Multi-drug-resistant enterotoxigenic and enterohemorrhagic<i>Escherichia coli</i>isolated from children with diarrhea

Zeighami, Habib; Haghi, Fakhri; Hajiahmadi, Fahimeh; Kashefiyeh, Mehdi; Memariani, Mojtaba

doi:10.1179/1973947813y.0000000161

Cited by 19 publications

(17 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increasing prevalence of multi-drug resistance in DEC strains is alarming. In our current study, the resistance rate to third generation cephalosporins (ceftriaxone, ceftazidime, cefotaxime and ceftizoxime) was higher than those reported in other studies [6,[35][36][37]. Ceftriaxone and ceftazidime are widely-used antibiotics for the treatment of infectious diarrhea in children in Iran which may be a possible explanation for the high rate of DEC resistance to third generation cephalosporins.…”

Section: Discussioncontrasting

confidence: 59%

Characterization of diarrheagenic Escherichia coli strains associated with diarrhea in children, Khouzestan, Iran

Broujerdi

Ardakani

Rezatofighi

2018

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Diarrheagenic Escherichia coli (DEC) is a major etiologic agent among the pathogens that cause diarrhea in children. Methodology: To investigate the presence and pathotypes of DEC in children under five years of age, living in the province of Khouzestan, Iran. 208 diarrhea stool samples were screened by multiplex-PCR. The isolated DEC isolates were investigated for resistance to various antimicrobials including the production of extended-spectrum beta-lactamases (ESBLs) and phylogenetic groups were determined. Results: DEC isolates were identified in 54 (26%) diarrhea samples, and 4 (7%) cases contained two DEC pathotypes. DEC isolated included 35 (16.8%) enteroaggregative E. coli (EAEC), ten (4.8%) enteropathogenic E. coli (EPEC), six (2.9%) enteroinvasive E. coli (EIEC), six (2.9%) enterotoxigenic E. coli (ETEC) and one (0.48%) LEE-positive EAEC. Shiga-toxin producing E. coli (STEC) was not identified in any diarrheal samples. The most prevalent resistance was observed with ceftazidime (88%), followed by ceftizoxime (83%) and ceftriaxone (71%). The majority of isolates (> 75%) were sensitive to Imipenem, ciprofloxacin, and amikacin. More than 65% of the pathogenic isolates showed a multidrug-resistant phenotype. ESBL-producing strains was observed in 79.3% of all DEC isolates. Phylogenetic group B2 was the most predominant group with a frequency of 44.8%. A significant association was observed between the B2 phylogenetic group and the DEC isolates (P < 0.05). Conclusions: Overall, our findings highlight the importance of the role of DEC isolates in the etiology of diarrhea in children in Iran. The progressive increase in antimicrobial resistance among DEC isolates makes it imperative to implement policies to control the spread of resistant bacteria.

show abstract

Section: Discussioncontrasting

confidence: 59%

Characterization of diarrheagenic Escherichia coli strains associated with diarrhea in children, Khouzestan, Iran

Broujerdi

Ardakani

Rezatofighi

2018

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Table 3. Phenotypic antibiotic resistance rates in E. coli pathotypes MDR DEC has quickly spread worldwide and represents a serious menace to the proper management of diarrhea and dysentery in developing countries (19). The frequencies of MDR DEC in the present study and in others are summarized in Table 5.…”

Section: Phenotypic and Genotypic Investigationmentioning

confidence: 69%

Multi-drug-resistant diarrheagenic Escherichia coli pathotypes in pediatric patients with gastroenteritis from central Iran

Abbasi

Mondanizadeh

Belkum

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Diarrheagenic Escherichia coli (DEC) is a significant cause of gastroenteritis and a major public health problem. This study investigates the prevalence and the antibiotic resistance patterns of DEC that were isolated from infectious diarrhea samples of pediatric patients from central Iran.Methods Pediatric diarrhea samples were collected from 230 pediatric patients visiting the hospital. E. coli pathotypes were diagnosed by using conventional culture methods and PCR. Antibiotic resistance profiles, the frequency of multi-drug resistance (MDR), and the phenotypic and genotypic characteristics of extended spectrum-β-lactamase (ESBL), AmpC and integron-associated genes were analyzed.Results Of the 230 samples of infectious diarrhea, 91 (39.5%) produced E. coli isolates. Of these, 32 cases (35.1%) were identified as DEC by culture and PCR. The frequency of the E. coli pathotypes obtained was as follows: EAEC 11/32 (34.3%), EPEC 9/32 (28.1%), ETEC 6/32 (18.7%), EIEC 3/32 (9.3%), and EHEC 3/32 (9.3%). The antibiotic resistance rates were greater for nalidixic acid (30/32; 93.7%), ampicillin (29/32; 90.6%), and tetracycline (25/32; 78.1%) than for any of the other tested antibiotics. High levels of MDR (25/32; 78.1%) and the presence of ESBL (18/32; 56.2%) and AmpC (9/32; 28.1%) were observed in the DEC isolates. The isolates showed a higher frequency of the ESBL genes [ bla TEM (18/18; 100%), bla CTX-M15 (17/18; 94.4%)], and AmpC [ bla CIT (4/9; 44.4%) and bla DHA (4/9; 44.4%)] than of the other ESBL and AmpC genes.Conclusion DEC appeared to be the second-most abundant agent of diarrhea in pediatric patients after Campylobacter jejuni , with frequent MDR and ESBL presence.

show abstract

“…The frequency up to date reported for MDR ETEC isolates is 46.4% (Zeighami et al, 2014 ). In this study, 65% of ETEC isolates positive for lngA , were multidrug-resistant to different antibiotic categories.…”

Section: Discussionmentioning

confidence: 99%

CS21 positive multidrug-resistant ETEC clinical isolates from children with diarrhea are associated with self-aggregation, and adherence

et al. 2014

View full text Add to dashboard Cite

Background: Enterotoxigenic Escherichia coli (ETEC) colonize the human intestinal mucosa using pili and non-pili colonization factors (CFs). CS21 (also designated Longus) is one of the most prevalent CFs encoded by a 14 kb lng DNA cluster located in a virulence plasmid of ETEC; yet limited information is available on the prevalence of CS21 positive ETEC isolates in different countries. The aim of this study was to evaluate the prevalence of CS21 among ETEC clinical isolates from Mexican and Bangladeshi children under 5 years old with diarrhea and to determine the phenotypic and genotypic features of these isolates.Methods: ETEC clinical isolates positive to lngA gene were characterized by genotype, multidrug-resistance, self-aggregation, biofilm formation, and adherence to HT-29 cell line.Results: A collection of 303 E. coli clinical isolates were analyzed, the 81.51% (247/303) were identified as ETEC, 30.76% (76/247) were st+/lt+, and 25.10% (62/247) were positive for the lngA gene. Among the lngA+ ETECs identified, 50% of isolates (31/62) were positive for LngA protein. The most frequent serotype was O128ac:H12 found in 19.35% (12/62) of lngA+ ETEC studied. Multidrug-resistance (MDR) lngA+ ETEC isolates was identified in 65% (39/60), self-aggregation in 48.38% (30/62), and biofilm formation in 83.87% (52/62). ETEC lngA+ isolates were able to adhere to HT-29 cells at different levels. Two lngA isogenic mutants were constructed in the ETEC E9034A and ETEC73332 clinical isolate, showing a 77% and 98% reduction in adherence, respectively with respect to the wild type.Conclusion: ETEC isolates that have the lngA gene showed features associated with self-aggregation, and adherence to HT-29 cells, important characteristics in the human gut colonization process and pathogenesis.

show abstract

Multi-drug-resistant enterotoxigenic and enterohemorrhagicEscherichia coliisolated from children with diarrhea

Cited by 19 publications

References 20 publications

Characterization of diarrheagenic Escherichia coli strains associated with diarrhea in children, Khouzestan, Iran

Characterization of diarrheagenic Escherichia coli strains associated with diarrhea in children, Khouzestan, Iran

Multi-drug-resistant diarrheagenic Escherichia coli pathotypes in pediatric patients with gastroenteritis from central Iran

CS21 positive multidrug-resistant ETEC clinical isolates from children with diarrhea are associated with self-aggregation, and adherence

Contact Info

Product

Resources

About