Multi-color quantum dot-based fluorescence immunoassay array for simultaneous visual detection of multiple antibiotic residues in milk

Song, Erqun; Yu, Mengqun; Wang, Yunyun; Hu, Weihua; Cheng, Dan; Swihart, Mark T.; Song, Yang

doi:10.1016/j.bios.2015.05.018

Cited by 190 publications

(63 citation statements)

References 38 publications

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“…So these complicated operation steps may limit its application. Compared to ELISA, a new approach called fluorescence-linked immunosorbent assay (FLISA) has been developed recently [8–10]. The emission of fluorescent signal as the analytical signal can be immediately recorded by the microplate reader, and it reduces the analysis time because of no chromogenic reaction steps of ELISA [11].…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Feng³

et al. 2017

J Nanobiotechnol

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BackgroundThe conventional and widely used enzyme-linked immunosorbent assays (ELISA), due to specificity and high-sensitivity, were suitable in vitro diagnosis. But enzymes are vulnerable to the external conditions, and the complex operation steps limit its application. Semiconductor quantum dots have been successfully used in biological and medical research due to the high photoluminescence and high resistance to photobleaching. In this study, we have developed a novel quantum dot-labeled immunosorbent assay for rapid disease detection of C-reactive protein (CRP).ResultsThe assay for the detection of CRP can provide a wide analytical range of 1.56–400 ng/mL with the limit of detection (LOD) = 0.46 ng/mL and the limit of quantification = 1.53 ng/mL. The precision of the assay has been confirmed for low coefficient of variation, less than 10% (intra-assay) and less than 15% (inter-assay). The accuracy of assay meets the requirements with the recoveries of 95.4–105.7%. Furthermore, clinical samples have been collected and used for correlation analysis between this FLISA and gold standard Roche immunoturbidimetry. It shows excellent accurate concordance and the correlation coefficient value (R) is as high as 0.989 (n = 34).ConclusionsThis in vitro quantum dot-based detection method offers a lower LOD and a wide liner detection range than ELISA. The total reaction time is only 50 min, which is much shorter than the commercialization ELISA (about 120 min). All of the results show that a convenient, sensitive, and accurate fluorescence-linked immunosorbent assay method has been well established for the detection of CRP samples. Therefore, this method has immense potential for the development of rapid and cost-effective in vitro diagnostic kits.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-017-0267-4) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Feng³

et al. 2017

J Nanobiotechnol

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“…Another group reported a similar AuNP-based colorimetric method for the detection of streptomycin in milk [134]. A recently reported biosensor for the detection of streptomycin and other classes of ABs is based on a multicolor quantum dot based immunofluorescence assay [135].…”

Section: Non-chromatographic Methodsmentioning

confidence: 99%

Challenges in the determination of aminoglycoside antibiotics, a review

Farouk

Azzazy

Niessen

2015

Analytica Chimica Acta

120

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“…Amoxicillin (AMOX) is a semi‐synthetic antibiotic used to treat many bacterial infections. Amoxicillin has also been described as an antibiotic with higher absorption in humans and animals . Improper use of antibiotics leads to residues in foodstuffs.…”

Section: Introductionmentioning

confidence: 99%

Surface Plasmon Resonance Nanosensors for Detecting Amoxicillin in Milk Samples with Amoxicillin Imprinted Poly(hydroxyethyl methacrylate‐N‐methacryloyl‐(L)‐ glutamic acid)

et al. 2020

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In this study, SPR based nanosensors were prepared for the detection of amoxicillin (AMOX) in milk samples with using molecular imprinting technique. This study developed to determine the amoxicillin (AMOX) from the commercial and local milk samples. For determination of amoxicillin, polymeric film and nanoparticles consisting of poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-glutamic acid) were synthesized onto the surface of the SPR chip by ultra violet polymerization. The characterization of the polymeric film and nanoparticles were determined by zeta sizer, ellipsometry, contact angle and atomic force microscopy measurements. The range of linearity was measured as 0.1-200 ng/mL for amoxicillin (AMOX) imprinted polymeric film and nanoparticles based SPR nanosensors. The limit of detection for AMOX imprinted polymeric film and nanoparticles based SPR nanosensors were found as 0.0012 ng/mL and 0.0009 ng/mL, respectively. To show the selectivity of surface plasmon resonance nanosensors competitive adsorption of amoxicillin (AMOX), ampicillin (AMP), cephalexin (CEP) and cholesterol (KOL) were investigated. According to the results, AMOX imprinted SPR nanosensors have exhibited low-detection limit, high sensitivity and selectivity for detection of antibiotic in milk samples.

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Multi-color quantum dot-based fluorescence immunoassay array for simultaneous visual detection of multiple antibiotic residues in milk

Cited by 190 publications

References 38 publications

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Challenges in the determination of aminoglycoside antibiotics, a review

Surface Plasmon Resonance Nanosensors for Detecting Amoxicillin in Milk Samples with Amoxicillin Imprinted Poly(hydroxyethyl methacrylate‐N‐methacryloyl‐(L)‐ glutamic acid)

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