Multi-Class Procedure for Analysis of 50 Antibacterial Compounds in Eggshells Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Gbylik-Sikorska, Małgorzata; Gajda, Anna; Nowacka-Kozak, Ewelina; Łebkowska‐Wieruszewska, Beata; Posyniak, Andrzej

doi:10.3390/molecules26051373

Cited by 5 publications

(6 citation statements)

References 31 publications

(32 reference statements)

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“…The LC-MS/MS analysis for all matrices was performed using the analytical method previously described by Gbylik-Sikorska et al. (2021) ().…”

Section: Methodsmentioning

confidence: 99%

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Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens

Gbylik-Sikorska¹,

Łebkowska‐Wieruszewska²,

Gajda³

et al. 2021

Poultry Science

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Regardless of whether antimicrobial drugs are administered to laying hens legally or illegally, residues of these drugs may be present in the eggs. Even if the eggs are not intended for human consumption, byproducts/biowaste, such as eggshells, may contain residues of the drugs used, which may pose a risk to human health and the environment. In the presented research, 2 different groups of laying hens received enrofloxacin (10 mg/kg body weight) and lincomycin (20 mg/kg body weight) once daily for 5 d. Eggs were collected daily and the concentration of enrofloxacin, its metabolite ciprofloxacin, and lincomycin residue in the eggshells, whole eggs, egg yolks, and egg whites were determined by ultra-high-performance liquid chromatography-tandem mass spectrometry. This study demonstrates the transfer of enrofloxacin, ciprofloxacin, and lincomycin into the eggshells and provides evidence for the distribution into the eggshells after administration of these drugs to laying hens. The enrofloxacin residues were detected in the eggshell for 10 d after cessation of treatment, ciprofloxacin and lincomycin were rapidly eliminated and 2 d after finish drugs administration they were no longer detected in the eggshell.

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“…The LC-MS/MS analysis for all matrices was performed using the analytical method previously described by Gbylik-Sikorska et al. (2021) ().…”

Section: Methodsmentioning

confidence: 99%

“…The concentrations of ENR, CIP, and LIN in the eggshells were determined using a previously reported sample preparation procedure ( Gbylik-Sikorska et al., 2021 ). Sample preparation of the whole egg, egg yolk, and egg white was performed using a method described by Błądek et al.…”

Section: Methodsmentioning

confidence: 99%

Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens

Gbylik-Sikorska¹,

Łebkowska‐Wieruszewska²,

Gajda³

et al. 2021

Poultry Science

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“…There are many methods of detecting heavy metals, antibiotics, pesticides, and toxins, such as gas chromatography-mass spectrometry, 12,13 liquid chromatography-mass spectrometry, [14][15][16][17][18][19][20][21] inductively coupled plasma mass spectrometry, 22 graphite furnace atomic absorption spectrometry, 23 and electrochemical sensors 24,25 . All of these methods can offer accurate and sensitive results for contamination analysis.…”

Section: Introductionmentioning

confidence: 99%

Research progress on photoelectrochemical sensors for contamination analysis in agricultural fields

et al. 2022

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The establishment of rapid and efficient contamination detection methods in agricultural fields is of great significance for safeguarding agricultural production and human health, and for ensuring the healthy development of agricultural industries. As a booming analytical method, photoelectrochemical (PEC) sensors, which combine the advantages of electrochemical analysis and optical analysis, have received increasing research attention. Due to the complete separation of the excitation source (light) and the detection signal (current), the background signal of PEC sensors is greatly decreased, and their sensitivity is high. This review aims to cover the advances in PEC sensors for contamination analysis in agricultural fields over the past five years, including analysis of heavy metals, antibiotics, pesticides, and toxins. The future prospects in this field are also discussed.

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“…In recent decades, several essential methods have been applied for the detection of penicillin G, such as thin-layer chromatography (TLC) [4], immunoassays [5], gas chromatography-mass spectrometry (GC-MS) [6] and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [7]. Furthermore, compared to GC-MS and LC-MS/MS, TLC is relatively simple and inexpensive, but it is not very sensitive and efficient and cannot be used to analyze complicated substances [4].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, compared to GC-MS and LC-MS/MS, TLC is relatively simple and inexpensive, but it is not very sensitive and efficient and cannot be used to analyze complicated substances [4]. GC-MS and LC-MS/MS separate penicillin G from sophisticated sample matrices due to the high resolution of the column, and both could precisely obtain the target for quantitation [6,7]. Additionally, the LC-MS/MS method also have the ability for qualification to detect the analyte.…”

Section: Introductionmentioning

confidence: 99%

Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs

Liu

Guo

Wang

et al. 2021

Foods

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A simple and sensitive gas chromatography–tandem mass spectrometry (GC–MS/MS) method was established for the quantitative screening of penicillin G residues in chicken and duck eggs (whole egg, yolk and albumen). The analyte was separated on a TG-1MS capillary column (30.0 m × 0.25 mm i.d., 0.25 μm) with an external calibration method and electron impact (EI) ionization. Samples were pretreated using an accelerated solvent extraction (ASE) procedure followed by solid-phase extraction (SPE) on HLB cartridges (60 mg/3 mL). The derivative, which was safer and easier to store than penicillin G, was obtained by reacting trimethylsilyl diazomethane (TMSD) with penicillin G. The method was validated by the following parameters: linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The matrix-matched calibration curves had good linearity (R2 ≥ 0.9994) within the concentration range of LOQ–200.0 µg/kg for penicillin G in the sample matrices. In the same concentration range, the accuracy, in terms of recovery, was 80.31–94.50%; the relative standard deviation (RSD), intra-day RSD and inter-day RSD ranged from 1.24 to 3.44%, 2.13 to 4.82% and 2.74 to 6.13%, respectively. The LODs and LOQs of penicillin G in the matrices were in the ranges of 1.70–3.20 and 6.10–8.50 μg/kg, respectively. The applicability of the GC–MS/MS method was demonstrated by the determination of poultry eggs obtained from local markets with no penicillin G residues.

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Multi-Class Procedure for Analysis of 50 Antibacterial Compounds in Eggshells Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Cited by 5 publications

References 31 publications

Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens

Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens

Research progress on photoelectrochemical sensors for contamination analysis in agricultural fields

Establishment and Validation of a GC–MS/MS Method for the Quantification of Penicillin G Residues in Poultry Eggs

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