Multi‐layered inhibition of <i>Streptomyces</i> development: BldO is a dedicated repressor of <i>whiB</i>

Bush, Matthew J.; Chandra, Govind; Findlay, Kim; Buttner, Mark J.

doi:10.1111/mmi.13663

Cited by 17 publications

(30 citation statements)

References 26 publications

(73 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, overexpression of WhiB2 leads to hyperseptation (Gomez and Bishai, ). Similar observations have been made in Streptomyces , in which overexpression of WhiB leads to hypersporulation (Bush et al , ). Collectively, these findings suggest that the WhiB‐orthologues in Mycobacteria also function to regulate cell division.…”

Section: Class II (Whib2/whib/whcd)supporting

confidence: 83%

“…low nutrient availability, heat, oxidative stress) (Geiman et al , ; Lee et al , ) and, in both Mycobacteria and Streptomyces, transcription of whiB7/wblC has been shown to be activated by translation‐inhibiting antibiotics (Morris et al , ; Yoo et al , ). In Streptomyces , the expression of developmental genes, including whiB , whiD and wblA , is controlled as part of a complex regulatory network (Flärdh and Buttner, ; Bush et al , ; ). The overall effect of such control is that few Wbl paralogues are likely to be present at the same time in the cell.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The actinobacterial WhiB‐like (Wbl) family of transcription factors

Bush

2018

Molecular Microbiology

Self Cite

View full text Add to dashboard Cite

SummaryThe WhiB‐like (Wbl) family of proteins are exclusively found in Actinobacteria. Wbls have been shown to play key roles in virulence and antibiotic resistance in Mycobacteria and Corynebacteria, reflecting their importance during infection by the human pathogens Mycobacterium tuberculosis, Mycobacterium leprae and Corynebacterium diphtheriae. In the antibiotic‐producing Streptomyces, several Wbls have important roles in the regulation of morphological differentiation, including WhiB, a protein that controls the initiation of sporulation septation and the founding member of the Wbl family. In recent years, genome sequencing has revealed the prevalence of Wbl paralogues in species throughout the Actinobacteria. Wbl proteins are small (generally ~80–140 residues) and each contains four invariant cysteine residues that bind an O2‐ and NO‐sensitive [4Fe–4S] cluster, raising the question as to how they can maintain distinct cellular functions within a given species. Despite their discovery over 25 years ago, the Wbl protein family has largely remained enigmatic. Here I summarise recent research in Mycobacteria, Corynebacteria and Streptomyces that sheds light on the biochemical function of Wbls as transcription factors and as potential sensors of O2 and NO. I suggest that Wbl evolution has created diversity in protein–protein interactions, [4Fe–4S] cluster‐sensitivity and the ability to bind DNA.

show abstract

Section: Class II (Whib2/whib/whcd)supporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

The actinobacterial WhiB‐like (Wbl) family of transcription factors

Bush

2018

Molecular Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bush et al . () now find that bldO mutants lack an aerial mycelium due to a hypersporulation phenotype (Fig. ).…”

Section: Bldo and The Nature Of Baldnessmentioning

confidence: 57%

“…Following from recent papers of this type, the study by Bush et al . () describes the discovery of an intriguing regulatory layer involved in fine‐tuning the initiation of sporulation. The study also highlights early suppression of the sporulation pathway as a necessity for streptomycetes in order for them to grow vegetatively as a branching mycelium.…”

Section: Streptomyces Venezuelaementioning

confidence: 99%

See 1 more Smart Citation

The Streptomyces O‐B one connection: a force within layered repression of a key developmental decision

Flärdh

McCormick

2017

Molecular Microbiology

View full text Add to dashboard Cite

Summary The study of Streptomyces development has made significant advances in the past few years and ongoing work is poised to add even more. One key to advancing the field has been the application of genome‐wide approaches using Streptomyces venezuelae, which is capable of fairly synchronous sporulation in submerged growth conditions. WhiA and WhiB are well‐known transcriptional regulators governing the pathway for spore formation in aerial hyphae. Recent ChIP‐seq and RNA expression analyses indicated that WhiA and WhiB regulate the same set of genes, each being dependent on the presence of the other to exert control. Functional WhiAB is believed to form when developmental accumulation of WhiB joins constitutive accumulation of WhiA, suggesting that an important developmental decision is the control of WhiB accumulation. Now, a new WhiAB‐controlled gene called bldO has been described and characterized. Strikingly, BldO has one target for repression in the entire genome, whiB. BldO now joins pleiotropic repressor BldD to exert a multi‐layer control of the temporal and spatial activity of WhiB. BldD activity is controlled by c‐di‐GMP concentration and BldO potentially responds to an unknown signal. Together BldO and BldD repress developmental genes from being expressed until the appropriate time.

show abstract

Streptomycete Spores

Elliot¹,

Flärdh²

2020

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

The Gram‐positive Streptomyces bacteria are major inhabitants of soil, and, unusually for bacteria, they have a complex, multicellular life cycle that looks remarkably fungal‐like. Vegetative growth involves the extension of multicellular, branching hyphae, while reproductive growth involves the emergence of nonbranching hyphae into the air, away from the branching vegetative hyphal mass. These aerial hyphae are converted into chains of prespore compartments by coordinated cell division and chromosome segregation. Prespores then mature into thick‐walled spores that have condensed nucleoids, low metabolic activity and are primed for dispersal and survival. Aerial hyphae and spore surfaces are coated with a hydrophobic, proteinaceous fibrous sheath that aids in aerial growth and may help to promote subsequent spore dispersal and interaction with various interfaces. When exposed to appropriate environmental conditions, spores re‐establish metabolic activity, initiate germination and begin the vegetative phase of their life cycle anew. Key Concepts Streptomyces spores are distinctly different from bacterial endospores and may be regarded as a type of exospore. Spores develop from dedicated reproductive structures called aerial hyphae. Streptomyces spores are formed by partitioning long, multigenomic hyphal cells into unigenomic prespore compartments, through developmentally regulated cell division and chromosome segregation events. An outer fibrous sheath, containing the lanthionine peptide SapB, the chaplins and the rodlins, confers surface hydrophobicity to aerial hyphae and spores. A dedicated ‘ Streptomyces spore wall synthesis complex’ directs the development of a thick spore wall that contributes to mature spore quiescence and resilience. Spores have reduced metabolic activity, accumulate trehalose and have condensed nucleoids. Germination involves rehydration, symmetrical swelling and establishment of polar growth, leading to germ tube emergence and vegetative hyphal outgrowth.

show abstract

Multi‐layered inhibition of Streptomyces development: BldO is a dedicated repressor of whiB

Cited by 17 publications

References 26 publications

The actinobacterial WhiB‐like (Wbl) family of transcription factors

The actinobacterial WhiB‐like (Wbl) family of transcription factors

The Streptomyces O‐B one connection: a force within layered repression of a key developmental decision

Streptomycete Spores

Contact Info

Product

Resources

About