Multi Epitope Vaccine Prediction Against Aichi Virus using Immunoinformatic Approach

Abstract

Aichi virus, AiV is single stranded negative sense RNA genome belonging to the genus Kobuviru, a family of Picornaviridae that causes severe gastroenteritis. There is no treatment or vaccine for it, thus the aim of this study is to design a peptide vaccine using immunoinformatic approaches to analyze the viral Protein VP1 of AiV-1 strain, to determine the conserved region which is further studied to predict all possible epitopes that can be used as a peptide vaccine. A total of 38 Aichi virus VP1 retrieved from NCBI database were aligned to determine the conservancy and to predict the epitopes using IEDB analysis resource. Three epitopes predicted as a peptide vaccine for B cell was (PLPPDT, PPLPTP, and LPPLPTP). For T cell, two epitopes showed high affinity to MHC class I (FSIPYTSPL and TMVSFSIPY) and high coverage against the whole world population. Also, in MHC class II, three epitopes that interact with most frequent MHC class II alleles (FTYIAADLR and YMAEVPVSA) with high coverage in the whole world population. For both MHCI and MHCII the T-cell peptide with the strongest affinity to the worldwide population was FSIPYTSPL.Peptide vaccine against AiV is powerfully displace the normal produced vaccines based on the experimental biochemistry tools, as it designed to handle with a wide range of mutated strains, which will effectively minimize the frequent outbreaks and their massive economical wastage consequences.

Identification of Promising CD8 and CD4 T-cell Epitopes for Peptide Vaccine Formulation Against SARS-CoV-2

“…LPPLPTP and PLPPDT and one T-cell epitope FSIPYTSPL (binds to both MHC-and MHC-) were found to be e cient targets for boosting immunity against this virus [19].…”

Identification of promising CD8 and CD4 T cell epitopes for peptide vaccine formulation against SARS-CoV-2

“…Aichi virus 1 (AiV-1) of Picornaviridae family causes gastroenteritis in humans and it is hazardous for children in the developing countries. From a study on this virus, three B cell epitopes, i.e., PPLPTP, LPPLPTP and PLPPDT and one T cell epitope FSIPYTSPL (binds to both MHC-I and MHC-II) were found to be efficient targets for boosting immunity against this virus (Hassan et al 2019 ).…”

Cefaleia Pós-Punção Dural Em Raquianestesia: Uma Revisão Bibliográfica Sobre Incidência, Fatores De Risco E Estratégias De Manejo

“…A CPPD não apenas afeta a saúde dos pacientes, mas também tem um impacto econômico significativo. A prolongação do tempo de internação e a necessidade de intervenções adicionais, como o blood patch epidural, aumentam os custos hospitalares (Ali;Ahmed et al, 2019). Além disso, a CPPD pode resultar em perda de produtividade devido à incapacidade temporária dos pacientes de retornar ao trabalho (Oberhofer et al, 2013).…”