Multi-level Modulation of Light Signaling by GIGANTEA Regulates Both the Output and Pace of the Circadian Clock

Nohales, María A.; Liu, Wanlu; Duffy, Tomás; Nozue, Kazunari; Sawa, Mariko; Pruneda‐Paz, Jose L.; Maloof, Julin; Jacobsen, Steven E.; Kay, Steve A.

doi:10.1016/j.devcel.2019.04.030

Cited by 61 publications

(112 citation statements)

References 84 publications

Supporting

Mentioning

109

Contrasting

Order By: Relevance

“…Intersection of common pathways between memory and grafting outcomes include phytohormone signal transduction, RNA processing, and circadian clock effects 16 , consistent with sharing an msh1 lineage. Assessment of shared candidate loci targeted for methylation repatterning in the msh1 mutant, memory, and graft progeny datasets included, e.g., ARF7 , involved in lateral root formation 53 , BIG , involved in polar auxin transport 54 , and GIGANTEA , a central regulator of the circadian clock network 55 (Fig. 7 and Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

et al. 2020

View full text Add to dashboard Cite

Plants transmit signals long distances, as evidenced in grafting experiments that create distinct rootstock-scion junctions. Noncoding small RNA is a signaling molecule that is graft transmissible, participating in RNA-directed DNA methylation; but the meiotic transmissibility of graft-mediated epigenetic changes remains unclear. Here, we exploit the MSH1 system in Arabidopsis and tomato to introduce rootstock epigenetic variation to grafting experiments. Introducing mutations dcl2, dcl3 and dcl4 to the msh1 rootstock disrupts siRNA production and reveals RdDM targets of methylation repatterning. Progeny from grafting experiments show enhanced growth vigor relative to controls. This heritable enhancement-through-grafting phenotype is RdDM-dependent, involving 1380 differentially methylated genes, many within auxin-related gene pathways. Growth vigor is associated with robust root growth of msh1 graft progeny, a phenotype associated with auxin transport based on inhibitor assays. Large-scale field experiments show msh1 grafting effects on tomato plant performance, heritable over five generations, demonstrating the agricultural potential of epigenetic variation.

show abstract

Section: Discussionmentioning

confidence: 99%

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Light also causes daytime expression of the LHY-like Myb transcription factor REVEILLE 8 (RVE8) and the LNKs, which activate several evening-expressed clock genes (Farinas & Mas, 2011;Rawat et al, 2011;Hsu et al, 2013;Rugnone et al, 2013;Xie et al, 2014). Recently, GI was shown to influence light signaling via direct functional interaction with PIF proteins, further tightening regulatory control of light on clock-mediated transcription (Nohales et al, 2019). Thus, the integration of light signals is mediated through several points in the circadian clock, namely through the activation of gene expression, degradation of proteins, or PhyB light and temperature-dependent function.…”

Section: Introductionmentioning

confidence: 99%

Changes in ambient temperature are the prevailing cue in determining Brachypodium distachyon diurnal gene regulation

MacKinnon

Cole

et al. 2020

New Phytologist

Self Cite

View full text Add to dashboard Cite

Summary Plants are continuously exposed to diurnal fluctuations in light and temperature, and spontaneous changes in their physical or biotic environment. The circadian clock coordinates regulation of gene expression with a 24 h period, enabling the anticipation of these events. We used RNA sequencing to characterize the Brachypodium distachyon transcriptome under light and temperature cycles, as well as under constant conditions. Approximately 3% of the transcriptome was regulated by the circadian clock, a smaller proportion than reported in most other species. For most transcripts that were rhythmic under all conditions, including many known clock genes, the period of gene expression lengthened from 24 to 27 h in the absence of external cues. To functionally characterize the cyclic transcriptome in B. distachyon, we used Gene Ontology enrichment analysis, and found several terms significantly associated with peak expression at particular times of the day. Furthermore, we identified sequence motifs enriched in the promoters of similarly phased genes, some potentially associated with transcription factors. When considering the overlap in rhythmic gene expression and specific pathway behavior, thermocycles was the prevailing cue that controlled diurnal gene regulation. Taken together, our characterization of the rhythmic B. distachyon transcriptome represents a foundational resource with implications in other grass species.

show abstract

“…This result suggests that Ws and Af can be regarded as suitable materials for further studying photoperiodic flowering regulations. www.nature.com/scientificreports www.nature.com/scientificreports/ According to the carrot genome 48 , previously reported de novo transcriptome genes 42 could be localized to specific chromosomes and some new photoperiod genes were identified in this study, including 9 DcCOLs, 1 DcFT, and 1 DcSOC1, but there is no complete structure of CO 6,11,[15][16][17][18] (Supplementary Table S2). GI plays a general role in controlling circadian rhythms for flowering and is highly conserved in seed plants 16,19,54 .…”

Section: Discussionmentioning

confidence: 83%

“…This progress in the model plant Arabidopsis provides an important reference for researching floral molecular mechanisms in other crops 11,12 . In fact, there are usually more than two or three interconnected pathways involved in regulating flowering under natural conditions 3,12-14 . In the photoperiod pathway, the functional hierarchy GIGANTEA (GI)-CONSTANS (CO)-FT has been identified, and plays a key role in regulating flowering 7,11,[15][16][17][18] . During the late afternoons of long days (LDs), GI forms a complex with the FLAVIN-BINDING, KELCH REPEAT, F BOX 1 (FKF1) to degrade CYCLING DOF FACTORs (CDFs), which contains the major regulators of CO transcription, and consequently, CO protein accumulates to promote flowering by activating the transcription of FT and TWIN SISTER OF FT (TSF) in the leaf vasculature 5,7,8,[19][20][21] .…”

mentioning

confidence: 99%

The Response of COL and FT Homologues to Photoperiodic Regulation in Carrot (Daucus carota L.)

Liu

Chen

et al. 2020

Sci Rep

View full text Add to dashboard Cite

carrot (Daucus carota L.) is a biennial plant requiring vernalization to induce flowering, but long days can promote its premature bolting and flowering. The basic genetic network controlling the flowering time has been constructed for carrot, but there is limited information on the molecular mechanisms underlying the photoperiodic flowering response. The published carrot genome could provide an effective tool for systematically retrieving the key integrator genes of GIGANTEA (GI), CONSTANS-LIKE (COL), FLOWERING LOCUS T (FT), and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) homologues in the photoperiod pathway. In this study, the bolting time of wild species "Songzi" (Ws) could be regulated by different photoperiods, but the orange cultivar "Amsterdam forcing" (Af) displayed no bolting phenomenon. According to the carrot genome and previous de novo transcriptome, 1 DcGI, 15 DcCOLs, 2 DcFTs, and 3 DcSOC1s were identified in the photoperiod pathway. The circadian rhythm peaks of DcGI, DcCOL2, DcCOL5a, and DcCOL13b could be delayed under long days (LDs). The peak value of DcCOL2 in Af (12.9) was significantly higher than that in Ws (6.8) under short day (SD) conditions, and was reduced under LD conditions (5.0). The peak values of DcCOL5a in Ws were constantly higher than those in Af under the photoperiod treatments. The expression levels of DcFT1 in Ws (463.0) were significantly upregulated under LD conditions compared with those in Af (1.4). These responses of DcCOL2, DcCOL5a, and DcFT1 might be related to the different bolting responses of Ws and Af. This study could provide valuable insights into understanding the key integrator genes in the carrot photoperiod pathway.

show abstract

Multi-level Modulation of Light Signaling by GIGANTEA Regulates Both the Output and Pace of the Circadian Clock

Cited by 61 publications

References 84 publications

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

Changes in ambient temperature are the prevailing cue in determining Brachypodium distachyon diurnal gene regulation

The Response of COL and FT Homologues to Photoperiodic Regulation in Carrot (Daucus carota L.)

Contact Info

Product

Resources

About