Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery

Wu, Shihua; Yang, Lu; Gao, Yuan; Liu, Xiaoyue; Liu, Feiyan

doi:10.1016/j.chroma.2007.12.024

Cited by 61 publications

(49 citation statements)

References 57 publications

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“…In spite of the harmful effects of invasiveness to many ecosystems, goldenrod is permitted for use as a herbal medicine ingredient in several countries [21][22][23]. In others, goldenrod and its related species are used as one of the popular cutflower species, and an efficient in vitro propagation method for valuable clones could be of significant commercial value.…”

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confidence: 99%

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Kang

Sheng

et al. 2012

Acta Soc Bot Pol

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Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog’s medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed

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confidence: 99%

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Kang

Sheng

et al. 2012

Acta Soc Bot Pol

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“…One is multi-channel CCC device recently developed by our group [6,9,40]. It has three column holders with 10 cm of revolution radius.…”

Section: And Hplc Apparatusmentioning

confidence: 99%

“…For targeted separation of tanshinones from S. miltiorrhiza Bunge (100 mg of sample was dissolved in 20 mL solvent system each injection), a three-channel CCC [6,9,40] was used. The instrument ran at 500 rpm and the retention of stationary phase was controlled at 60%.…”

Section: Targeted CCC Separation Proceduresmentioning

confidence: 99%

A novel 9×9 map-based solvent selection strategy for targeted counter-current chromatography isolation of natural products

Liang

Meng

et al. 2015

Journal of Chromatography A

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“…Undoubtedly, HSCCC has great potential in drug discovery by providing ideal fractionation of complex natural products, with 100% theoretical sample recovery. As a matter of fact, HSCCC has been successfully used as such a tool in several efforts to find bioactive compounds from herbal medicines [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%