Multi-Carbon Dots and Aptamer Based Signal Amplification Ratiometric Fluorescence Probe for Protein Tyrosine Kinase 7 Detection

Abstract

Background: Protein tyrosine kinase 7 (PTK 7) is a membrane receptor, whichcan be found in various kinds of cancers . In view of this, detection of PTK 7 in theperipheral circulation would be an effective way for the early diagnosis of cancer.Results: In this work, a multi carbon dots and aptamer based signal amplificationratiometric fluorescence probe was developed . The fluorescence of the aptamermodified y CDs and b CDs were respectively chosen as the detection signal and interiorlabel The fluorescence of y CDs was quenched by Fe 3 O 4 and cDNA (complement toaptamer) compound without PTK 7 but recovered by the addition of PTK 7. Then, t hefree aptamer was cut by DNase Ⅰ, which amplified the detection signal. The ratiometricfluorescence sensor for PTK 7 was established with the LOD of 0.016 ng mL-1Conclusions: Summary , a multi carbon dots and aptamer based signal amplificationratiometric fluorescence probe was developed for the detection of protein tyrosinekinase 7. The developed probe was applied to PTK 7 detection in MCF 7 cells andhuman serum with satisf ying results, thus indicat ing that this probe ha s huge potentialin clinical practice.