Multi-Omics Analysis After Vaginal Administration of Bacteroides fragilis in Chickens

Lu, Chun; Yang, Maosen; Zhu, Wei; Su, Yuan; Li, Diyan; Wang, Tao

doi:10.3389/fmicb.2022.846011

Cited by 5 publications

(3 citation statements)

References 66 publications

(71 reference statements)

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“…Moreover, R. intestinalis-derived flagellin has been proved as an effective modulator of inflammatory gut responses [45]. B. fragilis was described as promoting mucosal immune development and reduced inflammation [46][47][48]. For endocrine function, we also have a difference between the three bacteria.…”

Section: Discussionmentioning

confidence: 99%

Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells

et al. 2022

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The gut microbiota contributes to human health and disease; however, the mechanisms by which commensal bacteria interact with the host are still unclear. To date, a number of in vitro systems have been designed to investigate the host–microbe interactions. In most of the intestinal models, the enteroendocrine cells, considered as a potential link between gut bacteria and several human diseases, were missing. In the present study, we have generated a new model by adding enteroendocrine cells (ECC) of L-type (NCI-H716) to the one that we have previously described including enterocytes, mucus, and M cells. After 21 days of culture with the other cells, enteroendocrine-differentiated NCI-H716 cells showed neuropods at their basolateral side and expressed their specific genes encoding proglucagon (GCG) and chromogranin A (CHGA). We showed that this model could be stimulated by commensal bacteria playing a key role in health, Roseburia intestinalis and Bacteroides fragilis, but also by a pathogenic strain such as Salmonella Heidelberg. Moreover, using cell-free supernatants of B. fragilis and R. intestinalis, we have shown that R. intestinalis supernatant induced a significant increase in IL-8 and PYY but not in GCG gene expression, while B. fragilis had no impact. Our data indicated that R. intestinalis produced short chain fatty acids (SCFAs) such as butyrate whereas B. fragilis produced more propionate. However, these SCFAs were probably not the only metabolites implicated in PYY expression since butyrate alone had no effect. In conclusion, our new quadricellular model of gut epithelium could be an effective tool to highlight potential beneficial effects of bacteria or their metabolites, in order to develop new classes of probiotics.

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Section: Discussionmentioning

confidence: 99%

Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells

et al. 2022

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“…The quality and integrity of the extracted DNA were assessed using a NanoDrop 2000 spectrophotometer and 1% agarose gel electrophoresis. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using the forward primer 341F (5 -CCTAYGGGRBGCASCAG-3 ) and reverse primer 806R (5 -GGACTACNNGGGTATCTAAT-3 ) [36]. The PCR reaction system consisted of 15 µL Phusion ® High-Fidelity PCR Master Mix (New England Biolabs, Beijing, China), 0.2 µM forward and reverse primers, and approximately 10 ng of template DNA.…”

Section: Dna Extraction and 16s Rrna Sequencing Analysismentioning

confidence: 99%

Gut Microbial Diversity Analysis of Different Native Chickens and Screening of Chicken-Derived Probiotics

Chen,

Bai,

Wang

et al. 2023

Animals

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The gut microbiota plays a critical role in the growth, development, nutritional digestion, and overall health of chickens. Furthermore, certain probiotics isolated from poultry intestines have demonstrated the potential to enhance immune function and production performance in chickens. To investigate the differences in gut microbiota among chickens from various geographical environments and different breeds of broiler and laying hens, we conducted 16S rRNA sequencing on the fecal microbiota of 140 Chinese native chickens and ten Roman layers. In addition, we isolated and screened the potential probiotics to examine their biological characteristics, genome profiles, and functionality in animals. Our findings revealed the significant variations in gut microbiota composition and structure between Tibetan chickens (ZJ), which reside in high-altitude regions, and Meihua chickens (MH) and Xuhai chickens (XH), which inhabit low-altitude regions. Specifically, Cupriavidus and Candidatus_Bacilloplasma were identified as unique microbial communities in high and low altitudes, respectively. Notably, among regions with similar altitudes, Luning chickens (LN) exhibited the lowest α diversity, accompanied by a remarkably high relative abundance of Firmicutes and Lactobacillus. Conversely, Wugu chickens (WGs) and Yaoshan chickens (YSs) displayed similar gut microbiota profiles. Furthermore, distinctive gut microbiota patterns were observed between the different breeds of broilers and laying hens. Commercial Roman layers (LMs) exhibited significantly lower alpha diversity compared to native chickens, and broilers and laying hens predominantly harbored Firmicutes, Bacteroidota, and Proteobacteria. Of particular interest, the probiotics Lactobacillus agilis MH1 and Lactobacillus salivarius ZJ1, derived from chicken feces, exhibited favorable genomic profiles, and demonstrated anti-colitis effects and immunomodulatory functions. These findings provide a crucial theoretical foundation for native chicken research and offer insights for the future development and formulation of chicken-derived probiotics.

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“…The vaginal microbiota plays an important role in maintaining vaginal health and protecting the host from diseases [ 8 , 9 ]. Vaginal microbiota can be affected by host physiology and can also affect the host reproductive physiology itself [ 8 , 10 ]. There is increasing interest in identifying the microbiota that specifically impacts female reproductive health and the health of offspring.…”

Section: Introductionmentioning

confidence: 99%

Vaginal microbiota are associated with in vitro fertilization during female infertility

Wang,

Li,

Bai

et al. 2024

iMeta

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The vaginal microbiome plays an essential role in the reproductive health of human females. As infertility increases worldwide, understanding the roles that the vaginal microbiome may have in infertility and in vitro fertilization (IVF) treatment outcomes is critical. To determine the vaginal microbiome composition of 1411 individuals (1255 undergoing embryo transplantation) and their associations with reproductive outcomes, clinical and biochemical features are measured, and vaginal samples are 16S rRNA sequenced. Our results suggest that both too high and too low abundance of Lactobacillus is not beneficial for pregnancy; a moderate abundance is more beneficial. A moderate abundance of Lactobacillus crispatus and Lactobacillus iners (~80%) (with a pregnancy rate of I‐B: 54.35% and III‐B: 57.73%) is found beneficial for pregnancy outcomes compared with a higher abundance (>90%) of Lactobacillus (I‐A: 44.81% and III‐A: 51.06%, respectively). The community state type (CST) IV‐B (contains a high to moderate relative abundance of Gardnerella vaginalis) shows a similar pregnant ratio (48.09%) with I‐A and III‐A, and the pregnant women in this CST have a higher abundance of Lactobacillus species. Metagenome analysis of 71 samples shows that nonpregnant women are detected with more antibiotic‐resistance genes, and Proteobacteria and Firmicutes are the main hosts. The inherent differences within and between women in different infertility groups suggest that vaginal microbes might be used to detect infertility and potentially improve IVF outcomes.

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Multi-Omics Analysis After Vaginal Administration of Bacteroides fragilis in Chickens

Cited by 5 publications

References 66 publications

Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells

Roseburia intestinalis Modulates PYY Expression in a New a Multicellular Model including Enteroendocrine Cells

Gut Microbial Diversity Analysis of Different Native Chickens and Screening of Chicken-Derived Probiotics

Vaginal microbiota are associated with in vitro fertilization during female infertility

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