Multi-network granular hydrogel with enhanced strength for 3D bioprinting

Wang, Wei; Chen, Xi; Meng, Teng; Liu, Lei

doi:10.1177/08853282221075198

Cited by 11 publications

(10 citation statements)

References 19 publications

(27 reference statements)

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“…[153] Research indicates that augmenting the precursor solution's viscosity can enhance the mechanical properties of 3D-printed hydrogel scaffolds. [154] New high-strength hydrogels, including dual-network hydrogels [155] and ionic crosslinked hydrogels, [156] are emerging. These hydrogels meet the mechanical loading needs of human load-bearing structures, such as knee joints, and surpass the weak mechanical characteristics of conventional hydrogels (Table 3).…”

Section: Application Of Mechanical Reinforcement-based Hydrogel In Me...mentioning

confidence: 99%

Advanced Hydrogel Material for Meniscus Repair

Li,

Deng,

Yang

et al. 2024

Adv Funct Materials

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The foundation of regenerative medicine is the investigation and progression of hypotheses and approaches for substituting, mending, reconstructing, or regenerating different tissues and organs within the human body. Nevertheless, due to the slender central segment and the entire unattached configuration of the meniscus, the efficacy of conventional therapies is unsatisfactory in reinstating meniscal health and functionality. To overcome these limitations, medical‐type hydrogels have the potential to provide a solution for meniscus repair and regeneration. Their biocompatibility and modifiability enable safe implantations in vivo, effectively modifying the pathophysiology of cells and the surrounding microenvironment at the site of meniscal injury. This article reviews the usage of different implantable medical‐type hydrogels in meniscal regeneration and repair. First, the structure and physiologic function of the meniscus are first briefly described. Following this, the use of varying hydrogels in meniscus repair is highlighted, alongside a discussion of different materials employed in constructing hydrogel implants. Lastly, an analysis of the issues and challenges associated with hydrogels in meniscus repair is presented.

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Section: Application Of Mechanical Reinforcement-based Hydrogel In Me...mentioning

confidence: 99%

Advanced Hydrogel Material for Meniscus Repair

Li,

Deng,

Yang

et al. 2024

Adv Funct Materials

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“…[223] Wang et al combined GelMA with granular hydrogel composed of hydroxypropyl chitosan microspheres to form a compound bioink containing adipose derived stem cells (ASCs). [224] The bioink was then 3D bioprinted to make cylindrical rings. The granular hydrogel showed superior storage modulus, self-healing ability, and significantly higher proliferation of ASCs.…”

Section: Gelatin-chitosanmentioning

confidence: 99%

Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Asim

Tabish

Liaqat

et al. 2023

Adv Healthcare Materials

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Gelatin is a widely utilized bioprinting biomaterial due to its cell‐adhesive and enzymatically cleavable properties, which improve cell adhesion and growth. Gelatin is often covalently cross‐linked to stabilize bioprinted structures, yet the covalently cross‐linked matrix is unable to recapitulate the dynamic microenvironment of the natural extracellular matrix (ECM), thereby limiting the functions of bioprinted cells. To some extent, a double network bioink can provide a more ECM‐mimetic, bioprinted niche for cell growth. More recently, gelatin matrices are being designed using reversible cross‐linking methods that can emulate the dynamic mechanical properties of the ECM. This review analyzes the progress in developing gelatin bioink formulations for 3D cell culture, and critically analyzes the bioprinting and cross‐linking techniques, with a focus on strategies to optimize the functions of bioprinted cells. This review discusses new cross‐linking chemistries that recapitulate the viscoelastic, stress‐relaxing microenvironment of the ECM, and enable advanced cell functions, yet are less explored in engineering the gelatin bioink. Finally, this work presents the perspective on the areas of future research and argues that the next generation of gelatin bioinks should be designed by considering cell–matrix interactions, and bioprinted constructs should be validated against currently established 3D cell culture standards to achieve improved therapeutic outcomes.

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“…However, a different approach employed a different methodology by preparing a multinetwork granular hydrogel for extrusion 3D printing. [ 73 ] In this case, spherical microgels of poly ( γ ‐glutamic acid) (PG) and hydroxy propyl chitosan (CSPO) were prepared by batch emulsion and dispersed to a solution of gelatin methacrylate (GelMA) instead of a simple aqueous solution. Even though the microgels were present in a GelMA solution, the material showed shear‐thinning and self‐healing properties.…”

Section: Considerations For Granular Inks: Formulation and Challengesmentioning

confidence: 99%

Programmable Granular Hydrogel Inks for 3D Bioprinting Applications

Ribeiro

Gaspar

Sobreiro‐Almeida

et al. 2023

Adv Materials Technologies

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Granular inks comprising jammed hydrogel unit building blocks are emerging as multiprogramable precursors for 3D/4D printing nonbulk hydrogel constructs. In addition to their injectability, they also exhibit high porosity when compared to bulk hydrogels, allowing more efficient nutrient transport and cell migration through the scaffold structure. Herein, the key steps in the production of these inks, from the fabrication of the microgels, the jamming process, and how fabrication affects final material properties, such as porosity, resolution, and fidelity is reviewed. In addition, the main techniques used for the stabilization of scaffolds after the printing process and the assessment of cell viability, in the case of bioinks, are meticulously discussed. Finally, the most recent studies in the application of granular hydrogels for different biomedical applications are highlighted. All in all, it is envisioned that 3D‐printed granular constructs will continue to evolve towards increasingly stimuli‐responsive platforms that may respond in a spatiotemporally controlled manner that matches that of user‐defined or biologically encoded processes.

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Multi-network granular hydrogel with enhanced strength for 3D bioprinting

Cited by 11 publications

References 19 publications

Advanced Hydrogel Material for Meniscus Repair

Advanced Hydrogel Material for Meniscus Repair

Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Programmable Granular Hydrogel Inks for 3D Bioprinting Applications

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