Multi-omic comparisons between CFBE41o- cells stably expressing wild-type CFTR and F508del-mutant CFTR

Lu, Shiping; Kolls, Jay K.

doi:10.1016/j.jcf.2022.06.010

Cited by 3 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 E). A previous study reported the impact of growth conditions of CFBE cells on cytokine release recently [ 52 ]. We also found that the spontaneous release of CLCA1 entirely depends on the growth conditions of CFBE cells, but independent of the expression of CFTR (Fig.…”

Section: Resultsmentioning

confidence: 99%

Niclosamide, but not ivermectin, inhibits anoctamin 1 and 6 and attenuates inflammation of the respiratory tract

Ousingsawat,

Centeio,

Schreiber

et al. 2023

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

Inflammatory airway diseases like cystic fibrosis, asthma and COVID-19 are characterized by high levels of pulmonary cytokines. Two well-established antiparasitic drugs, niclosamide and ivermectin, are intensively discussed for the treatment of viral inflammatory airway infections. Here, we examined these repurposed drugs with respect to their anti-inflammatory effects in airways in vivo and in vitro. Niclosamide reduced mucus content, eosinophilic infiltration and cell death in asthmatic mouse lungs in vivo and inhibited release of interleukins in the two differentiated airway epithelial cell lines CFBE and BCi-NS1.1 in vitro. Cytokine release was also inhibited by the knockdown of the Ca2+-activated Cl− channel anoctamin 1 (ANO1, TMEM16A) and the phospholipid scramblase anoctamin 6 (ANO6, TMEM16F), which have previously been shown to affect intracellular Ca2+ levels near the plasma membrane and to facilitate exocytosis. At concentrations around 200 nM, niclosamide inhibited inflammation, lowered intracellular Ca2+, acidified cytosolic pH and blocked activation of ANO1 and ANO6. It is suggested that niclosamide brings about its anti-inflammatory effects at least in part by inhibiting ANO1 and ANO6, and by lowering intracellular Ca2+ levels. In contrast to niclosamide, 1 µM ivermectin did not exert any of the effects described for niclosamide. The present data suggest niclosamide as an effective anti-inflammatory treatment in CF, asthma, and COVID-19, in addition to its previously reported antiviral effects. It has an advantageous concentration–response relationship and is known to be well tolerated.

show abstract

Section: Resultsmentioning

confidence: 99%

Niclosamide, but not ivermectin, inhibits anoctamin 1 and 6 and attenuates inflammation of the respiratory tract

Ousingsawat,

Centeio,

Schreiber

et al. 2023

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

show abstract

“…Recent studies have demonstrated notable changes in primary cell physiology when cultured in media with different additives [ 23 ]. Differences in the physiological behavior of the CFBE41o- line have been reported when the cultures are grown in submerged versus air-exposed conditions [ 24 ]. To our knowledge, however, no studies have examined how the CFBE41o- cell line’s behavior and physiology are impacted by media selection.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Alteration of an Established Human Airway Cell Line by Media Selection

Livnat

Meeker

Ostmann

et al. 2023

IJMS

View full text Add to dashboard Cite

Cystic Fibrosis (CF) is caused by mutations in the CF transmembrane conductance regulator (CFTR), a chloride/bicarbonate channel. Many studies utilize human airway cell models (cell lines and primary cells) to study different aspects of CFTR biology. Media selection can alter the growth and differentiation of primary cells, yet the impact on stable airway cell lines is unclear. To determine the impact of media and growth conditions on CFBE41o- cells stably transduced with wild-type or F508del CFTR, we examined four commonly used growth media, measuring epithelial and mesenchymal markers, as well as CFTR expression, maturation, and function. The selection of growth media altered the expression of epithelial and mesenchymal markers in the cell lines, and significantly impacted CFTR expression and subsequent function. These results highlight the importance of media selection to CFTR and cell line behavior and should be considered in both studies of primary human airway cells and stable cell lines.

show abstract

How to use an in vitro approach to characterize the toxicity of airborne compounds

Jaber,

Billet

2024

Toxicology in Vitro

View full text Add to dashboard Cite

Multi-omic comparisons between CFBE41o- cells stably expressing wild-type CFTR and F508del-mutant CFTR

Cited by 3 publications

References 28 publications

Niclosamide, but not ivermectin, inhibits anoctamin 1 and 6 and attenuates inflammation of the respiratory tract

Niclosamide, but not ivermectin, inhibits anoctamin 1 and 6 and attenuates inflammation of the respiratory tract

Phenotypic Alteration of an Established Human Airway Cell Line by Media Selection

How to use an in vitro approach to characterize the toxicity of airborne compounds

Contact Info

Product

Resources

About