Multi-clonal expansion of unique human T-lymphotropic virus type-I-infected T cells with high growth potential in response to interleukin-2 in prodromal phase of adult T cell leukemia

Hata, Tomoko; Fujimoto, Takeshi; Tsushima, Hiroyuki; Murata, Ken T.; Tsukasaki, Kunihiro; Atogami, Sunao; Sohda, Hisashi; Honda, Sumihisa; Mine, Mariko; Yamada, Yasuaki; Ikeda, Shu‐ichi; Kamihira, Shimeru; Tomonaga, Masao

doi:10.1038/sj.leu.2401271

Cited by 12 publications

(9 citation statements)

References 33 publications

(14 reference statements)

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“…PBLs of ATL patients and normal donors were cultured in vitro with IL-2 in 10% fetal calf serum (FCS)-RPMI-1640 medium, in which T-cells are known to grow preferentially. 39,40) Growth of ATL PBLs was inhibited by either TEA or EGCg more than that of normal PBLs, suggesting that atypical T lymphocytes of ATL PBLs may be relatively sensitive to TEA and EGCg, especially the case 1 and case 3 ATL PBLs (Fig. 1, A and B).…”

Section: Discussionmentioning

confidence: 96%

Green Tea Polyphenols Induce Apoptosis in vitro in Peripheral Blood T Lymphocytes of Adult T‐Cell Leukemia Patients

Yashiki

Sonoda

et al. 2000

Japanese Journal of Cancer Research

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Green tea polyphenols (TEA) are known to exhibit antioxidative activity as well as tumor-suppressing activity. In order to examine the tumor-suppressing activity of TEA against adult T-cell leukemia (ATL), we cultivated peripheral blood T lymphocytes of ATL patients (ATL PBLs), an HTLV-I-infected T-cell line (KODV) and healthy controls (normal PBLs) for 3 days in the presence of TEA and its main constituent, epigallocatechin-3-gallate (EGCg), to measure cell proliferation and apoptosis, and to quantitate mRNAs of HTLV-I pX and β β β β-actin genes of the cultured cells. Growth of ATL

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Section: Discussionmentioning

confidence: 96%

Green Tea Polyphenols Induce Apoptosis in vitro in Peripheral Blood T Lymphocytes of Adult T‐Cell Leukemia Patients

Yashiki

Sonoda

et al. 2000

Japanese Journal of Cancer Research

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“…All the human T-cell lines used were described previously (Nagata et al, 1989;Yamada et al, 1996;Hata et al, 1999; Involvement of Fra-2 and JunD in ATL oncogenesis T Nakayama et al Yoshie et al, 2002). Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples obtained from healthy adult donors and acute ATL patients with a high leukemic cell count (>90%) by using Ficoll-Paque (Amersham Biosciences Corp, Piscataway, NJ, USA).…”

Section: Cellsmentioning

confidence: 99%

Aberrant expression of Fra-2 promotes CCR4 expression and cell proliferation in adult T-cell leukemia

et al. 2007

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“…Since ATL cells frequently invade and proliferate in BM, especially in aggressive phase, this co-culture system is considered to provide components which are necessary for growth and survival of neoplastic cells in BM microenvironment. Surprisingly, we reported previously that in a semi-solid colony assay system, IL-2-dependent colony formation occurred in almost all cases of chronic or smoldering type, but rarely in acute and lymphoma types [55]. These results observed among our limited number of clinical cases indicate that in the prodromal phase of ATL, some cytokines were needed for the growth of neoplastic cells in vitro, while in a more aggressive phase, such a cytokine-dependency declines and some aberrant signal transducers might contribute to a more autonomous proliferation and enhance survival of ATL cells through antiapoptotic process, which is considered to be closely related to stroma-dependent cell growth.…”

Section: Discussionmentioning

confidence: 90%

Adhesion-dependent growth of primary adult T cell leukemia cells with down-regulation of HTLV-I p40Tax protein: a novel in vitro model of the growth of acute ATL cells

et al. 2008

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In order to better understand the biology of adult T cell leukemia (ATL), we aimed to establish a novel method, which allows the primary growth of ATL cells using a co-culture system with murine bone marrow-derived stromal cells, MS-5. ATL cells grew in close contact with MS-5 layers and formed so-called "cobblestone areas" (CAs) without the addition of IL-2. In clinical samples, eight of ten (80.0%) cases of acute or lymphoma type ATL cells formed CAs. The frequency of CA forming cells in ATL cells ranged from 0.03 to 1.04%. The morphology, immunophenotyping, and DNA analysis indicated that cells composing CA were compatible with ATL cells, and clonally identical to primary CD4-positive ATL cells. Furthermore, in ATL cells composing CA, the expression of p40Tax was down-regulated in transcriptional and translational level, while that of HTLV-I basic leucine zipper factor (HBZ) gene was comparable to the level of primary ATL cells, resembling expression pattern of proviral genes in in vivo ATL cells. By microarray analysis, several genes which coded products involved in cell-cell interaction, and cellular survival and proliferation, were differentially expressed in ATL cells composing CA compared with primary samples. In conclusion, our co-culture system allows for the first time the growth of primary ATL cells in vitro, and might be useful as an in vitro assay for biological and clinical studies to develop molecular targeting drugs against ATL.

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Multi-clonal expansion of unique human T-lymphotropic virus type-I-infected T cells with high growth potential in response to interleukin-2 in prodromal phase of adult T cell leukemia

Abstract: We established a simple IL-2-dependent colony-forming assay for T cells infected with human T-lymphotropic virus type-I (HTLV-I). IL-

Cited by 12 publications

References 33 publications

Green Tea Polyphenols Induce Apoptosis in vitro in Peripheral Blood T Lymphocytes of Adult T‐Cell Leukemia Patients

Green Tea Polyphenols Induce Apoptosis in vitro in Peripheral Blood T Lymphocytes of Adult T‐Cell Leukemia Patients

Aberrant expression of Fra-2 promotes CCR4 expression and cell proliferation in adult T-cell leukemia

Adhesion-dependent growth of primary adult T cell leukemia cells with down-regulation of HTLV-I p40Tax protein: a novel in vitro model of the growth of acute ATL cells

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