Multi-generational effects of propranolol on Daphnia magna at different environmental concentrations

Jeong, Tae-Yong; Kim, Ho; Kim, Sang Don

doi:10.1016/j.envpol.2015.07.003

Cited by 33 publications

(9 citation statements)

References 78 publications

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“…This exposure setup can underestimate the adverse effects of chemicals as it excludes exposures during critical stages of development. As a result there is a recent shift in the academic community towards conducting multi-generational exposures for Daphnia , covering exposures during critical stages of development (Giraudo et al 2017 ; Jeong et al 2015 ; Silva et al 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Use of 5-azacytidine in a proof-of-concept study to evaluate the impact of pre-natal and post-natal exposures, as well as within generation persistent DNA methylation changes in Daphnia

et al. 2018

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Short-term exposures at critical stages of development can lead to delayed adverse effects long after the initial stressor has been removed, a concept referred to as developmental origin of adult disease. This indicates that organisms’ phenotypes may epigenetically reflect their past exposure history as well as reflecting chemicals currently present in their environment. This concept has significant implications for environmental monitoring. However, there is as yet little or no implementation of epigenetics in environmental risk assessment. In a proof-of-principle study we exposed Daphnia magna to 5-azacytidine, a known DNA de-methylating agent. Exposures covered combinations of prenatal and postnatal exposures as well as different exposure durations and recovery stages. Growth, the transcription of genes and levels of metabolites involved in regulating DNA methylation, and methylation levels of several genes were measured. Our data shows that prenatal exposures caused significant changes in the methylome of target genes, indicating that prenatal stages of Daphnia are also susceptible to same level of change as post-natal stages of Daphnia. While the combination of pre- and postnatal exposures caused the most extreme reduction in DNA methylation compared to the control group. Furthermore, some of the changes in the methylation patterns were persistent even after the initial stressor was removed. Our results suggest that epigenetic biomarkers have the potential to be used as indicators of past chemical exposure history of organisms and provide strong support for implementing changes to the current regimes for chemical risk assessment to mimic realistic environmental scenarios.

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Section: Discussionmentioning

confidence: 99%

Use of 5-azacytidine in a proof-of-concept study to evaluate the impact of pre-natal and post-natal exposures, as well as within generation persistent DNA methylation changes in Daphnia

et al. 2018

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“…In fact, it is known that heart rate was affected at much lower concentrations than the results of traditional toxicity tests, including environmental concentration. 59 …”

Section: Cardiotoxicity On D Magnamentioning

confidence: 99%

<em>Daphnia magna</em> and<em> Xenopus laevis</em> as in vivo models to probe toxicity and uptake of quantum dots functionalized with gH625

Galdiero¹,

Falanga²,

Siciliano³

et al. 2017

IJN

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The use of quantum dots (QDs) for nanomedicine is hampered by their potential toxicologic effects and difficulties with delivery into the cell interior. We accomplished an in vivo study exploiting Daphnia magna and Xenopus laevis to evaluate both toxicity and uptake of QDs coated with the membranotropic peptide gH625 derived from the glycoprotein H of herpes simplex virus and widely used for drug delivery studies. We evaluated and compared the effects of QDs and gH625-QDs on the survival, uptake, induction of several responsive pathways and genotoxicity in D. magna , and we found that QDs coating plays a key role. Moreover, studies on X. laevis embryos allowed to better understand their cell/tissue localization and delivery efficacy. X. laevis embryos raised in Frog Embryo Teratogenesis Assay- Xenopus containing QDs or gH625-QDs showed that both nanoparticles localized in the gills, lung and intestine, but they showed different distributions, indicating that the uptake of gH625-QDs was enhanced; the functionalized QDs had a significantly lower toxic effect on embryos’ survival and phenotypes. We observed that D. magna and X. laevis are useful in vivo models for toxicity and drug delivery studies.

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“…Although several methods have been established for heart rate analysis in aquatic animals, they are suffering from the drawbacks of limited usage, expensive instrument setting, or complicated operational procedures [10,12,23] (summarized in Table 2). The most updated review on analyzing the pros and cons of current cardiac rhythm detection methods in zebrafish can be found in our recently review article [24].…”

Section: Discussionmentioning

confidence: 99%

“…The low pixel intensity shows the presence of blood cells for heart relaxation, and high intensity shows the lack of blood cells for heart contraction. Jeong et al also use a similar method to quantify the heart rate of D. magna by using ToupView 3.7 software [11,12]. Zhu et al used a commercial Zebralab Blood Flow System (Viewpoint, France) [13].…”

Section: Introductionmentioning

confidence: 99%

One-Stop Method to Measure Multiple Cardiac Performance Endpoints in Daphnia and Zebrafish by Kymograph

Kurnia¹,

Saputra²,

Roldan³

et al. 2020

Preprint

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Cardiovascular disease (CVD) is the number one cause of death worldwide. This condition resulted in huge research on CVD increasing the need for animal models suitable for the in vivo research. Daphnia and Zebrafish are good animal models for cardiovascular research due to their relative body transparency and easy culture property. Several methods have been developed to conduct cardiac performance measurement in Daphnia and Zebrafish. However, most of the methods only able to obtain heartbeat rate. The other important cardiac endpoints like stroke volume, ejection fraction, fraction shortening, cardiac output and heartbeat regularity must use other programs for measurement. To overcome this limitation, in this study, we successfully developed a one-stop ImageJ-based method using kymograph macros language that able to obtain multiple cardiac performance endpoints simultaneously for the first time. To validate its utility, we incubate Daphnia magna at different ambient temperatures and exposed zebrafish with astemizole to detect the corresponding cardiac performance alterations. In summary, the kymograph method reported in this study provides a new, easy to use, and inexpensive one-stop method on obtaining multiple cardiac performance endpoints with high accuracy and convenience.

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Multi-generational effects of propranolol on Daphnia magna at different environmental concentrations

Cited by 33 publications

References 78 publications

Use of 5-azacytidine in a proof-of-concept study to evaluate the impact of pre-natal and post-natal exposures, as well as within generation persistent DNA methylation changes in Daphnia

Use of 5-azacytidine in a proof-of-concept study to evaluate the impact of pre-natal and post-natal exposures, as well as within generation persistent DNA methylation changes in Daphnia

<em>Daphnia magna</em> and<em> Xenopus laevis</em> as in vivo models to probe toxicity and uptake of quantum dots functionalized with gH625

One-Stop Method to Measure Multiple Cardiac Performance Endpoints in Daphnia and Zebrafish by Kymograph

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