Multi-Extraction and Quality of Protein and Carrageenan from Commercial Spinosum (Eucheuma denticulatum)

Abstract

Seaweeds contain many valuable compounds that can be used in the food industry. Carrageenan is a polysaccharide which has been extracted from seaweed for centuries and is used as a texturizer in food and non-food products. However, seaweeds contain compounds other than carrageenan, such as proteins, which could also be extracted. This extraction should be done without compromising the industrial scale carrageenan extraction yield and quality. This study aimed at up-stream protein extraction from red seaweed Eucheuma denticulatum by using of an optimized enzyme-assisted extraction, including of an aqueous/enzymatic treatment followed by alkaline extraction, and then the commercial carrageenan extraction. The protein extraction efficiency of four enzymes was evaluated including Celluclast® 1.5L, Shearzyme® 500 L, Alcalase® 2.4 L FG and Viscozyme® L at a concentration of 0.0, 0.1, 0.2 and 0.4% (w/w). To avoid detrimental effects on carrageenan, all the experiments were performed at pH 7 at room temperature. The results showed that 0.2% w/w Alcalase® or Viscozyme® added individually achieved the highest protein extraction efficiencies (59 and 48%, respectively) at pH 7 and room temperature (p < 0.05). Determination of the most common carrageenan quality parameters indicated that using any of these enzymes had no negative effect on the carrageenan yield and quality.

Proteomic characterization of pilot scale hot-water extracts from the industrial carrageenan red seaweedEucheuma denticulatum

“…Enzyme assisted extraction (EAE) is an emerging technology for seaweed protein extraction, showing great potential (Hardouin et al, 2016; Naseri, Marinho, Holdt, Bartela, & Jacobsen, 2020; Terme et al, 2020; Vásquez, Martínez, & Bernal, 2019). In a recent study, enzyme assisted extraction of E. denticulatum increased the protein yield up to 60% using Alcalase ® or Viscozyme ® (0.2% w/w) at pH 7 and room temperature (Naseri, Jacobsen, et al, 2020). The increased protein extraction efficiency was furthermore obtained without compromising the downstream carrageenan production.…”

“…If further extraction of other compounds such as proteins could be made prior to or as part of the industrial hot water extraction without compromising the existing carrageenan extraction, this could be of interest, since the amount of biomass available is large. Proteins from E. denticulatum were shown to constitute only 3.8% of dry biomass, but were of high quality with respect to their amino acid profile (Naseri, Jacobsen, et al, 2020). Moreover, the obtained proteins are comparable to beef in regard to the branched chained amino acids (i.e.…”

Difference Long Irradiation on The Growth Rate of Kappaphycus Alvarezii

“…Penggunaan rumput laut jenis ini semakin meningkat tidak untuk industri makanan saja tetapi juga sebagai bahan baku produk kecantikan, obat-obatan, dan bahan baku untuk kegiatan industri lainnya. Rumput laut dari jenis Kappaphycus alvarezii dapat menghasilkan hidrokolid karagenan yang penting secara komersial (Isa et al, 2016;Naseri et al, 2020). Karagenan mempunyai kemampuan untuk membentuk gel.…”

Mechanical Disintegration and Particle Size Sieving of Chondrus crispus (Irish Moss) Gametophytes and Their Effect on Carrageenan and Phycoerythrin Extraction

“…A biorefinery approach, through implementing additional steps before or after the carrageenan extraction, seems a promising way forward [ 17 ]. The use of enzymes to separate seaweed proteins and carrageenan has also been proposed [ 18 ]. An equally important approach is to understand the role of each extraction parameter and how it affects the carrageenan extraction.…”