Multi-chamber microcapsule generation for cell culture using a high- and low-volume centrifuge-based droplet shooting device

Petersen, Kevin E.; Asangba, Abigail E.

doi:10.1007/s10404-023-02651-6

Cited by 1 publication

(1 citation statement)

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“…The cells in the logarithmic growth phase were utilized [38,39]. The original culture medium plus PBS buffer was discarded, and the cells were washed, digested with trypsin and centrifuged to discard the supernatant at the end of digestion.…”

Section: Cell Proliferation Assay Via Mtt Methodsmentioning

confidence: 99%

Mechanistic and Functional Studies on the Microbial Induction of Wolfiporia cocos Liquid Fermentation Products

Yang,

Su,

et al. 2024

Foods

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Liquid fermentation is an efficient culture for obtaining polysaccharides from edible mushrooms. In this study, the polysaccharide content and biomass were examined by introducing microorganisms into the Wolfiporia cocos fermentation system. Three edible mushroom co-fermentation systems were established, among which the Wolfiporia cocos-Ganoderma lucidum co-fermentation system significantly increased the mycelial biomass of the system by 57.71% compared to Wolfiporia cocos alone and 91.22% compared to Ganoderma lucidum alone, and the intracellular polysaccharide content was significantly increased. Physiological activities of polysaccharides showed that mycelial polysaccharides in the Wolfiporia cocos-Ganoderma lucidum system had stronger anti-tumor cell value-adding and anti-tumor cell migration activities compared with Wolfiporia cocos and Ganoderma lucidum fermentation alone. The transcriptomic study of Wolfiporia cocos mycelium induced by exogenous substances suggested that the exogenous substances could enhance the intracellular polysaccharide content of Wolfiporia cocos through the upregulation of the expression of α-glycosyltransferase encoded by ALG10 and the downregulation of α-glycosidases encoded by MAN1B in the glycolytic metabolism of Wolfiporia cocos. This study provides a new direction for the transformation of polysaccharides from Wolfiporia cocos and Ganoderma lucidum into functional foods and new product development, and provides an experimental basis.

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Section: Cell Proliferation Assay Via Mtt Methodsmentioning

confidence: 99%