Multi-marker profiling of extracellular vesicles using streaming current and sequential electrostatic labeling

Sahu, Siddharth S.; Gevari, Moein Talebian; Nagy, Ábel; Gestin, Maxime; Hååg, Petra; Lewensohn, Rolf; Viktorsson, Kristina; Karlström, Amelie Eriksson; Dev, Apurba

doi:10.1016/j.bios.2023.115142

Cited by 5 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These observations informed the sequence of labeling steps when preparing samples for NP-SIMS analysis and underscored the need to carefully examine labeling protocols. We note that considerations of steric hindrance are not unique to our sample preparation and EV analysis, and have been reported previously by others. , Additional reports described the need to consider labeling sequencing during multiplexed detection of EV surface markers. , …”

Section: Resultssupporting

confidence: 64%

See 1 more Smart Citation

Nanoprojectile Secondary Ion Mass Spectrometry Enables Multiplexed Analysis of Individual Hepatic Extracellular Vesicles

Lee,

Verkhoturov,

Eller

et al. 2023

ACS Nano

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 64%

“…46,47 Additional reports described the need to consider labeling sequencing during multiplexed detection of EV surface markers. 48,49 Detecting Individual EVs Using NP-SIMS. Having optimized EV labeling and confirmed the specificity of EVtag interactions with SPR, we proceeded to analyze the chemical composition of tagged EVs with NP-SIMS.…”

Section: Resultsmentioning

confidence: 99%

Nanoprojectile Secondary Ion Mass Spectrometry Enables Multiplexed Analysis of Individual Hepatic Extracellular Vesicles

Lee,

Verkhoturov,

Eller

et al. 2023

ACS Nano

View full text Add to dashboard Cite

“…As mentioned, the detection method has been widely studied [11, 17, 23] and improved by many research groups. In addition, the diagnostic opportunities of the method has also been explored, e.g., monitoring the efficacy of precision cancer medicine [11], albeit in a laboratory setting.…”

Section: Discussionmentioning

confidence: 99%

“…The applied sensing principle is described in our previous articles [5, 11, 17]. In brief, a PBS buffer was pushed through the microchannels under hydraulic pressure to generate streaming current.…”

Section: Methodsmentioning

confidence: 99%

Design and optimization of silicon-based electrokinetic microchip for sensitive detection of small extracellular vesicles

Gevari

Sahu

Mitra

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

In this study, we demonstrate the fabrication, characterization, and application of a microchip-based electrokinetic biosensor which exploits streaming current for signal transduction. The sensor chips, fabricated using standard silicon fabrication techniques, are based on Si-glass microfluidics offering precision, robustness, and transparency. A custom-built chip-manifold allowing easy interfacing with standard microfluidic connections is also developed which shows leak-free integration of the microchips up to 6 bar of applied pressure. Within this range of pressure, the devices show linear and highly reproducible values for flow rates and streaming current with RMS noise below 20 pA. The microchips designed for multiplexed measurements were tested with the detection of free proteins (streptavidin) and also transmembrane proteins of small extracellular vesicles (sEVs) to demonstrate the capacity of the microchips to detect various types of bio-analytes. The limit of detection (LOD) for streptavidin was estimated to be 0.5 nM while for the transmembrane protein (CD9), the LOD was found to be 1.2e6 sEVs/mL. The sensitivity (LOD) of the devices was found to be about 4 times better in targeting CD9 transmembrane protein of H1975 extracellular vesicles when compared to commercial silica capillary which was used previously. The improvement in LOD is attributed to the higher surface quality of the sensor in terms of the density of surface charges which may be further exploited for even lower LOD. In addition, optical detection of fluorophore-tagged standard proteins was done through the optical window of the chip manifold and the transparent glass cap of the microchip.

show abstract

“…Deposition of nano- or microscale particles on solid surfaces is of great importance in a variety of fields of science and technology. − Particle-covered surfaces are used, for example, as functional materials in electro-optical devices, − biosensors, − biomaterials, , and plasmon-resonance spectroscopy devices . In other applications (e.g., medical devices and membrane filtration systems), particle deposition needs to be prevented to avoid surface fouling …”

Section: Introductionmentioning

confidence: 99%

Streaming Current for Surfaces Covered by Square and Hexagonal Monolayers of Spherical Particles

Blawzdziewicz,

Adamczyk,

Ekiel-Jeżewska

2023

ACS Omega

View full text Add to dashboard Cite

The interface and particle contributions to the streaming current of flat substrates covered with ordered square or hexagonal monolayers of spherical particles were theoretically evaluated for particle coverage up to close packing. The exact numerical results were approximated using fitting functions that contain exponential and linear terms to account for hydrodynamic screening and charge convection from the particle surfaces exposed to external flow. According to our calculations, the streaming currents for the ordered and random particle arrangements differ within a typical experimental error. Thus, streaming-current measurements, supplemented with our fitting functions, can be conveniently used to evaluate the particle coverage without detailed knowledge of the particle distribution. Our results for equal interface and particle ζ-potentials indicate that roughness can reduce the streaming current by more than 30%, even in the limit of the small size of spherical roughness asperities.

show abstract

Multi-marker profiling of extracellular vesicles using streaming current and sequential electrostatic labeling

Cited by 5 publications

References 30 publications

Nanoprojectile Secondary Ion Mass Spectrometry Enables Multiplexed Analysis of Individual Hepatic Extracellular Vesicles

Nanoprojectile Secondary Ion Mass Spectrometry Enables Multiplexed Analysis of Individual Hepatic Extracellular Vesicles

Design and optimization of silicon-based electrokinetic microchip for sensitive detection of small extracellular vesicles

Streaming Current for Surfaces Covered by Square and Hexagonal Monolayers of Spherical Particles

Contact Info

Product

Resources

About