Multi-omics Analysis of Young Portulaca oleracea L. Plants’ Responses to High NaCl Doses Reveals Insights into Pathways and Genes Responsive to Salinity Stress in this Halophyte Species

Silva, Vivianny Nayse Belo; Silva, Thalliton Luiz Carvalho da; Ferreira, Thalita Massaro Malheiros; Neto, Jorge Candido Rodrigues; Leão, André Pereira; Ribeiro, José Antônio de Aquino; Abdelnur, Patrícia Verardi; Valadares, Leonardo Fonseca; Sousa, Carlos Antônio Ferreira de; Júnior, Manoel Teixeira Souza

doi:10.1007/s43657-022-00061-2

Cited by 5 publications

(19 citation statements)

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“…The B1 accession of purslane ( Portulaca oleracea L. ) used in this study belongs to the Purslane Collection at Embrapa Agroenergia. Seeds underwent disinfection following the same procedure described in Silva et al. (2022) , which consisted of soaking in a solution of 2% sodium hypochlorite and Tween® 20 for 5 min, under slow agitation, and subsequent washing with sterile water and drying on sterilized filter paper.…”

Section: Methodsmentioning

confidence: 99%

“…During the entire experiment, plants were at field capacity. To avoid the loss of Na + or Cl − , no leakage of the saline solution was allowed to get out of the plastic cup, as described previously in Silva et al. (2022) .…”

Section: Methodsmentioning

confidence: 99%

“…A robust multi-omics database on the response of purslane to salt stress and its subsequent use via an MOI analysis can create the basis to decode the genetics behind its resistance to salinity stress via a system biology approach ( Silva et al., 2022 ). Most recently, a few reports on single-omics analysis (SOA) of purslane have been published, including Xing et al.…”

Section: Introductionmentioning

confidence: 99%

“…The present study is a second step in building a robust database on the morpho-physiological and molecular responses of Portulaca oleracea L. to salinity stress and its subsequent use in attempting to decode the genetics behind its resistance to this abiotic stress. After reporting on the characterization of the morpho-physiological responses of young purslane plants to such stress using a robust salinization protocol ( Silva et al., 2022 ), here, we report a study on adult plants through the characterization of the proteome and untargeted metabolome profiles on the leaves and roots of this halophyte species submitted to very high salinity stress and the consequent use of single- and multi-omics analysis strategies to study it.…”

Section: Introductionmentioning

confidence: 99%

“…Second, it sought to characterize the metabolome and proteome in the leaves and roots of adult purslane plants. Due to root size in young plants, that was not possible before ( Silva et al., 2022 ). Last, it attempted to integrate metabolome and proteome profiles, aiming to advance in identifying those pathways most affected by this stress in the leaves and the roots.…”

Section: Introductionmentioning

confidence: 99%

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Osmoprotectants play a major role in the Portulaca oleracea resistance to high levels of salinity stress—insights from a metabolomics and proteomics integrated approach

et al. 2023

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IntroductionPurslane (Portulaca oleracea L.) is a non-conventional food plant used extensively in folk medicine and classified as a multipurpose plant species, serving as a source of features of direct importance to the agricultural and agri-industrial sectors. This species is considered a suitable model to study the mechanisms behind resistance to several abiotic stresses including salinity. The recently achieved technological developments in high-throughput biology opened a new window of opportunity to gain additional insights on purslane resistance to salinity stress—a complex, multigenic, and still not well-understood trait. Only a few reports on single-omics analysis (SOA) of purslane are available, and only one multi-omics integration (MOI) analysis exists so far integrating distinct omics platforms (transcriptomics and metabolomics) to characterize the response of purslane plants to salinity stress.MethodsThe present study is a second step in building a robust database on the morpho-physiological and molecular responses purslane to salinity stress and its subsequent use in attempting to decode the genetics behind its resistance to this abiotic stress. Here, the characterization of the morpho-physiological responses of adult purslane plants to salinity stress and a metabolomics and proteomics integrative approach to study the changes at the molecular level in their leaves and roots is presented.Results and discussionAdult plants of the B1 purslane accession lost approximately 50% of the fresh and dry weight (from shoots and roots) whensubmitted to very high salinity stress (2.0 g of NaCl/100 g of the substrate). The resistance to very high levels of salinity stress increases as the purslane plant matures, and most of the absorbed sodium remains in the roots, with only a part (~12%) reaching the shoots. Crystal-like structures, constituted mainly by Na+, Cl−, and K+, were found in the leaf veins and intercellular space near the stoma, indicating that this species has a mechanism of salt exclusion operating on the leaves, which has its role in salt tolerance. The MOI approach showed that 41 metabolites were statistically significant on the leaves and 65 metabolites on the roots of adult purslane plants. The combination of the mummichog algorithm and metabolomics database comparison revealed that the glycine, serine, and threonine, amino sugar and nucleotide sugar, and glycolysis/gluconeogenesis pathways were the most significantly enriched pathways when considering the total number of occurrences in the leaves (with 14, 13, and 13, respectively) and roots (all with eight) of adult plants; and that purslane plants employ the adaptive mechanism of osmoprotection to mitigate the negative effect of very high levels of salinity stress; and that this mechanism is prevalent in the leaves. The multi-omics database built by our group underwent a screen for salt-responsive genes, which are now under further characterization for their potential to promote resistance to salinity stress when heterologously overexpressed in salt-sensitive plants.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%