Multi-dimensional chromatography using different developing methods

The Chemistry of Tetracyanoethylene

“…The property of TCNE to form intensely colored complexes at room temperature with many aromatic compounds makes it useful as a color-forming reagent in the paper chromatography of these compounds (62,63,121,152). It has also been used in the estimation of aliphatic, alicyclic, and aromatic 1,3-dienes (117).…”

Chromatography

“…For the application of a sample in the form of a line, especially in preparative work, it may be run up to the origin in a solvent in which it migrates with the front (512,2105) or applied between two parallel cuts, which are later filled in (328). The effluent from gas chromatographs has been collected directly on thin-layer plates for further analysis (886,926,1405). TLC was also combined with the ring oven method (1478, 1781), and organic compounds have been subjected to various reactions prior to development (1280).…”

“…Detection reagents include: pyrosulfuric acid ( 2016), chlorosulfonic acid-acetic acid (1:2) (703), ozone, followed by indigo (1491), ceric nitrate (607, 1788), cobalt carbonyl (1804), ultraviolet light alone (1117,1288) or after treatment with dichlorofluorescein ( 1246), acids or piperonal (1764), or various quenchers (1763). Tetracy anoethylene may be used as a spray (1541) or incorporated into the adsorbent (886).…”