Multi-elemental imaging of paraffin-embedded human samples by laser-induced breakdown spectroscopy

Moncayo, S.; Trichard, Florian; Busser, Benoît; Sabatier-Vincent, M.; Pelascini, Frédéric; Pinel, N.; Templier, I.; Charles, J.; Sancey, Lucie; Motto-Ros, Vincent

doi:10.1016/j.sab.2017.04.013

Cited by 56 publications

(40 citation statements)

References 31 publications

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“…As natural pigmentation is a frequent problem in immunohistochemistry, new non-optical detection methods are desired. Mass-spectrometry methods, either label-free methods such as MALDI MSI or employing metal labels such as laser-induced breakdown spectroscopy (LIBS) or ICP-MS, have the potential to overcome this difficulty [ 30 – 32 ]. Several tissue markers, including S100, MART-1, gp100/HMB45 and/or MT, are used to help distinguish melanoma from other types of cancer [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles

et al. 2017

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BackgroundTreatment of advanced cutaneous melanoma remains challenging, and new data on melanoma biology are required. The most widely accepted criteria for the prognostic evaluation of melanoma are histopathological and clinical parameters, and the identification of additional tumor markers is thus of paramount importance. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI), an important tool in cancer research, is useful for unraveling the molecular profile of melanoma.Methodology/Principal findingsIn this report, we used the melanoma-bearing Libechov minipig (MeLiM), a unique animal model that allows observation of the complete spontaneous regression of invasive cutaneous melanoma, to investigate i) the differences between melanoma and healthy skin protein profiles and ii) the proteins potentially involved in spontaneous regression. The MeLiM tissues were cryosected, histologically characterized, analyzed by MALDI MSI, and immunohistologically stained. Multivariate statistical analyses of the MALDI MSI data revealed ten relevant m/z ions, of which the expression levels varied significantly among the studied MeLiM tissues. These ion peaks were used to create mass ion images/maps and visualize the differences between tumor and healthy skin specimens, as well as among histologically characterized tissue regions.Conclusions/SignificanceProtein profiles comprising ten statistically significant mass ion peaks useful for differentiating cutaneous melanoma and healthy skin tissues were determined. Peaks at m/z 3044, 6011, 6140 and 10180 were overexpressed in melanoma compared with healthy skin tissue. More specifically, m/z 6140 was expressed at significantly (p < 0.05) higher levels in normally growing melanoma regions than in regions with early and late spontaneous regression. This study demonstrates the clinical utility of MALDI MSI for the analysis of tissue cryosections at a molecular level.

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Section: Discussionmentioning

confidence: 99%

MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles

et al. 2017

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“…For example, Sancey et al 17 reported that the elements in mouse kidney embedded with Gd-based nanoparticles could be imaged with 10-μm resolution using a nanosecond (ns) 266 nm Nd:YAG laser-based LIBS. Also, Moncayo et al 18 took LIBS image of different types of paraffin embedded human tissue. Bonta et al 19 used LIBS for elemental mapping of human tumor in conjunction with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), where ns-LIBS was used for the mapping of major and minor elements while LA-ICP-MS was for trace elements.…”

Section: Introductionmentioning

confidence: 99%

Mapping of cutaneous melanoma by femtosecond laser-induced breakdown spectroscopy

et al. 2018

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Surgical excision (Mohs micrographic surgery) is the standard procedure to treat a melanoma, in which an in situ histologic examination of sectioned skin is carried out repeatedly until no cancer cells are detected. The possibility to identify melanoma from the surrounding skin by femtosecond laser-induced breakdown spectroscopy (fs-LIBS) is investigated. For experiments, melanoma induced on a hairless mouse by injection of B16/F10 murine melanoma cell was sampled in the form of frozen tissue sections as in Mohs surgery and analyzed by fs-LIBS (λ ¼ 1030 nm, τ ¼ 550 fs). For analysis, the magnesium signal normalized by carbon intensity was utilized to construct an intensity map around the cancer, including both melanoma and surrounding dermis. The intensity map showed a close match to the optically observed morphological and histological features near the cancer region. The results showed that when incorporated into the existing micrographic surgery procedure, fs-LIBS could be a useful tool for histopathologic interpretation of skin cancer possibly with significant reduction of histologic examination time.

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“…115 As imagens hiperespectrais podem ser empregadas para avaliar a localização e distribuição espacial de nutrientes em material vegetal, 116 macronutrientes em alimentos, 117 elementos perigosos, preciosos e estratégicos em lixo eletrônico, 118 análises de fósseis e coprólitos, 45 materiais arqueológicos 119 e a discriminação entre pele humana saudável e com câncer. 108 Outra interessante abordagem qualitativa dos espectros LIBS é na identificação e proposição de modelos de classificação, empregando diferentes ferramentas quimiométricas. Senesi et al 120 identificaram e classificaram amostras de meteoritos utilizando os espectros obtidos de um equipamento LIBS portátil, usando algoritmo de inferência baseado em lógica fuzzy.…”

Section: Abordagens Para Análise Qualitativa E Quantitativaunclassified

Laser Induced-Breakdown Spectroscopy (Libs): Histórico, Fundamentos, Aplicações E Potencialidades

Costa¹,

Augusto²,

Castro³

et al. 2019

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This review attempts to present a broad view of the technique laser-induced breakdown spectroscopy (LIBS), since its origin, fundamentals, sample preparation strategies, use of chemometric tools for data processing, the advantages and limitations with regard to qualitative and quantitative analysis, aplications and future trends as an analytical technique that is in consolidation in analytical chemistry. LIBS is a rapid and versatile, which is able of providing qualitative and quantitative analytical information for practically any sample, in a virtually non-destructive way, without any substantial sample preparation. The instrumentation is simple, robust, compact, and enables remote analysis. The advancement of the LIBS applications in the areas of agriculture, environmental, food, biological/pharmaceutical, forensic, metallurgy and electronic waste were discussed in detail in this review. We consider that this review will be an important tool concerning dissemination of the LIBS, mainly for audience of undergraduate courses, and disciplines related to instrumental analysis.

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Multi-elemental imaging of paraffin-embedded human samples by laser-induced breakdown spectroscopy

Cited by 56 publications

References 31 publications

MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles

MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles

Mapping of cutaneous melanoma by femtosecond laser-induced breakdown spectroscopy

Laser Induced-Breakdown Spectroscopy (Libs): Histórico, Fundamentos, Aplicações E Potencialidades

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