Multi-dimensional bioinspired tactics using an engineered mussel protein glue-based nanofiber conduit for accelerated functional nerve regeneration

Cheong, Hogyun; Kim, Jimin; Kim, Bum Jin; Kim, Eun Jin; Park, Hae-Yeon; Choi, Bong‐Hyuk; Joo, Kye Il; Cho, Mi‐La; Rhie, Jong Won; Lee, Jong In; Joon, Hyung

doi:10.1016/j.actbio.2019.04.018

Cited by 35 publications

(30 citation statements)

References 69 publications

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“…Similar to in vitro findings [120,121,[146][147][148][149][150], functionalizing electrospun fibers with biochemical cues can significantly enhance their ability to improve peripheral nerve regeneration in vivo [175,176]. Cheong et al fabricated aligned PLGA electrospun fibers loaded with mussel adhesive proteins fused to bifunctional peptides derived from two different regions of the laminin (IKVAV or YIGSR) protein or the fibronectin protein (GRGDS).…”

Section: Schwann Cell Response To Fiber Functionalization and Therapementioning

confidence: 86%

“…The PLGA fibers functionalized with IKVAV performed best in vitro, supporting proliferation, elongation, and differentiation of PC12 neuronal cells and S16 Schwann cells, and this scaffold was selected to implant into a 15-mm rat sciatic nerve injury model. In vivo, the IKVAV functionalized fiber graft supported the repopulation of the graft by neurons and Schwann cells and significantly enhanced remyelination, compared to nonfunctionalized control grafts and the autograft group [175]. In another example, Rao et al fabricated an aligned electrospun chitosan nanofiber hydrogel functionalized with the growth factormimicking peptides, RGI, derived from BDNF, and KLT, derived from vascular endothelial growth factor.…”

Section: Schwann Cell Response To Fiber Functionalization and Therapementioning

confidence: 99%

“…These findings, summarized in Table 3, will provide a foundation for future construction of synthetic nerve grafts that aim to enhance regenerative outcomes to levels beyond that of autografts. Improve Schwann cell infiltration of nerve grafts [175] and remyelination of regenerated axons [175,176].…”

Section: Summary Of Schwann Cell Response To Electrospun Fibers In Vivomentioning

confidence: 99%

“…Increase astrocyte infiltration into the scaffold [175,221,244]. Reduce glial scarring [240,[242][243][244].…”

Section: Astrocytesmentioning

confidence: 99%

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Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

et al. 2020

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Electrospinning is a fabrication technique used to produce nano- or micro- diameter fibers to generate biocompatible, biodegradable scaffolds for tissue engineering applications. Electrospun fiber scaffolds are advantageous for neural regeneration because they mimic the structure of the nervous system extracellular matrix and provide contact guidance for regenerating axons. Glia are non-neuronal regulatory cells that maintain homeostasis in the healthy nervous system and regulate regeneration in the injured nervous system. Electrospun fiber scaffolds offer a wide range of characteristics, such as fiber alignment, diameter, surface nanotopography, and surface chemistry that can be engineered to achieve a desired glial cell response to injury. Further, electrospun fibers can be loaded with drugs, nucleic acids, or proteins to provide the local, sustained release of such therapeutics to alter glial cell phenotype to better support regeneration. This review provides the first comprehensive overview of how electrospun fiber alignment, diameter, surface nanotopography, surface functionalization, and therapeutic delivery affect Schwann cells in the peripheral nervous system and astrocytes, oligodendrocytes, and microglia in the central nervous system both in vitro and in vivo. The information presented can be used to design and optimize electrospun fiber scaffolds to target glial cell response to mitigate nervous system injury and improve regeneration.

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Section: Schwann Cell Response To Fiber Functionalization and Therapementioning

confidence: 86%

Section: Schwann Cell Response To Fiber Functionalization and Therapementioning

confidence: 99%

Section: Summary Of Schwann Cell Response To Electrospun Fibers In Vivomentioning

confidence: 99%

“…Increase astrocyte infiltration into the scaffold [175,221,244]. Reduce glial scarring [240,[242][243][244].…”

Section: Astrocytesmentioning

confidence: 99%

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Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

et al. 2020

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“…Nanoscaffolds containing IKVAV significantly improved functional recovery after traumatic brain injury (TBI) [26], and nanofibers containing IKVAV blended with PLGA effectively promoted nerve and SC attachment and migration. Moreover, a PLGA/IKVAV-modified conduit was shown to promote axonal regeneration and functional recovery following creation of a 15 cm sciatic nerve gap [27]. Thus, IKVAV shows promise for functionalizing various biomaterial scaffolds to promote nerve regeneration at severe injury sites.…”

Section: Introductionmentioning

confidence: 99%

Construction of Dual-Biofunctionalized Chitosan/Collagen Scaffolds for Simultaneous Neovascularization and Nerve Regeneration

Han

et al. 2020

Research

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Biofunctionalization of artificial nerve implants by incorporation of specific bioactive factors has greatly enhanced the success of grafting procedures for peripheral nerve regeneration. However, most studies on novel biofunctionalized implants have emphasized the promotion of neuronal and axonal repair over vascularization, a process critical for long-term functional restoration. We constructed a dual-biofunctionalized chitosan/collagen composite scaffold with Ile-Lys-Val-Ala-Val (IKVAV) and vascular endothelial growth factor (VEGF) by combining solution blending, in situ lyophilization, and surface biomodification. Immobilization of VEGF and IKVAV on the scaffolds was confirmed both qualitatively by staining and quantitatively by ELISA. Various single- and dual-biofunctionalized scaffolds were compared for the promotion of endothelial cell (EC) and Schwann cell (SC) proliferation as well as the induction of angiogenic and neuroregeneration-associated genes by these cells in culture. The efficacy of these scaffolds for vascularization was evaluated by implantation in chicken embryos, while functional repair capacity in vivo was assessed in rats subjected to a 10 mm sciatic nerve injury. Dual-biofunctionalized scaffolds supported robust EC and SC proliferation and upregulated the expression levels of multiple genes and proteins related to neuroregeneration and vascularization. Dual-biofunctionalized scaffolds demonstrated superior vascularization induction in embryos and greater promotion of vascularization, myelination, and functional recovery in rats. These findings support the clinical potential of VEGF/IKVAV dual-biofunctionalized chitosan/collagen composite scaffolds for facilitating peripheral nerve regeneration, making it an attractive candidate for repairing critical nerve defect. The study may provide a critical experimental and theoretical basis for the development and design of new artificial nerve implants with excellent biological performance.

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Historical Developments of Various Adhesives for Biomedical Applications

Kommineni,

Saka,

Sainaga Jyothi

et al. 2023

Adhesives in Biomedical Applications

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Multi-dimensional bioinspired tactics using an engineered mussel protein glue-based nanofiber conduit for accelerated functional nerve regeneration

Cited by 35 publications

References 69 publications

Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

Construction of Dual-Biofunctionalized Chitosan/Collagen Scaffolds for Simultaneous Neovascularization and Nerve Regeneration

Historical Developments of Various Adhesives for Biomedical Applications

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