Multi-omics approach reveals dysregulated genes during hESCs neuronal differentiation exposure to paracetamol

Citalopram exposure of hESCs during neuronal differentiation identifies dysregulated genes involved in neurodevelopment and depression

“…The citalopram-induced increase in FRZB expression was identified in both bulk RNA-seq time- and dose-response analysis and in scRNA-seq pseudotime analysis. Interestingly, FRZB , was downregulated in paracetamol-exposed cells using the same neuronal differentiation model, albeit at different time points ( Spildrejorde et al, 2023 ), indicating that the WNT-pathway respond differently to citalopram compared to paracetamol. Another gene that was differentially expressed in both paracetamol-exposed cells and citalopram-exposed cells was neuronal transcription factor PAX6 .…”

“…Another gene that was differentially expressed in both paracetamol-exposed cells and citalopram-exposed cells was neuronal transcription factor PAX6 . In paracetamol-exposed cells PAX6 was upregulated, indicating a delay in neuronal differentiation ( Spildrejorde et al, 2023 ). In contrast, PAX6 expression was downregulated in citalopram-exposed cells in a dose-dependent manner, suggesting that citalopram may enhance neuronal differentiation.…”

“…To study the in vitro effects of citalopram, HS360 hESCs cells were differentiated according to Samara and Spildrejorde et al, 2023 ; Samara et al, 2022a ) with minor changes. Briefly, 70,000 cells/well were seeded in Geltrex pre-coated 12-well plates at Day 0.…”