Multi-Omics Analysis Reveals the Resistance Mechanism and the Pathogens Causing Root Rot of
            <i>Coptis chinensis</i>

Song, Xuhong; Mei, Pengying; Dou, Tao; Liu, Qundong; Li, Longyun

doi:10.1128/spectrum.04803-22

Cited by 5 publications

(2 citation statements)

References 51 publications

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“…These markers encompass a wide range of regions within the potato chromosome, many of which are correlated with disease resistance metabolites and associated pathways, including acylsugar acyltransferase, alkaloid biosynthesis, phenolic biosynthesis, and peroxidases. Our findings are in line with recent findings where acylsugar metabolism, alkaloid biosynthesis, and phenolic biosynthesis is shown to be associated with plant-microbe interaction and disease resistance (Mandal et al, 2020;Song et al, 2023;Yuan et al, 2023). In summary, the integration of two robust approaches, genetic analysis, and metabolomics, has shed light on the biochemical pathways associated with soft rot and blackleg diseases resistance.…”

Section: Discussionsupporting

confidence: 93%

Plant necrotrophic bacterial disease resistance phenotypes, QTL, and metabolites identified through integrated genetic mapping and metabolomics in Solanum species

Joshi,

Paudel,

Eddy

et al. 2024

Front. Plant Sci.

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Most food crops are susceptible to necrotrophic bacteria that cause rotting and wilting diseases in fleshy organs and foods. All varieties of cultivated potato (Solanum tuberosum L.) are susceptible to diseases caused by Pectobacterium species, but resistance has been demonstrated in wild potato relatives including S. chacoense. Previous studies demonstrated that resistance is in part mediated by antivirulence activity of phytochemicals in stems and tubers. Little is known about the genetic basis of antivirulence traits, and the potential for inheritance and introgression into cultivated potato is unclear. Here, the metabolites and genetic loci associated with antivirulence traits in S. chacoense were elucidated by screening a sequenced S. tuberosum x S. chacoense recombinant inbred line (RIL) population for antivirulence traits of its metabolite extracts. Metabolite extracts from the RILs exhibited a quantitative distribution for two antivirulence traits that were positively correlated: quorum sensing inhibition and exo-protease inhibition, with some evidence of transgressive segregation, supporting the role of multiple loci and metabolites regulating these resistance-associated systems. Metabolomics was performed on the highly resistant and susceptible RILs that revealed 30 metabolites associated with resistance, including several alkaloids and terpenes. Specifically, several prenylated metabolites were more abundant in resistant RILs. We constructed a high-density linkage map with 795 SNPs mapped to 12 linkage groups, spanning a length of 1,507 cM and a density of 1 marker per 1.89 cM. Genetic mapping of the antivirulence and metabolite data identified five quantitative trait loci (QTLs) related to quorum sensing inhibition that explained 8-28% of the phenotypic variation and two QTLs for protease activity inhibition that explained 14-19% of the phenotypic variation. Several candidate genes including alkaloid, and secondary metabolite biosynthesis that are related to disease resistance were identified within these QTLs. Taken together, these data support that quorum sensing inhibition and exo-protease inhibition assays may serve as breeding targets to improve resistance to nectrotrophic bacterial pathogens in potato and other plants. The identified candidate genes and metabolites can be utilized in marker assisted selection and genomic selection to improve soft- rot and blackleg disease resistance.

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Section: Discussionsupporting

confidence: 93%

Plant necrotrophic bacterial disease resistance phenotypes, QTL, and metabolites identified through integrated genetic mapping and metabolomics in Solanum species

Joshi,

Paudel,

Eddy

et al. 2024

Front. Plant Sci.

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“…Plants are still an important source of bioactive alkaloids, therefore it is necessary to understand the regulatory mechanisms of alkaloids. In previous studies, the molecular mechanisms of the seven major isoquinoline alkaloids have been explored based on the differences in accumulation among different tissues [15], differences in plants among different regions [16], and the effects of biotic stresses on the growth and development of C. chinensis [17]. However, growth age is a very critical factor affecting the growth and development of cultivated C. chinensis.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolome Analysis of Isoquinoline Alkaloid Biosynthesis of Coptis chinensis in Different Years

Min,

Zhu,

et al. 2023

Genes

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Coptis chinensis is a perennial herb of the Ranunculaceae family. The isoquinoline alkaloid is the main active component of C. chinensis, mainly exists in its rhizomes and has high clinical application potential. The in vitro synthesis of isoquinoline alkaloids is difficult because their structures are complex; hence, plants are still the main source of them. In this study, two-year and four-year rhizomes of C. chinensis were selected to investigate the effect of growth years on the accumulation of isoquinoline alkaloids. Two-year and four-year C. chinensis were selected for metabolomics detection and transcriptomic analysis. A total of 413 alkaloids were detected by metabolomics analysis, of which 92 were isoquinoline alkaloids. (S)-reticuline was a significantly different accumulated metabolite of the isoquinoline alkaloids biosynthetic pathway in C. chinensis between the two groups. The results of transcriptome analysis showed that a total of 464 differential genes were identified, 36 of which were associated with the isoquinoline alkaloid biosynthesis pathway of C. chinensis. Among them, 18 genes were correlated with the content of important isoquinoline alkaloids. Overall, this study provided a comprehensive metabolomic and transcriptomic analysis of the rapid growth stage of C. chinensis rhizome from the perspective of growth years. It brought new insights into the biosynthetic pathway of isoquinoline alkaloids and provided information for utilizing biotechnology to improve their contents in C. chinensis.

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Control of coptis root rot by combination of Bacillus cereus isolate Y9 and other antagonistic microorganisms

Mei,

Dou,

Song

et al. 2024

J Plant Pathol

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Root rot is a destructive soil-borne disease of Coptis chinensis, which depends on chemical control at present, and more attention should be paid to biocontrol of disease. In the present research, isolate Y9 isolated from healthy root samples of Coptis, was identified as Bacillus cereus. Further screening and pot experiments showed that B. cereus isolate Y9 inhibited the growth of the main causal agents of coptis root rot disease (Fusarium solani and F. avenaceum) and seven other phytopathogenic fungi. The application of B. cereus isolate Y9 and compatible Trichoderma harzianum, T. atroviride and B. amyloliquefaciens, singly and in combination were found to be effective against Fusarium root rot in vitro and in field experiments. In field experiments, combinations of T. harzianum + B. amyloliquefaciens + Y9 (HYJ, in ratio of 1:1:1) showed the highest control efficacy of 63.85%, which was higher than the expected value (53.18%), indicating synergistic effect on the control of coptis root rot. Therefore, B. cereus isolate Y9 may be a potential biological control agent, and combined use with T. harzianum and B. amyloquefaciens offered even greater potential. The long-term effects of isolate B. cereus Y9 and its combinations on C. chinensis should be assessed in different locations and seasons in the future.

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Multi-Omics Analysis Reveals the Resistance Mechanism and the Pathogens Causing Root Rot of Coptis chinensis

Cited by 5 publications

References 51 publications

Plant necrotrophic bacterial disease resistance phenotypes, QTL, and metabolites identified through integrated genetic mapping and metabolomics in Solanum species

Plant necrotrophic bacterial disease resistance phenotypes, QTL, and metabolites identified through integrated genetic mapping and metabolomics in Solanum species

Transcriptome and Metabolome Analysis of Isoquinoline Alkaloid Biosynthesis of Coptis chinensis in Different Years

Control of coptis root rot by combination of Bacillus cereus isolate Y9 and other antagonistic microorganisms

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