Multi-omics reveals aspirin eugenol ester alleviates neurological disease

Tao, Qi; Zhang, Zhendong; Lu, Xiao-Rong; Qin, Zhe; Liu, Xi-Wang; Li, Shihong; Bai, Lin; Ge, Bing-Chen; Li, Jianyong; Yang, Yajun

doi:10.1016/j.biopha.2023.115311

Cited by 1 publication

(3 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method for measuring the axonal growth of SH-SY5Y cells was carried out according to our previous experimental steps [ 15 ]. In brief, 2 × 10 4 SH-SY5Y cells were seeded in 6-well plates.…”

Section: Methodsmentioning

confidence: 99%

“…The expressions of β-actin, neurofilament medium polypeptide (NEFM), and brain-derived neurotrophic factor (BDNF) were analyzed in SH-SY5Y cells via Western blot. The method for determining protein expression follows our previous experimental steps [ 15 ]. In brief, the collected total protein was separated using a precast SDS-PAGE gel.…”

Section: Methodsmentioning

confidence: 99%

“…The TEM determination of exosomes separated with ultracentrifugation refers to our previous experimental steps [ 15 ]. In brief, one drop of resuspended exosomes was put on a copper mesh for 5 min.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

MicroRNA in the Exosomes Mediated by Resveratrol to Activate Neuronal Cells

Zhang,

Tao,

Bai

et al. 2024

Toxics

Self Cite

View full text Add to dashboard Cite

Resveratrol (RSV), a polyphenol, is known to have a wide range of pharmacological properties in vitro. RSV may have therapeutic value for various neurodegenerative diseases via neuroprotective effects. However, it is not yet clear whether RSV can induce intestinal–brain interactions. It is assumed that the intestinal cells may secrete some factors after being stimulated by other substances. These secreted factors may activate nerve cells through gut–brain interaction, such as exosomes. In this study, it was discovered that Caco-2 cells treated with RSV secrete exosomes to activate SH-SY5Y neuronal cells. The results showed that secreted factors from RSV-treated Caco-2 cells activated SH-SY5Y. The exosomes of RSV-treated Caco-2 cells activated SH-SY5Y cells, which was manifested in the lengthening of the nerve filaments of SH-SY5Y cells. The exosomes were characterized using transmission electron microscopy and sequenced using the Illumina NovaSeq 6000 sequencer. The results showed that the miRNA expression profile of exosomes after RSV treatment changed, and twenty-six kinds of miRNAs were identified which expressed differentially between the control group and the RSV-treated group. Among them, three miRNAs were selected as candidate genes for inducing SH-SY5Y neural cell activation. Three miRNA mimics could activate SH-SY5Y neurons. These results suggested that the miRNA in intestinal exocrine cells treated with RSV may play an important role in the activation of SH-SY5Y neurons.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%