Multi-modal nonlinear optical and thermal imaging platform for label-free characterization of biological tissue

Adams, Wilson R.; Mehl, Brian P.; Lieser, Eric; Wang, Manqing; Patton, Shane; Throckmorton, Graham A.; Jenkins, J. Logan; Ford, Jeremy B.; Gautam, Rekha; Brooker, Jeff Z.; Jansen, E.; Mahadevan‐Jansen, Anita

doi:10.1038/s41598-021-86774-2

Cited by 14 publications

(14 citation statements)

References 39 publications

(42 reference statements)

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“…collagen, myosin thick filaments, microtubules) [55]. Such methods use near-IR excitation, penetrate deeper into tissues, and can be multiplexed to enable imaging of cells and tissues with prominent applications in cancer detection [56][57][58]. The concepts and algorithms reported for miPolScope can be extended to multiphoton imaging techniques to provide a more complete view of tissue morphology and pathology.…”

Section: Discussionmentioning

confidence: 99%

Correlative imaging of spatio-angular dynamics of molecular assemblies and cells with multimodal instant polarization microscope

Ivanov

Yeh

Pérez-Bermejo

et al. 2022

Preprint

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Biological function depends on the spatio-angular architecture of macromolecules - for example, functions of lipid membrane and cytoskeletal polymers arise from both the spatial and the angular organization of the constituent molecules. Correlative imaging of cellular and molecular architecture is valuable across cell biology and pathology. However, current live imaging methods primarily focus on spatial component of the architecture. Imaging the dynamic angular architecture of cells and organelles requires fast polarization-, depth-, and wavelength-diverse measurement of intrinsic optical properties and fluorophore concentration, but remains challenging with current designs. We report a multimodal instant polarization microscope (miPolScope) that combines a broadband polarization-resolved detector, automation, and reconstruction algorithms to enable label-free imaging of phase, retardance, and orientation, multiplexed with fluorescence imaging of concentration, anisotropy, and orientation of molecules at diffraction-limited resolution and high speed. miPolScope enabled multimodal imaging of myofibril architecture and contractile activity of beating cardiomyocytes, cell and organelle architecture of live HEK293T and U2OS cells, and density and anisotropy of white and grey matter of mouse brain tissue across the visible spectrum. We anticipate these developments in joint quantitative imaging of density and anisotropy to enable new studies in tissue pathology, mechanobiology, and imaging-based screens.

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Section: Discussionmentioning

confidence: 99%

Correlative imaging of spatio-angular dynamics of molecular assemblies and cells with multimodal instant polarization microscope

Ivanov

Yeh

Pérez-Bermejo

et al. 2022

Preprint

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“…CARS imaging was performed using a custom-built multimodal imaging platform that was designed and calibrated as described earlier. 42 Cells were seeded on uncoated glass bottom petri dishes (1.5 Coverslip, 35 mm diameter, Mattek, USA) 48 hours before CARS imaging. The cells were subsequently imaged on the epi-detection port, which is equipped with a photomultiplier tube (GaAsP Amplified PMT, Thorlabs, USA).…”

Section: Methodsmentioning

confidence: 99%

Enhanced characterization of breast cancer phenotypes using Raman micro-spectroscopy on stainless steel substrate

Thomas

Fitzgerald

Gautam³

et al. 2023

Anal. Methods

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“…Notably, this intensity is approximately 4 or more orders of magnitude lower than typical NLO bioimaging with high numerical aperture objectives. 28,29 By comparing the TPF images in Figure 2b and 2d, one can clearly notice that some cells already have died during the CARS image acquisition. This photodamage of the live cells is manifested by the appearance of more red spots in the images shown in Figure 2d than those seen in Figure 2b.…”

Section: ■ Photobleaching and Photodegradationmentioning

confidence: 96%

Nonlinear Optical Microscopy with Ultralow Quantum Light

Doughty

2021

J. Phys. Chem. A

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Nonlinear optical (NLO) microscopy relies on multiple light−matter interactions to provide unique contrast mechanisms and imaging capabilities that are inaccessible to traditional linear optical imaging approaches, making them versatile tools to understand a wide range of complex systems. However, the strong excitation fields that are necessary to drive higher-order optical processes efficiently are often responsible for photobleaching, photodegradation, and interruption in many systems of interest. This is especially true for imaging living biological samples over prolonged periods of time or in accessing intrinsic dynamics of electronic excited-state processes in spatially heterogeneous materials. This perspective outlines some of the key limitations of two NLO imaging modalities implemented in our lab and highlights the unique potential afforded by the quantum properties of light, especially entangled two-photon absorption based NLO spectroscopy and microscopy. We further review some of the recent exciting advances in this emerging filed and highlight some major challenges facing the realization of quantum-light-enabled NLO imaging modalities.

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Multi-modal nonlinear optical and thermal imaging platform for label-free characterization of biological tissue

Cited by 14 publications

References 39 publications

Correlative imaging of spatio-angular dynamics of molecular assemblies and cells with multimodal instant polarization microscope

Correlative imaging of spatio-angular dynamics of molecular assemblies and cells with multimodal instant polarization microscope

Enhanced characterization of breast cancer phenotypes using Raman micro-spectroscopy on stainless steel substrate

Nonlinear Optical Microscopy with Ultralow Quantum Light

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