Multi-omics approaches revealed the therapeutic mechanisms of Suo-Quan-Wan for treating overactive bladder in spontaneously hypertensive rats

Sun, Zhihui; Tie, Yan; Tong, Xinyi; Cheng, Mingchang; Wu, Yushan; Xu, Pingxiang; Xue, Ming; Xu, Liping; Zhou, Xuelin

doi:10.1016/j.jep.2023.117066

Cited by 1 publication

(1 citation statement)

References 25 publications

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“…Harvested bladder tissues were homogenized in the lysis buffer (20 mM HEPES-KOH, pH 7.5, 10 mM KCl, 1.5 mM MgCl 2 , 0.2 mM EDTA, 0.1% Triton-X100), with protein concentrations calibrated with a BCA kit (Pierce) 48 . 20 μg proteins were loaded onto sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and blotted onto polyvinylidene fluoride (PVDF) membranes 49 .…”

Section: Western Blot Analysesmentioning

confidence: 99%

Nanocluster-antibody-drug conjugates (NADC) as an intravesical precision theranostic agent for interstitial cystitis

Lin,

Wang,

Liu

et al. 2024

Preprint

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Interstitial cystitis (IC) is a chronic inflammatory disorder characterized by recurring severe pain in the bladder and surrounding pelvic areas, lacking timely diagnostic and therapeutic options. Here, we propose a unitary theranostic nanocluster-antibody-drug conjugate (NADC) by covalently placing dihydroorotate dehydrogenase inhibitors (DHODHi) and ultrasmall gold quantum clusters (AuQCs) on a nerve growth factor (NGF) antagonistic antibody with simultaneous X-ray computed tomographic and near-infrared fluorescence imaging contrasts. Combining anti-inflammatory effects from all individual components, intravesical NADC specifically homed to bladder mucosal lesions and capably alleviated inflammation in chronic, acute, and prophylactic IC models of rats, as revealed by behavioral and pathological evaluations. Transcriptomics unveiled cytokine modulation and concomitant inhibition of perturbed IL-17, NF-κB, TNF, and JAK-STAT signaling pathways. Interestingly, the NADC reconstructed the host bladder microbiota by differentially varying anti-inflammatory and pro-inflammatory bacteria diversities. Distinct from conventional nanoparticles conjugated with antibodies and drugs, NADC relies on the antibody framework and represents a state-of-the-art category of precision theranostic agents with translational potential for diagnosing and treating IC patients.

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Section: Western Blot Analysesmentioning

confidence: 99%