Multi-method for the determination of antibiotics of different substance groups in milk and validation in accordance with Commission Decision 2002/657/EC

Bohm, Detlef A.; Stachel, Carolin S.; Gowik, Petra

doi:10.1016/j.chroma.2009.06.058

Cited by 81 publications

(43 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(Bohm et al 2009;Chico et al 2008;Dasenaki and Thomaidis 2015;Freitas et al 2014;Frenich et al 2010;Geis-Asteggiante et al 2012;Granelli and Branzell 2007;Han et al 2015;Hermo et al 2008;Kaufmann et al 2014;Martins-Júnior et al 2007;Mastovska and Lightfield 2008;Ortelli et al 2009;Stolker et al 2008;Turnipseed et al 2014;Wang et al 2015) but there is a lack in the method development for processed dairy products, since antibiotics are not fully degraded during pasteurization process (Pérez et al 2013).…”

Section: Additionally This Technique Fulfills the Requirements Demmentioning

confidence: 99%

Development and Validation of a Multi-class UHPLC-MS/MS Method for Determination of Antibiotic Residues in Dairy Products

2017

View full text Add to dashboard Cite

In regard to the knowledge of provoking a worldwide resistance against antibiotics due to incorrect application and the resulting uptake of residues via the food chain, the European Union set maximum residue levels (MRLs) for these substances in animal derived products to protect the customers. In respect of these MRLs a multi-class UHPLC-MS/MS multiclass method has been developed for the simultaneous determination of 30 substances from different compound groups (quinolones, macrolides, lincosamides, β-lactams, sulphonamides, diamino-pyrimidine derivates and tetracyclines) in various kinds of dairy products. Since this method should be suitable for routine laboratory use, sample preparation requires an easy and fast approach to ensure high sample throughput. Therefore a sample preparation with C18EC dSPE bulk sorbent as a combination of the easy and quick sample preparation provided by QuEChERS and the clean-up principle of reversed phase SPE was applied. This method was validated for all compounds in all matrices in compliance with the requirements of Commission Decision 2002/657/EC.

show abstract

Section: Additionally This Technique Fulfills the Requirements Demmentioning

confidence: 99%

Development and Validation of a Multi-class UHPLC-MS/MS Method for Determination of Antibiotic Residues in Dairy Products

2017

View full text Add to dashboard Cite

show abstract

“…A small number of published methods were reported for the simultaneous analysis of multiple drug classes. Liquid (modified with acid) -based or organic solvent-based (MeOH, MeCN) extraction [11][12][13], solid-phase extraction (SPE) [14,15], QuEChERS (quick, easy, cheap, effective, rugged and safe) approach [16] and pressurized liquid extraction (PLE) [17] were also employed for sample preparation of multiple drug residues. Yamada et al [18] highlighted an extensive method for the determination of 130 drugs from multiple classes, except aminoglycosides and tetracyclines.…”

Section: Introductionmentioning

confidence: 99%

Efficient hydrophilic interaction liquid chromatography–tandem mass spectrometry for the multiclass analysis of veterinary drugs in chicken muscle

Chiaochan

Koesukwiwat

Yudthavorasit

et al. 2010

Analytica Chimica Acta

View full text Add to dashboard Cite

“…In letteratura sono descritti diversi metodi per la ricerca di residui di chinoloni nel latte; le tecniche microbiologiche non hanno sensibilità adeguata a soddisfare i requisiti richiesti dalla normativa vigente; le tecniche immunochimiche (Haasnoot et al, 2007;Wang et al, 2010), la cromatografia su strato sottile (TLC) (Chroma et al, 1999;Juhel-Gaugain e Abejan, 1998), la gas cromatografia (GC) e la cromatografia liquida ad alte prestazioni (HPLC) accoppiata con rivelatore spettrofluorimetrico (FLD), a sequenza di diodi (DAD) o a spettrometria di massa (MS) (Gigosos et al, 2000;Yorke e Froc, 2000;Ramos et al, 2003;Ho et al, 2004;Marazuela e Moreno-Bondi, 2004;Van Hoof et al, 2005;Zhao et al, 2007;Bohm et al, 2009;Zhang et al, 2009) sono invece maggiormente sensibili e in taluni casi idonee a una inequivocabile identificazione degli analiti. Tuttavia questi metodi di solito comprendono solo una delle sottoclassi di chinoloni (derivati dell'acido piridoncarbossilico o fluorochinoloni con sostituente piperazinile al C-7).…”

Section: Introduzioneunclassified

Multiresidue confirmatory method for determination of quinolones in milk by HPLC: method development and validation according to the criteria of Commission Decision 2002/657/EC

et al. 2013

View full text Add to dashboard Cite

Veterinary drugs have become an integral part of the livestock production and play an important role in maintaining animal welfare. The use of veterinary medicines may be cause of the presence of drug residues in animal food products if appropriate withdrawal periods are not respected or if contaminated feeds are used. This work presents the development of an high performance liquid chromatography with postcolumn fluorescence derivatization (HPLC-FLD) method for the quantitative detection of eight quinolones -norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, difloxacin, oxolinic acid, nalidixic acid, and flumequine -in bovine milk. After deproteination and extraction with a metaphosphoric acid 1% w/v/methanol/acetonitrile (60/20/20 v/v/v) solution, the sample is partially evaporated and cleaned up on a reversed phase solid phase extraction (SPE) cartridge. The extract is analyzed using an HPLC-FLD. Mean recovery ranged between 65-88%. The method is validated as a confirmatory method according to Decision 2002/657/EC. All the verified parameters (linearity, selectivity/specificity, trueness, precision, CC , CC , ruggedness and stability) were satisfactory and the method is able to quantify all the analytes in milk in the concentration range 15-60 µg/Kg for danofloxacin and 25-150 µg/Kg for the other quinolones. IntroduzioneI chinoloni sono chemioterapici antibatterici di sintesi caratterizzati da ampio spettro d'azione, in quanto esplicano la loro attività nei confronti di batteri Gram positivi e Gram negativi (Christian, 1996;Dougherty et al., 2001); sono composti caratterizzati da una struttura chimica comune consistente in un eterociclo aromatico con anelli condensati, un gruppo chetonico in posizione 4, una funzione carbossilica in posizione 3 e un sostituente in posizione 1 costituito da etile, ciclo propile o anello condensato (Figura 1). I chinoloni sono autorizzati in medicina veterinaria per il trattamento delle infezioni urinarie, della diarrea da salmonella e shigella, delle infezioni respiratorie da Haemophilus, streptococcus e pseudomonas. Sono somministrati come forma farmaceutica o in mangimi medicati e acqua di abbeverata.Un utilizzo massivo, improprio o abusivo di antibiotici in zootecnica è causa sia di sviluppo di ceppi batterici resistenti e trasmissibili all'uomo attraverso gli alimenti, sia di presenza residuale di farmaci negli alimenti di origine animale. Il regolamento 470 della Commissione Europea (2009) definisce come residui di farmaci tutte le sostanze farmacologicamente attive, espresse in mg/Kg o µg/Kg, e i loro metaboliti che rimangono negli alimenti ottenuti da animali. L'impiego di farmaci negli animali da reddito deve avvenire in modo da garantire che, al momento del consumo, l'alimento non contenga residui in quantità nociva per il consumatore. L'impatto sulla sicurezza alimentare è pertanto tale da aver indotto l'Unione Europea (UE) a stabilire precise regole in merito all'autorizzazione all'utilizzo dei farmaci negli animali in produzione e al controllo uffici...

show abstract

Multi-method for the determination of antibiotics of different substance groups in milk and validation in accordance with Commission Decision 2002/657/EC

Cited by 81 publications

References 19 publications

Development and Validation of a Multi-class UHPLC-MS/MS Method for Determination of Antibiotic Residues in Dairy Products

Development and Validation of a Multi-class UHPLC-MS/MS Method for Determination of Antibiotic Residues in Dairy Products

Efficient hydrophilic interaction liquid chromatography–tandem mass spectrometry for the multiclass analysis of veterinary drugs in chicken muscle

Multiresidue confirmatory method for determination of quinolones in milk by HPLC: method development and validation according to the criteria of Commission Decision 2002/657/EC

Contact Info

Product

Resources

About