Multi-biomarker approach to assess chromium, pH and temperature toxicity in fish

Kumar, Neeraj; Bhushan, Shashi; Patole, Pooja Bapurao; Gite, Archana

doi:10.1016/j.cbpc.2021.109264

Cited by 8 publications

(10 citation statements)

References 69 publications

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“…The diverse biomarkers were categorized into distinct physiological functions, encompassing oxidative stress, cytotoxicity, neuro‐signaling, metabolic stress, stress proteins, stress hormones, immunity, and detoxification. These physiological functions collectively serve as reliable biomarkers in aquatic animals, including fish 54,110 . Neuro‐signaling emerges as a potent biomarker for contamination in aquatic ecosystems, with other biomarkers also contributing to the suite of pollution indicators.…”

Section: Resultsmentioning

confidence: 99%

“…The assessment of DNA damage was conducted using the single‐cell gel electrophoresis (SCGE) method (alkaline single‐cell gel electrophoresis/comet assay) in gill tissue, employing a three‐layer agarose approach as described by Ali and colleagues 53 with slight modifications 54 . To outline the process briefly, gill tissues (50 mg) were initially cleansed with double distilled water, then twice with chilled phosphate‐buffered saline (Ca 2+ Mg 2+ free), and subsequently transferred to an ice‐cold homogenization buffer (20 mM EDTA; 1‐X Hanks' balanced salt solution; 10% dimethyl sulfoxide [DMSO], pH 7.0–7.5).…”

Section: Methodsmentioning

confidence: 99%

“…The assessment of DNA damage was conducted using the single-cell gel electrophoresis (SCGE) method (alkaline single-cell gel electrophoresis/comet assay) in gill tissue, employing a three-layer agarose approach as described by Ali and colleagues 53 with slight modifications. 54 To outline the process briefly, gill tissues (50 mg)…”

Section: Alkaline Single-cell Gel Electrophoresis/ Comet Assaymentioning

confidence: 99%

“…These physiological functions collectively serve as reliable biomarkers in aquatic animals, including fish. 54,110 Neurosignaling emerges as a potent biomarker for contamination in aquatic ecosystems, with other biomarkers also contributing to the suite of pollution indicators. tions.…”

Section: Histopathological Studymentioning

confidence: 99%

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Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Kumar,

Gismondi,

Reddy

2023

Environmental Toxicology

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The current study focused on assessing the toxicological effects of copper (Cu) and copper nanoparticles (Cu‐NPs) in acute condition on Pangasianodon hypophthalmus. The median lethal concentration (LC50) for Cu and Cu‐NPs were determined as 8.04 and 3.85 mg L−1, respectively. For the subsequent definitive test, varying concentrations were selected: 7.0, 7.5, 8.0, 8.5, and 9.0 mg L−1 for Cu, and 3.0, 3.3, 3.6, 3.9, and 4.2 mg L−1 for Cu‐NPs. To encompass these concentration levels and assess their toxic effects, biomarkers associated with toxicological studies like oxidative stress, neurotransmission, and cellular metabolism were measured in the liver, kidney, and gill tissues. Notably, during the acute test, the activities of catalase, superoxide dismutase, glutathione‐s‐transferase, glutathione peroxidase, and lipid peroxide in the liver, gill, and kidney tissues were significantly increased due to exposure to Cu and Cu‐NPs. Similarly, acetylcholinesterase activity in the brain was notably inhibited in the presence of Cu and Cu‐NPs when compared to the control group. Cellular metabolic stress was greatly influenced by the exposure to Cu and Cu‐NPs, evident from the considerable elevation of cortisol, HSP 70, and blood glucose levels in the treated groups. Furthermore, integrated biomarker response, genotoxicity, DNA damage in gill tissue, karyotyping in kidney tissue, and histopathology in gill and liver were investigated, revealing tissue damage attributed to exposure to Cu and Cu‐NPs. In conclusion, this study determined that elevated concentrations of essential trace elements, namely Cu and Cu‐NPs, induce toxicity and disrupt cellular metabolic activities in fish.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Alkaline Single-cell Gel Electrophoresis/ Comet Assaymentioning

confidence: 99%

Section: Histopathological Studymentioning

confidence: 99%

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Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Kumar,

Gismondi,

Reddy

2023

Environmental Toxicology

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“…In the present study, the bioaccumulation of Cr in muscle, liver, gill, kidney, and brain was below 1.8 mg kg −1 except in the fish muscle site 11. USFDA 59 states that the MRL level for Cr should be not more than 12–13 mg kg −1 , and the requirement for humans is 1 µg day −1 . However, Cr elements are necessary for metabolic purposes in limited quantities.…”

Section: Discussionmentioning

confidence: 99%

Health risk assessment and metal contamination in fish, water and soil sediments in the East Kolkata Wetlands, India, Ramsar site

Kumar

Chandan

Bhushan

et al. 2023

Sci Rep

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East Kolkata Wetlands (EKW) is an important site for fish culture in sewage-fed areas, which are major receivers of pollutants and wastages from Kolkata. EKW is internationally important as the Ramsar site was declared on Aug 2002 with an area of 125 km2. EKW is a natural water body where wastewater-fed natural aquaculture has been practiced for more than 70 years. It is ecologically vulnerable due to the discharge of toxic waste through sewage canals from cities. Assessing the EKW to understand the inflow and load of the toxic metal (s) in fish, water, and sediments samples is essential. The field (samples collection from 13 sites) and lab (determination of toxic level of metals) based research were carried out to assess metal toxicity and health risk assessment in EKW. The levels of eighteen metals (18), namely Chromium, Vanadium, Cobalt, Manganese, Copper, Nickel, Zinc, Silver, Molybdenum, Arsenic, Selenium, Tin, Gallium, Germanium, Strontium, Cadmium, Mercury, and Lead, were determined using Inductively coupled plasma mass spectrometry (ICP-MS) in five fish tissues viz. muscle, liver, kidney, gill and brain, along with the water samples and soil sediments in 13 sampling sites. The bioaccumulation and concentration of metals in fish tissues, soil sediments, and water samples were well within the safe level concerning the recommendation of different national and international agencies except for a few metals in a few sampling sites like Cd, As, and Pb. The geoaccumulation index (Igeo) was also determined in the soil sediments, indicating moderate arsenic, selenium, and mercury contamination in a few sites. The contamination index in water was also determined in 13 sampling sites. The estimated daily intake (EDI), reference dose (RfD), target hazard quotient (THQ), slope factor and cancer risk of Cr, Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb and Hg from fish muscle were determined. Based on the results of the present investigation, it is concluded that fish consumption in the East Kolkata Wetland (EKW) is safe. The effects of bioaccumulation of metals in muscle tissue were well within the safe level for consumption as recommended by WHO/FAO.

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Effect of dietary inclusion of Pennisetum purpureum (Napier) grass on growth performance, rumen fermentation and meat quality of feedlot sussex red steers

Rabatseta,

Fourie,

Nkosi

et al. 2024

Trop Anim Health Prod

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The aim of this study was to evaluate the growth performance, fermentation indices and meat quality of Sussex steers fed totally mixed rations that composed of graded inclusion levels of Napier grass (NP). Three experimental diets designated as diet 1 (0.0 g kg–1 NP: Control), diet 2 (300 g kg–1 NP grass) and diet 3 (600 g kg–1 NP) were formulated. Twenty-four male steers aged 8 months with an average body weight of 185.0 ± 30 kg were used. In a completely randomized design, the animals were allocated to the diets and fed for 120 days. Dietary NP inclusion reduced (P < 0.05) the animals’ average daily gain and increased the feed efficiency. The steers’ daily feed intake and final body weight decreased (P < 0.05) with a 600 g kg–1 inclusion level. The fermentation indices were not affected (P > 0.05) by the inclusion. While the inclusion reduced (P < 0.05) warm muscle temperature, it had no effect (P > 0.05) on carcass dressing percentage, warm and cold initial and ultimate pH. However, 600 g kg–1 inclusion level reduced (P > 0.05) warm and cold carcass weights. Meat physical attributes, moisture characteristics and tenderness were not affected (P > 0.05) by dietary treatments, except for the 7-days aged meat thaw loss, which increased at 600 g kg–1 inclusion level. Inclusion of 300 g kg–1 increased meat protein and fat, but dry and organic matter contents decreased with increasing inclusion levels. Dietary inclusion of NP grass up to 300 g kg–1 in steers’ diets improved feed intake, carcass traits and yielded meat high in protein and fat.

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Multi-biomarker approach to assess chromium, pH and temperature toxicity in fish

Cited by 8 publications

References 69 publications

Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Health risk assessment and metal contamination in fish, water and soil sediments in the East Kolkata Wetlands, India, Ramsar site

Effect of dietary inclusion of Pennisetum purpureum (Napier) grass on growth performance, rumen fermentation and meat quality of feedlot sussex red steers

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