Multi-omics analyses reveal molecular mechanisms for the antagonistic toxicity of carbon nanotubes and ciprofloxacin to Escherichia coli

Deng, Rui; Gao, Xuan; Hou, Jie; Lin, Daohui

doi:10.1016/j.scitotenv.2020.138288

Cited by 32 publications

(15 citation statements)

References 85 publications

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“…Among them, carbon nanotubes are the most popular support for enzyme immobilization owing to their unique characteristics including the layered and hollow structures, low price, high chemical and thermal stabilities, high mechanical strength, high enzyme loading, good biocompatibility, and large surface area to volume ratio. , However, their practical utilization in bioremediation is restricted by their agglomeration capacity as a result of the strong attraction between the small carbon particles, which leads to the smaller surface area . To remove this limitation, carbon nanotubes can be prepared in advance by a surface modification to decrease the agglomeration and increase the accessible surface area. − Therefore, mesoporous silica materials modified carbon nanotubes may be advantageous owing to their quicker mass transfer, enhanced interfacial bonding ability, hydrophilicity property, mechanical stability, and high enzyme loading, and different functional groups can be attached on it, which preclude enzyme leaching. − …”

Section: Introductionmentioning

confidence: 99%

Improvement of Laccase Activity Via Covalent Immobilization over Mesoporous Silica Coated Magnetic Multiwalled Carbon Nanotubes for the Discoloration of Synthetic Dyes

et al. 2021

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Due to its environmental friendliness and biodegradable ability, the enzymatic decolorization of azo dyes is the best option. However, the free enzyme suffers from various limitations, including poor stability, no repeatable use, and a high expense, which is the key drawback for its practical use. In this analysis, the laccase enzyme was immobilized in mesoporous silica coated magnetic multiwalled carbon nanotubes (Fe 3 O 4 -MWCNTs@SiO 2 ) by a glutaraldehyde cross-linker to create an easily separable and stable enzyme. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive Xray spectroscopy (EDX) were used to characterize the as-synthesized Fe 3 O 4 -MWCNTs@SiO 2 . Laccase immobilized in Fe 3 O 4 -MWCNTs@SiO 2 showed a good improvement in temperature, pH, and storage stability. Moreover, the operational stability of the biocatalyst was improved, retaining 87% of its original activity even after 10 cycles of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) oxidation. The biocatalysts were applied for the decolorization of selected azo dyes without a mediator, and up to 99% of Eriochrome Black T (EBT), 98% of Acid Red 88 (AR 88), and 66% of Reactive Black 5 (RB5) were decolorized. Based on these properties, the biocatalysts can be potentially utilized in various environmental and industrial applications.

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Section: Introductionmentioning

confidence: 99%

Improvement of Laccase Activity Via Covalent Immobilization over Mesoporous Silica Coated Magnetic Multiwalled Carbon Nanotubes for the Discoloration of Synthetic Dyes

et al. 2021

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“…This study thus demonstrated that transcriptomics can serve as a sensitive tool in revealing ENM effects that could be useful in fighting antibiotic resistance. On the other hand, there are reports on antagonistic activity of oxidized MWCNTs and organic compounds such as the pesticide pentachlorophenol ( Deng et al, 2019 ) and the antibiotic ciprofloxacin ( Deng et al, 2020 ). Transcriptomics and a combination of transcriptomics and metabolomics, respectively, indicated that MWCNTs attenuated the pentachlorophenol-induced disturbances in gene expression ( Deng et al, 2019 ) and offset the impact of ciprofloxacin in E. coli gene expression and its metabolome ( Deng et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, there are reports on antagonistic activity of oxidized MWCNTs and organic compounds such as the pesticide pentachlorophenol ( Deng et al, 2019 ) and the antibiotic ciprofloxacin ( Deng et al, 2020 ). Transcriptomics and a combination of transcriptomics and metabolomics, respectively, indicated that MWCNTs attenuated the pentachlorophenol-induced disturbances in gene expression ( Deng et al, 2019 ) and offset the impact of ciprofloxacin in E. coli gene expression and its metabolome ( Deng et al, 2020 ). The different outcomes of MWCNT exposures to the toxicity of organic compounds in bacteria may be caused by the physico-chemical properties of these compounds that influence their interactions with MWCNTs and bacteria, or depend on whether pre- or co-exposures of MWCNTs with the antibiotics were conducted.…”

Section: Resultsmentioning

confidence: 99%

Molecular Mechanisms of Nanomaterial-Bacterial Interactions Revealed by Omics—The Role of Nanomaterial Effect Level

Mortimer

Wang

Holden

2021

Front. Bioeng. Biotechnol.

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Nanotechnology is employed across a wide range of antibacterial applications in clinical settings, food, pharmaceutical and textile industries, water treatment and consumer goods. Depending on type and concentration, engineered nanomaterials (ENMs) can also benefit bacteria in myriad contexts including within the human body, in biotechnology, environmental bioremediation, wastewater treatment, and agriculture. However, to realize the full potential of nanotechnology across broad applications, it is necessary to understand conditions and mechanisms of detrimental or beneficial effects of ENMs to bacteria. To study ENM effects, bacterial population growth or viability are commonly assessed. However, such endpoints alone may be insufficiently sensitive to fully probe ENM effects on bacterial physiology. To reveal more thoroughly how bacteria respond to ENMs, molecular-level omics methods such as transcriptomics, proteomics, and metabolomics are required. Because omics methods are increasingly utilized, a body of literature exists from which to synthesize state-of-the-art knowledge. Here we review relevant literature regarding ENM impacts on bacterial cellular pathways obtained by transcriptomic, proteomic, and metabolomic analyses across three growth and viability effect levels: inhibitory, sub-inhibitory or stimulatory. As indicated by our analysis, a wider range of pathways are affected in bacteria at sub-inhibitory vs. inhibitory ENM effect levels, underscoring the importance of ENM exposure concentration in elucidating ENM mechanisms of action and interpreting omics results. In addition, challenges and future research directions of applying omics approaches in studying bacterial-ENM interactions are discussed.

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“…Our research demonstrated that the sea urchin S. intermedius can be used in the testing of NPs. However, we believe that further investigations of NP environmental risk assessment should focus on the incorporation of multiomics approaches, predicting-toxicity tools, and the evaluation of the problems of biocorona formation and biotransformation of NPs by the organisms and in the media [ 55 , 56 , 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Carbon, Silicon, and Metal-Based Nanoparticles to Sea Urchin Strongylocentrotus intermedius

et al. 2020

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With the increasing annual production of nanoparticles (NPs), the risks of their harmful influence on the environment and human health are rising. However, our knowledge about the mechanisms of interaction between NPs and living organisms is limited. Prior studies have shown that echinoderms, and especially sea urchins, represent one of the most suitable models for risk assessment in environmental nanotoxicology. To the best of the authors’ knowledge, the sea urchin Strongylocentrotus intermedius has not been used for testing the toxicity of NPs. The present study was designed to determine the effect of 10 types of common NPs on spermatozoa activity, egg fertilization, and early stage of embryo development of the sea urchin S. intermedius. In this research, we used two types of multiwalled carbon nanotubes (CNT-1 and CNT-2), two types of carbon nanofibers (CNF-1 and CNF-2), two types of silicon nanotubes (SNT-1 and SNT-2), nanocrystals of cadmium and zinc sulfides (CdS and ZnS), gold NPs (Au), and titanium dioxide NPs (TiO2). The results of the embryotoxicity test showed the following trend in the toxicity level of used NPs: Au > SNT-2 > SNT-1 > CdS > ZnS > CNF-2 > CNF-1 > TiO2 > CNT-1 > CNT-2. This research confirmed that the sea urchin S. intermedius can be considered as a sensitive and stable test model in marine nanotoxicology.

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Multi-omics analyses reveal molecular mechanisms for the antagonistic toxicity of carbon nanotubes and ciprofloxacin to Escherichia coli

Cited by 32 publications

References 85 publications

Improvement of Laccase Activity Via Covalent Immobilization over Mesoporous Silica Coated Magnetic Multiwalled Carbon Nanotubes for the Discoloration of Synthetic Dyes

Improvement of Laccase Activity Via Covalent Immobilization over Mesoporous Silica Coated Magnetic Multiwalled Carbon Nanotubes for the Discoloration of Synthetic Dyes

Molecular Mechanisms of Nanomaterial-Bacterial Interactions Revealed by Omics—The Role of Nanomaterial Effect Level

Toxicity of Carbon, Silicon, and Metal-Based Nanoparticles to Sea Urchin Strongylocentrotus intermedius

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