Multi-coloured fluorescent sensing toolbox for selective detection of nitroxyl in vitro and ex vivo

Abstract

Methods for the endogenous detection of nitroxyl (azanone; HNO), the reduced and protonated derivative of nitric oxide (NO), are required to define its cardiovascular function and its key role in chronic pain.

A fluorescence “turn-on” probe for the respective ratiometric detection of hypochlorite and cysteine

“…These molecules undergo inter-reaction and inter-conversion to facilitate the dynamic balance of the intracellular redox state, among which three types of representative molecules should be mentioned, including reactive oxygen (H 2 O 2 , HClO, O 2 ˙−, 1 O 2 , ˙OH), reactive nitrogen (ONOO − , NO 2 − , HNO − ), and reactive sulfur (GSH, Cys, Hcy, H 2 S, RS˙) species. 1,2 As a normal byproduct of cellular metabolism, hypochlorous acid (HClO) is mainly involved in the regulation of cellular signaling transduction and immune response. It can be produced in immunological cells from hydrogen peroxide and chloride ions mediated by myeloperoxidase.…”