Multi-omics-based identification of purple acid phosphatases and metabolites involved in phosphorus recycling in stylo root exudates

Wu, Yuanhang; Zhao, Cang; Zhao, Xingkun; Yang, Liyun; Li, Chun; Jiang, Lingyan; Liu, Guodao; Liu, Pandao; Luo, Lijuan

doi:10.1016/j.ijbiomac.2023.124569

Cited by 10 publications

(7 citation statements)

References 51 publications

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“…Zhou et al (2016) reported that the acid phosphatase activities in the root extract of five low‐P‐tolerant soybean cultivars increased under low‐P conditions. Wu et al (2023) also reported that the secretion of phosphatases increased under low‐P conditions. In the present study, low‐P‐tolerant cultivars released high levels of acid phosphatase from 7 to 14 DAT as an adaptation to low‐P levels.…”

Section: Discussionmentioning

confidence: 90%

“…The low-P tolerance of plants consists of plant's ability (1) to acquire P from soil with low-P availability and (2) to utilize absorbed P in their cells and organs. The P acquisition mechanisms in plants include: alteration in the root system architecture (Lopez et al, 2023), secretion of acid phosphatase from roots (Wu et al, 2023), and secretion of organic acids from roots (Li et al, 2022). Moreover, metabolomics has been widely used to reveal the response of different crops under low phosphorus stress, such as soybean (Mo et al, 2019), maize (Luo et al, 2019), pigeon pea (Liu et al, 2022), and rice (Yan et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Tantriani,

Cheng,

Oikawa

et al. 2023

Physiologia Plantarum

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Phosphorus (P) deficiency alters the root morphological and physiological traits of plants. This study investigates how soybean cultivars with varying low‐P tolerance values respond to different P levels in hydroponic culture by assessing alterations in root length, acid phosphatase activity, organic acid exudation, and metabolites in root exudates. Three low‐P‐tolerant cultivars (‘Maetsue,’ ‘Kurotome,’ and ‘Fukuyutaka’) and three low‐P‐sensitive cultivars (‘Ihhon,’ ‘Chizuka,’ and ‘Komuta’) were grown under 0 (P0) and 258 μM P (P8) for 7 and 14 days after transplantation (DAT). Low‐P‐tolerant cultivars increased root length by 31% and 119%, which was lower than the 62% and 144% increases in sensitive cultivars under P0 compared to P8 at 7 and 14 DAT, respectively. Acid phosphatase activity in low‐P‐tolerant cultivars exceeded that in sensitive cultivars by 5.2‐fold and 2.0‐fold at 7 and 14 DAT. Root exudates from each cultivar revealed 177 metabolites, with higher organic acid exudation in low‐P‐tolerant than sensitive cultivars under P0. Low‐P‐tolerant cultivars increased concentrations of specific metabolites (oxalate, GABA, quinate, citrate, AMP, 4‐pyridoxate, and CMP), distinguishing them from low‐P‐sensitive cultivars under P0. The top five metabolomic pathways (purine metabolism, arginine and proline metabolism, TCA cycle, glyoxylate and dicarboxylate metabolism, alanine, aspartate, and glutamate metabolism) were more pronounced in low‐P‐tolerant cultivars at 14 DAT. These findings indicate that increasing root length was not an adaptation strategy under P deficiency; instead, tolerant cultivars exhibit enhanced root physiological traits, including increased acid phosphatase activity, organic acid exudation, specific metabolite release, and accelerated metabolic pathways under P deficiency.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Tantriani,

Cheng,

Oikawa

et al. 2023

Physiologia Plantarum

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“…In the process of mobilizing phosphorus from organic soil compounds, the main role is attributed to enzymes localized in the cell walls of several plant species and secreted into the soil, called purple acid phosphatases (a subgroup of acid phosphatases, EC 3.1.3.2). Their ability to release phosphate ions from various monoesters, such as mononucleotides, lower inositol phosphates, sugar phosphates, etc., is known, and under conditions of phosphorus starvation, their exudation from roots increases [63,64]. However, in other plant species, which do not suffer from phosphorus limitation, the function of these enzymes in phosphorus mobilization is recognized to be minimal [65].…”

Section: Release Of Phosphate Ions From Soil Components: Potential Ca...mentioning

confidence: 99%

Phosphorus Supply to Plants of Vaccinium L. Genus: Proven Patterns and Unexplored Issues

Struchkova,

Mikheev,

Berezina

et al. 2024

Agronomy

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Phosphorus availability is a serious problem for plants growing and grown in acidic soils of bogs, poor in macronutrients. The application of phosphorus fertilizers to such soils is unprofitable because of the physical and chemical properties of these soils, where phosphate is firmly bound to organic and inorganic compounds and becomes inaccessible to plants. Plants of the Vaccinium genus both from natural stands and commercial plantations may suffer from phosphorus deficiency, so they need to have a number of adaptations that allow them to efficiently extract phosphorus. This review addresses the following issues in relation to plants of the Vaccinium genus: sources of phosphorus for plants; the release of phosphate ions from soil components; the transport of phosphate ions into plants; and the importance of mycorrhiza in supplying phosphorus to plants. Thus, we sought to draw researchers’ attention to sources and routes of phosphorus supply of plants of the Vaccinium genus and its unexplored aspects.

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“…The findings of previous studies have unequivocally demonstrated that a low-phosphorus environment contributes to the growth of roots, increases the APase activity secreted by roots, and promotes the efflux of flavonoids and organic acids exerted by roots, thereby promoting the recycling of insoluble phosphorus and organic phosphorus in soil [13]. Another study showed that maize lines from Embrapa can significantly alter root morphology under low-phosphorus conditions to enhance plant adaptability to low-phosphorus soils, while maize lines from DTMA reduce root length and surface area under phosphorus-deficient conditions [14], but the mechanism for this difference in peanuts has been scarcely studied.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Physiological Mechanism of Activating Insoluble Inorganic Phosphorus of Different Peanut (Arachis hypogaea L.) Varieties under Low Phosphorus

Tan,

Liu,

Wan

et al. 2023

Agriculture

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To reduce the application of phosphorus fertilizer and improve phosphorus efficiency in peanut (Arachis hypogaea L.) production, six peanut varieties with different phosphorus activation efficiencies were selected, and the root morphology, physiological indexes, and types and content of organic acids secreted were measured via a hydroponic experiment for 20 days. We analyzed the difference in calcium phosphate activation between peanut seedlings cultivated under low-phosphorus (LP, 0.01 mmol/L KH2PO4) and normal phosphorus (NP, 0.6 mmol/L KH2PO4) conditions and explored the physiological mechanisms of different peanut varieties on the activation efficiency of insoluble inorganic phosphorus. The results showed that under LP conditions, the root length, root surface area, root volume, root tip number, and root activity of the efficient P activation varieties were 18.31%, 17.50%, 15.23%, 20.00%, and 50.90% higher than those of the inefficient P activation varieties respectively. The reduction range of the nutrient solution pH of the high-efficiency varieties was 74.48% higher than that of the low-efficiency varieties under LP conditions. The total amount of organic acid secreted by the efficient P activation varieties increased by 236.07% on average under LP conditions compared with that under NP conditions. In comparison, the average increase in inefficient P activation varieties was only 16.36%. Under low P stress, the peanut varieties with high-efficiency P activation could increase the activation of insoluble inorganic P in the environment mainly by changing the root architecture and increasing the root-shoot ratio, root activity, and root proton and organic acid secretion.

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Multi-omics-based identification of purple acid phosphatases and metabolites involved in phosphorus recycling in stylo root exudates

Cited by 10 publications

References 51 publications

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Phosphorus Supply to Plants of Vaccinium L. Genus: Proven Patterns and Unexplored Issues

Morphological and Physiological Mechanism of Activating Insoluble Inorganic Phosphorus of Different Peanut (Arachis hypogaea L.) Varieties under Low Phosphorus

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