The mRNA expression of virulence and iron/heme utilization was assessed using RT-PCR. In addition, cell viability of melatonin receptor agonists on human gingival fibroblasts (HGFs) was evaluated by MTT assays. After pretreatment of melatonin receptor agonists, HGFs were stimulated with Pg-LPS and then release of cytokines (IL-6 and lL-8) was measured by enzyme-linked immunosorbent assay (ELISA).ResultsMelatonin and ramelteon did exhibit antimicrobial effects against planktonic culture. Importantly, they inhibited biofilm formation, reduced the established biofilms, and decreased biofilm viability of Porphyromonas gingivalis. Furthermore, they at sub-minimum inhibitory concentration (sub-MIC) concentrations markedly inhibited the proteinase activities of gingipains and hemolysis in a dose-dependent manner. They at sub-MIC concentrations significantly inhibited the mRNA expression of virulence factors (kgp, rgpA, rgpB, hagA, and ragA), while increasing the mRNA expression of ferritin (ftn) or hemolysin (hem). They did not show obvious cytotoxicity toward HGFs. They inhibited Pg-LPS-induced IL-6 and IL-8 secretion, which was reversed by luzindole, the melatonin receptor antagonist.ConclusionMelatonin receptor agonists can inhibit planktonic and biofilm growth of Porphyromonas gingivalis by affecting the virulent properties, as well as Pg-LPS-induced inflammatory response. Our study provides new evidence that melatonin receptor agonists might be useful as novel “perioceutics” agents to prevent and treat Porphyromonas gingivalis-associated periodontal diseases.

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Multi‐organ injuries caused by lipopolysaccharide‐induced periodontal inflammation in rats: role of melatonin

Abstract: The results revealed that increased serum AST, ALT and BUN levels following periodontitis are ameliorated with melatonin treatment.

Cited by 21 publications

References 29 publications

The Effect of Melatonin on Bone Loss, Diabetic Control, and Apoptosis in Rats With Diabetes With Ligature‐Induced Periodontitis

The Effect of Melatonin on Bone Loss, Diabetic Control, and Apoptosis in Rats With Diabetes With Ligature‐Induced Periodontitis

Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis in rats

Melatonin Receptor Agonists as the “Perioceutics” Agents for Periodontal Disease through Modulation of Porphyromonas gingivalis Virulence and Inflammatory Response

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