Multi-Omics Integration Reveals the Crucial Role of
            <i>Fusobacterium</i>
            in the Inflammatory Immune Microenvironment in Head and Neck Squamous Cell Carcinoma

Han, Qiao; Li, Hui; Wen, Xiang; Tan, Xiao-Ying; Yang, Chengzhang; Liu, Na

doi:10.1128/spectrum.01068-22

Cited by 12 publications

(12 citation statements)

References 48 publications

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“…Furthermore, the disturbance of the oral microbiome may indirectly promote the occurrence and development of cancers by causing persistent chronic inflammation and suppressing immune response [ 50 , 51 ]. Several studies have shown that the periodontal pathogen Fusobacterium nucleatum might increase the risk of head and neck cancers by indirect carcinogenic effects [ 52 , 53 ]. A recent study revealed that CD8 + T infiltration was decreased in NPC tumor tissues with high bacterial load, indicating that the microbiome may influence NPC by modulating the immune microenvironment [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

The Oral Microbiome as Mediator between Oral Hygiene and Its Impact on Nasopharyngeal Carcinoma

Liu

Liao

et al. 2023

Microorganisms

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Oral hygiene and the alteration of the oral microbiome have been linked to nasopharyngeal carcinoma (NPC). This study aimed to investigate whether the oral microbiome plays a mediating role in the relationship between oral hygiene and NPC, and identify differential microbial taxonomies that potentially mediated this association. We conducted a case–control study that involved 218 NPC patients and 192 healthy controls. The 16S rRNA gene sequencing of the V4 region was performed to evaluate the composition of the oral microbiome. Mediation analysis was applied to explore the relationship among oral hygiene, the oral microbiome and NPC. We found that dental fillings and poor oral hygiene score were associated with increased risks of NPC (OR = 2.51 (1.52–4.25) and OR = 1.54 (1.02–2.33)). Mediation analysis indicated that dental fillings increased the risk of NPC by altering the abundance of Erysipelotrichales, Erysipelotrichaceae, Solobacterium and Leptotrichia wadei. In addition, Leptotrichia wadei also mediated the association between oral hygiene score and the risk of NPC. Our study confirmed that poor oral hygiene increased the risk of NPC, which was partly mediated by the oral microbiome. These findings might help us to understand the potential mechanism of oral hygiene influencing the risk of NPC via the microbiome.

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Section: Discussionmentioning

confidence: 99%

The Oral Microbiome as Mediator between Oral Hygiene and Its Impact on Nasopharyngeal Carcinoma

Liu

Liao

et al. 2023

Microorganisms

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“…SH2 domain containing 5, and solute carrier family 31 member 2. 25 These genes exert chronic inflammatory effects through the increased activation of NF-κB and inflammation, cell-cell adhesion, and other pathways, resulting in an immunosuppressive TME. 25…”

Section: Itm Reshapes the Tme For Tumor Developmentmentioning

confidence: 99%

“…In head and neck squamous cell carcinoma, the enrichment of Clostridium spp. is positively associated with inflammatory genes, including epiregulin, proline rich and Gla domain 1, SH2 domain containing 5, and solute carrier family 31 member 2 25 . These genes exert chronic inflammatory effects through the increased activation of NF‐κB and inflammation, cell‐cell adhesion, and other pathways, resulting in an immunosuppressive TME 25 .…”

Section: Interplay Between Itm and The Tme In Tumor Developmentmentioning

confidence: 99%

“…is positively associated with inflammatory genes, including epiregulin, proline rich and Gla domain 1, SH2 domain containing 5, and solute carrier family 31 member 2 25 . These genes exert chronic inflammatory effects through the increased activation of NF‐κB and inflammation, cell‐cell adhesion, and other pathways, resulting in an immunosuppressive TME 25 . ITM further promote cancer cell metastasis and invasive cell dormancy by activating the transcription factor Jun proto‐oncogene and Fos proto‐oncogene families, recruiting myeloid cells, inducing inflammatory responses through Janus tyrosine kinase‐signal transducer and activator of transcription (JAK‐STAT) signaling, and reducing T‐cell activity by releasing ILs and chemokines into the TME 26 .…”

Section: Interplay Between Itm and The Tme In Tumor Developmentmentioning

confidence: 99%

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The value of intratumoral microbiota in the diagnosis and prognosis of tumors

Zhou,

Zhang,

Fan

et al. 2024

Cell Biochemistry & Function

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Intratumoral microbiota (ITM) are microorganisms present in tumor cells. ITM participate in tumor development by affecting tumor cells directly and the tumor microenvironment (TME), indirectly. Alterations in ITM instigate changes in tumor DNA, activate oncogenic pathways, induce tumor inflammatory responses, disrupt normal immune activity, and facilitate the secretion of effectors leading to tumor progression, metastasis, or diminished therapeutic effects. ITM varies significantly in different types of cancer cells and disease states. The presence of certain ITM serves as a predictor of various disease states. Thus, ITM predicts tumorigenesis, tumor grade, treatment efficacy, and prognosis, making it a potential tumor biomarker. The present study aimed to determine the mechanisms by which ITM affects tumor development, especially through the TME; highlight the significant potential of ITM in enhancing tumor diagnosis and prognosis; and outline future directions for ITM research, with a focus on the development of innovative tumor markers.

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“…There are multiple reports describing the microbiota in HNSC [26][27][28]. Most of these studies compared the microbiota diversity and bacterial relative abundance between cancer and healthy samples using 16S ribosomal RNA (rRNA) sequencing [26][27][28], while two studies additionally correlated the impact of the microbiota with matched transcriptome analysis [29,30]. Samples studied include tissues, swabs and oral fluids (saliva or oral rinse) from cancer and healthy patients.…”

Section: Introductionmentioning

confidence: 99%

Identification of consensus head and neck cancer-associated microbiota signatures: a systematic review and meta-analysis of 16S rRNA and The Cancer Microbiome Atlas datasets

Yeo,

Li,

et al. 2024

Journal of Medical Microbiology

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Introduction. Multiple reports have attempted to describe the tumour microbiota in head and neck cancer (HNSC). Gap statement. However, these have failed to produce a consistent microbiota signature, which may undermine understanding the importance of bacterial-mediated effects in HNSC. Aim. The aim of this study is to consolidate these datasets and identify a consensus microbiota signature in HNSC. Methodology. We analysed 12 published HNSC 16S rRNA microbial datasets collected from cancer, cancer-adjacent and non-cancer tissues to generate a consensus microbiota signature. These signatures were then validated using The Cancer Microbiome Atlas (TCMA) database and correlated with the tumour microenvironment phenotypes and patient’s clinical outcome. Results. We identified a consensus microbial signature at the genus level to differentiate between HNSC sample types, with cancer and cancer-adjacent tissues sharing more similarity than non-cancer tissues. Univariate analysis on 16S rRNA datasets identified significant differences in the abundance of 34 bacterial genera among the tissue types. Paired cancer and cancer-adjacent tissue analyses in 16S rRNA and TCMA datasets identified increased abundance in Fusobacterium in cancer tissues and decreased abundance of Atopobium , Rothia and Actinomyces in cancer-adjacent tissues. Furthermore, these bacteria were associated with different tumour microenvironment phenotypes. Notably, high Fusobacterium signature was associated with high neutrophil (r=0.37, P<0.0001), angiogenesis (r=0.38, P<0.0001) and granulocyte signatures (r=0.38, P<0.0001) and better overall patient survival [continuous: HR 0.8482, 95 % confidence interval (CI) 0.7758–0.9273, P=0.0003]. Conclusion. Our meta-analysis demonstrates a consensus microbiota signature for HNSC, highlighting its potential importance in this disease.

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Multi-Omics Integration Reveals the Crucial Role of Fusobacterium in the Inflammatory Immune Microenvironment in Head and Neck Squamous Cell Carcinoma

Cited by 12 publications

References 48 publications

The Oral Microbiome as Mediator between Oral Hygiene and Its Impact on Nasopharyngeal Carcinoma

The Oral Microbiome as Mediator between Oral Hygiene and Its Impact on Nasopharyngeal Carcinoma

The value of intratumoral microbiota in the diagnosis and prognosis of tumors

Identification of consensus head and neck cancer-associated microbiota signatures: a systematic review and meta-analysis of 16S rRNA and The Cancer Microbiome Atlas datasets

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