“…Various methods have been developed to assist passive mixing based on their specific features, briefly listed as: Spiral micromixers [12,13,14], zigzag-shaped channels [15], T-shaped mixers [16], floor-grooved channels [17,18], herringbone mixers [19,20], obstacle-based mixers [21], convergent-divergent walls [22], split and recombine (SAR) mixers [23,24,25,26,27,28,29] and lamination-based mixers [20,30,31]. All of the above-mentioned methods may be used in both planar [12,15,21,22,23,25,28,32] or three-dimensional (3D) [13,14,16,17,18,19,20,24,26,27,29,30,31] systems. 3D passive micromixers typically benefit from spatial structures to generate more effective vortices.…”