Multi-chamber microfluidic platform for high-precision skin permeation testing

Alberti, Massimo; Dancik, Yuri; Sriram, Gopu; Wu, Bo; Teo, Yi Ling; Feng, Z.; Bigliardi-Qi, Mei; Wu, Ruige; Wang, Z. P.; Bigliardi, Paul L.

doi:10.1039/c6lc01574c

Cited by 58 publications

(42 citation statements)

References 46 publications

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“…This gut-on-a-chip device, along with other intestinal chips such as HuMiX, has been used to recapitulate the interplay between intestinal microbes and the epithelium (Kim et al, 2015; Shah et al, 2016). Innovative perfusable vascularized skin-on-a-chip models (Wufuer et al, 2016; Mori et al, 2017) or immune-competent models (Ramadan and Ting, 2016) have been proposed, both aiming to create more physiologically relevant skin equivalents for drug screening and disease modeling (Abaci et al, 2015; Alberti et al, 2017; van den Broek et al, 2017; Sriram et al, 2018).…”

Section: Lab-on-a-chip Devices Mimicking Epithelial Tissuesmentioning

confidence: 99%

Mimicking Epithelial Tissues in Three-Dimensional Cell Culture Models

Torras

García-Díaz

Fernández‐Majada

et al. 2018

Front. Bioeng. Biotechnol.

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Epithelial tissues are composed of layers of tightly connected cells shaped into complex three-dimensional (3D) structures such as cysts, tubules, or invaginations. These complex 3D structures are important for organ-specific functions and often create biochemical gradients that guide cell positioning and compartmentalization within the organ. One of the main functions of epithelia is to act as physical barriers that protect the underlying tissues from external insults. In vitro, epithelial barriers are usually mimicked by oversimplified models based on cell lines grown as monolayers on flat surfaces. While useful to answer certain questions, these models cannot fully capture the in vivo organ physiology and often yield poor predictions. In order to progress further in basic and translational research, disease modeling, drug discovery, and regenerative medicine, it is essential to advance the development of new in vitro predictive models of epithelial tissues that are capable of representing the in vivo-like structures and organ functionality more accurately. Here, we review current strategies for obtaining biomimetic systems in the form of advanced in vitro models that allow for more reliable and safer preclinical tests. The current state of the art and potential applications of self-organized cell-based systems, organ-on-a-chip devices that incorporate sensors and monitoring capabilities, as well as microfabrication techniques including bioprinting and photolithography, are discussed. These techniques could be combined to help provide highly predictive drug tests for patient-specific conditions in the near future.

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Section: Lab-on-a-chip Devices Mimicking Epithelial Tissuesmentioning

confidence: 99%

Mimicking Epithelial Tissues in Three-Dimensional Cell Culture Models

Torras

García-Díaz

Fernández‐Majada

et al. 2018

Front. Bioeng. Biotechnol.

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“…Liu et al for instance looked at the clearance rates of testosterone in a coculture of hepatocytes on electrospun fibers . Alberti et al meanwhile studied the penetration of testosterone through organotypic skin . Using explant cultures of rodent and human adipose tissue, further in vitro studies demonstrated the reduction of proinflammatory cytokines due to estrogen as well as sex differences in both spontaneous and estrogen‐stimulated leptin secretion …”

Section: Sex‐differences In Nonfemale‐specific Organ‐on‐a‐chip Systemsmentioning

confidence: 99%

Organ‐on‐a‐Chip Systems for Women's Health Applications

Nawroth

Rogal

Weiß

et al. 2017

Adv Healthcare Materials

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Biomedical research, for a long time, has paid little attention to the influence of sex in many areas of study, ranging from molecular and cellular biology to animal models and clinical studies on human subjects. Many studies solely rely on male cells/tissues/animals/humans, although there are profound differences in male and female physiology, which can significantly impact disease mechanisms, toxicity of compounds, and efficacy of pharmaceuticals. In vitro systems have been traditionally very limited in their capacity to recapitulate female-specific physiology and anatomy such as dynamic sex-hormone levels and the complex interdependencies of female reproductive tract organs. However, the advent of microphysiological organ-on-a-chip systems, which attempt to recreate the 3D structure and function of human organs, now gives researchers the opportunity to integrate cells and tissues from a variety of individuals. Moreover, adding a dynamic flow environment allows mimicking endocrine signaling during the menstrual cycle and pregnancy, as well as providing a controlled microfluidic environment for pharmacokinetic modeling. This review gives an introduction into preclinical and clinical research on women's health and discusses where organ-on-a-chip systems are already utilized or have the potential to deliver new insights and enable entirely new types of studies.

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“…Skin-on-chip devices offer innovative and state-of-the-art platforms essential to overcome the above-mentioned limitations of Franz diffusion cells [10]. In some studies, organotypic cultures are cultivated in the chips to mimic human skin and the transepithelial electrical resistance (TEER) is detected [11,12,13] for characterization the diffusion properties. The tissue vascularization can also be considered in the preparation of skin cell containing co-cultures [14].…”

Section: Introductionmentioning

confidence: 99%

Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs—Design, Fabrication, and Testing

et al. 2019

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To develop proper drug formulations and to optimize the delivery of their active ingredients through the dermal barrier, the Franz diffusion cell system is the most widely used in vitro/ex vivo technique. However, different providers and manufacturers make various types of this equipment (horizontal, vertical, static, flow-through, smaller and larger chambers, etc.) with high variability and not fully comparable and consistent data. Furthermore, a high amount of test drug formulations and large size of diffusion skin surface and membranes are important requirements for the application of these methods. The aim of our study was to develop a novel Microfluidic Diffusion Chamber device and compare it with the traditional techniques. Here the design, fabrication, and a pilot testing of a microfluidic skin-on-a chip device are described. Based on this chip, further developments can also be implemented for industrial purposes to assist the characterization and optimization of drug formulations, dermal pharmacokinetics, and pharmacodynamic studies. The advantages of our device, beside the low costs, are the small drug and skin consumption, low sample volumes, dynamic arrangement with continuous flow mimicking the dermal circulation, as well as rapid and reproducible results.

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Multi-chamber microfluidic platform for high-precision skin permeation testing

Cited by 58 publications

References 46 publications

Mimicking Epithelial Tissues in Three-Dimensional Cell Culture Models

Mimicking Epithelial Tissues in Three-Dimensional Cell Culture Models

Organ‐on‐a‐Chip Systems for Women's Health Applications

Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs—Design, Fabrication, and Testing

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