Multi-class methodology to determine pesticides and mycotoxins in green tea and royal jelly supplements by liquid chromatography coupled to Orbitrap high resolution mass spectrometry

Martínez-Domínguez, Gerardo; Romero‐González, Roberto

doi:10.1016/j.foodchem.2015.11.070

Cited by 113 publications

(64 citation statements)

References 26 publications

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“…Nevertheless, nutraceutical products are not considered by the legislation yet but, due to a potential carryover during the manufacturing process, contamination could be expected not only in raw material, but also in other by-products. Moreover, several studies have reported the sole presence of pesticides [21,22], mycotoxins [23,24] and both types of contaminants [25][26][27] in diverse food supplements, remarking the necessity to evaluate the contamination profile of these products considering their rising consumption and popularity.…”

Section: Introductionmentioning

confidence: 99%

“…Concerning the extraction of contaminants, QuEChERS (quick, easy, cheap, effective, rugged and safe) [21,23,24] and "dilute and shoot" procedures have been recently applied to food supplements delivered as gelatin capsules, traditional capsules, tablets, powder extracts or liquid presentations [25][26][27]. Analytical methods used in the detection and quantification of contamination include ELISA detection [28], gas chromatography (GC) coupled with mass spectrometry (MS) [22] and ultra-high-performance liquid chromatography (UHPLC) coupled with tandem mass spectrometry (MS/MS) [23,24] and high-resolution Orbitrap mass spectrometry (Q-Orbitrap HRMS) [25][26][27]. Due to its high resolving power, sensitivity and accurate mass measurement, high-resolution mass spectrometry stands as a suitable alternative for evaluating a large number of contaminants present in complex matrices at low concentrations.…”

Section: Introductionmentioning

confidence: 99%

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Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Orbitrap High-Resolution Mass Spectrometry for Multi-Residue Analysis of Mycotoxins and Pesticides in Botanical Nutraceuticals

Narváez

Rodríguez-Carrasco

Castaldo

et al. 2020

Toxins

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Cannabidiol (CBD) food supplements made of Cannabis sativa L. extracts have quickly become popular products due to their health-promoting effects. However, potential contaminants, such as mycotoxins and pesticides, can be coextracted during the manufacturing process and placed into the final product. Accordingly, a novel methodology using ultra-high-performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) was developed to quantify 16 mycotoxins produced by major C. sativa fungi, followed by a post-target screening of 283 pesticides based on a comprehensive spectral library. The validated procedure was applied to ten CBD-based products. Up to six different Fusarium mycotoxins were found in seven samples, the most prevalent being zearalenone (60%) and enniatin B1 (30%), both found at a maximum level of 11.6 ng/g. Co-occurrence was observed in four samples, including one with enniatin B1, enniatin A and enniatin A1. On the other hand, 46 different pesticides were detected after retrospective analysis. Ethoxyquin (50%), piperonyl butoxide (40%), simazine (30%) and cyanazine (30%) were the major residues found. These results highlight the necessity of monitoring contaminants in food supplements in order to ensure a safe consumption, even more considering the increase trend in their use. Furthermore, the developed procedure is proposed as a powerful analytical tool to evaluate the potential mycotoxin profile of these particular products.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Orbitrap High-Resolution Mass Spectrometry for Multi-Residue Analysis of Mycotoxins and Pesticides in Botanical Nutraceuticals

Narváez

Rodríguez-Carrasco

Castaldo

et al. 2020

Toxins

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“…Thus, this formation is a central part of proto-filaments, whereas the rest of the protein remains unfolded outside of the main axis [16]. The Q-exactive focus is a newly released hybrid high resolution mass spectrometer used for metabolomics analysis including pesticides, herbicides, drugs, antibiotics, small peptides and several other organic molecules [17,18,19,20,21]. The hyphenated Q-exactive focus instrument combines ultra high pressure liquid chromatography coupled with photodiode array detector (UHPLC-PDA) with an orbital trap and a high-resolution collision cell, which allows high resolution MS n fragments [17,18,19,20,21].…”

Section: Introductionmentioning

confidence: 99%

Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor

Cornejo

Salgado

Caballero

et al. 2016

IJMS

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Liquid chromatography coupled with mass spectrometry is an outstanding methodology for fast analysis of phenolic compounds in biological samples. Twenty two compounds were quickly and accurately identified in the methanolic extract of the Antarctic lichen Ramalina terebrata for the first time using ultra high pressure liquid chromatography coupled with photodiode array detector and high resolution mass spectrometry (UHPLC-PDA-Q/Orbitrap/MS/MS). In addition, the extract and the four compounds isolated from this species were tested for the inhibitory activity of tau protein aggregation, which is a protein involved in Alzheimer’s disease (AD). All compounds showed null activity with the exception of parietin, which it was able to inhibit aggregation process of tau in a concentration range between 3 µg/mL (10 µM) to 28 µg/mL (100 µM). In addition, we show how parietin interact with tau 306VQIVYK311 hexapeptide inside of the microtubule binding domain (4R) with the help of molecular docking experiments. Finally, the constituents present in the methanolic extract could possibly contribute to the established anti-aggregation activity for this extract and this in-depth analysis of the chemical composition of R. terebrata could guide further research into its medicinal properties and potential uses.

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“…Among three major nonalcoholic drinks (tea, coffee, and cocoa) worldwide, tea is the most widely consumed beverage for both pleasure and therapeutic purposes [1][2][3]. During the Article Related Abbreviations: d-SPE, dispersive solid-phase extraction; EDR, extended dynamic range; EIC, extracted ion chromatogram; GCB, graphitized carbon black; HRMS, high resolution mass spectrometry; LOI, limit of identification; ME, matrix effect; MRL, the maximum residue limit; MRM, multiple reaction monitoring; MWCNT, multiwalled carbon nanotube; n-MEW, narrow mass extraction window; PSA, primary secondary amine.…”

Section: Introductionmentioning

confidence: 99%

Determination of multiple pesticide residues in teas by gas chromatography with accurate time‐of‐flight mass spectrometry

Teng

Wang³

et al. 2019

J of Separation Science

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In this work, gas chromatography tandem with electron ionization and full-scan high-resolution mass spectrometry with a time-of-flight mass analyzer was evaluated for analyzing pesticide residues in teas. The relevant aspects for mass spectrometry analysis, including the resolution and mass accuracy, acquisition rate, temperature of ion source, were investigated. Under acquisition condition in 2-GHz extended dynamic range mode, accurate mass spectral library including 184 gas chromatography detectable pesticides was established and retrieval parameters were optimized.The mass spectra were consistent over a wide concentration range (three orders) with good match values to those of NIST (EI-quadrupole). The methodology was verified by the validation of 184 pesticides in four tea matrices. A wide linear range (1-1000 μg/kg) was obtained for most compounds in four matrices. Limit of detection, limit of quantification, and limit of identification values acquired in this study could satisfy the requirements for maximum residue levels prescribed by the European Community. Recovery studies were performed at three concentrations (10, 50, and 100 μg/kg). Most of the analytes were recovered at an acceptable range of 70-120% with relative standard deviations ≤ 20% in four matrices. The potential extension of qualitative screening scope makes gas chromatography tandem with electron ionization and mass spectrometry with a time-of-flight mass analyzer a more powerful tool compared with gas chromatography with tandem mass spectrometry. K E Y W O R D Saccurate mass spectral library, gas chromatography, pesticides, teas, time-of-flight mass analyzer 1990

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Multi-class methodology to determine pesticides and mycotoxins in green tea and royal jelly supplements by liquid chromatography coupled to Orbitrap high resolution mass spectrometry

Cited by 113 publications

References 26 publications

Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Orbitrap High-Resolution Mass Spectrometry for Multi-Residue Analysis of Mycotoxins and Pesticides in Botanical Nutraceuticals

Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Orbitrap High-Resolution Mass Spectrometry for Multi-Residue Analysis of Mycotoxins and Pesticides in Botanical Nutraceuticals

Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor

Determination of multiple pesticide residues in teas by gas chromatography with accurate time‐of‐flight mass spectrometry

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