Multi-channeled biodegradable polymer/CultiSpher composite nerve guides

Bender, Matthew D.; Bennett, Jennifer M.; Waddell, Rebecca; Doctor, John S.; Marra, Kacey G.

doi:10.1016/j.biomaterials.2003.08.046

Cited by 90 publications

(79 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, bridges with multiple channels have been developed with the objective of maintaining a path for regrowing axons, which may also orient axonal elongation. 6,[18][19][20][21][22] Channels with diameters ranging from 40 to 600 mm have been fabricated for spinal cord regeneration, [18][19][20] though the correlation between channel diameter and regeneration has not previously been determined.…”

mentioning

confidence: 99%

Multiple Channel Bridges for Spinal Cord Injury: Cellular Characterization of Host Response

Yang

Laporte

Zelivyanskaya

et al. 2009

Tissue Engineering Part A

View full text Add to dashboard Cite

Bridges for treatment of the injured spinal cord must stabilize the injury site to prevent secondary damage and create a permissive environment that promotes regeneration. The host response to the bridge is central to creating a permissive environment, as the cell types that respond to the injury have the potential to secrete both stimulatory and inhibitory factors. We investigated multiple channel bridges for spinal cord regeneration and correlated the bridge structure to cell infiltration and axonal elongation. Poly(lactide-co-glycolide) bridges were fabricated by a gas foaming=particulate leaching process. Channels within the bridge had diameters of 150 or 250 mm, and the main body of the bridge was highly porous with a controllable pore size. Upon implantation in a rat spinal cord hemisection site, cells infiltrated into the bridge pores and channels, with the pore size influencing the rate of infiltration. The pores had significant cell infiltration, including fibroblasts, macrophages, S-100b-positive cells, and endothelial cells. The channels of the bridge were completely infiltrated with cells, which had aligned axially, and consisted primarily of fibroblasts, S-100b-positive cells, and endothelial cells. Reactive astrocytes were observed primarily outside of the bridge, and staining for chondroitin sulfate proteoglycans was decreased in the region surrounding the bridge relative to studies without bridges. Neurofilament staining revealed a preferential growth of the neural fibers within the bridge channels relative to the pores. Multiple channel bridges capable of supporting cellular infiltration, creating a permissive environment, and directing the growth of neural fibers have potential for promoting and directing spinal cord regeneration.

show abstract

mentioning

confidence: 99%

Multiple Channel Bridges for Spinal Cord Injury: Cellular Characterization of Host Response

Yang

Laporte

Zelivyanskaya

et al. 2009

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Earlier results have suggested that a higher degree of PC12 cell proliferation is observed with the other hydrogels. [17][18][19][20] These results contradict the conventional idea that peptide hydrogels might not provide a favorable environment for proliferation of PC12 cells. However, the mechanism by which the SF16 peptide hydrogel environment promoted cell proliferation is complex and requires further study.…”

Section: D Cell Culturementioning

confidence: 57%

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Wei¹,

Yao²,

Wang³

et al. 2013

IJN

View full text Add to dashboard Cite

Background: Peripheral nerve injury is a common trauma, but presents a significant challenge to the clinic. Silk-based materials have recently become an important biomaterial for tissue engineering applications due to silk's biocompatibility and impressive mechanical and degradative properties. In the present study, a silk fibroin peptide (SF16) was designed and used as a component of the hydrogel scaffold for the repair of peripheral nerve injury. Methods: The SF16 peptide's structure was characterized using spectrophotometry and atomic force microscopy, and the SF16 hydrogel was analyzed using scanning electron microscopy. The effects of the SF16 hydrogel on the viability and growth of live cells was first assessed in vitro, on PC12 cells. The in vivo test model involved the repair of a nerve gap with tubular nerve guides, through which it was possible to identify if the SF16 hydrogel would have the potential to enhance nerve regeneration. In this model physiological saline was set as the negative control, and collagen as the positive control. Walking track analysis and electrophysiological methods were used to evaluate the functional recovery of the nerve at 4 and 8 weeks after surgery. Results: Analysis of the SF16 peptide's characteristics indicated that it consisted of a welldefined secondary structure and exhibited self-assembly. Results of scanning electron microscopy showed that the peptide based hydrogel may represent a porous scaffold that is viable for repair of peripheral nerve injury. Analysis of cell culture also supported that the hydrogel was an effective matrix to maintain the viability, morphology and proliferation of PC12 cells. Electrophysiology demonstrated that the use of the hydrogel scaffold (SF16 or collagen) resulted in a significant improvement in amplitude recovery in the in vivo model compared to physiological saline. Moreover, nerve cells in the SF16 hydrogel group displayed greater axon density, larger average axon diameter and thicker myelin compared to those of the group that received physiological saline. Conclusion: The SF16 hydrogel scaffold may promote excellent axonal regeneration and functional recovery after peripheral nerve injury, and the SF16 peptide may be a candidate for nerve tissue engineering applications.

show abstract

“…Examples include poly (L-lactic acid) (PLLA), poly (lactic acid-e-caprolactone), poly (L-lactideco-glycolide), poly (1, 3-trimethylenecarbonate-e-caprolactone) and poly (caprolactone) (PCL). 22,130,131,155,[163][164][165][166]168 Copolymerization has been used to obtain these materials with characteristics in terms of tailored to degradation behavior, mechanical performance, thermal properties and wettability. The repair of clinically relevant nerve defects generally requires a period of up to one year for nerve regeneration and maturation.…”

Section: Biodegradable Synthetic Materialsmentioning

confidence: 99%

Regeneration and repair of peripheral nerves with different biomaterials: Review

2010

View full text Add to dashboard Cite

Peripheral nerve injury may cause gaps between the nerve stumps. Axonal proliferation in nerve conduits is limited to 10-15 mm. Most of the supportive research has been done on rat or mouse models which are different from humans. Herein we review autografts and biomaterials which are commonly used for nerve gap repair and their respective outcomes. Nerve autografting has been the first choice for repairing peripheral nerve gaps. However, it has been demonstrated experimentally that tissue engineered tubes can also permit lead to effective nerve repair over gaps longer than 4 cm repair that was previously thought to be restorable by means of nerve graft only. All of the discoveries in the nerve armamentarium are making their way into the clinic, where they are, showing great potential for improving both the extent and rate of functional recovery compared with alternative nerve guides.

show abstract

Multi-channeled biodegradable polymer/CultiSpher composite nerve guides

Cited by 90 publications

References 25 publications

Multiple Channel Bridges for Spinal Cord Injury: Cellular Characterization of Host Response

Multiple Channel Bridges for Spinal Cord Injury: Cellular Characterization of Host Response

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Regeneration and repair of peripheral nerves with different biomaterials: Review

Contact Info

Product

Resources

About