Multi-barrelled ion-selective microelectrodes as tools for the investigation of volume regulation mechanisms in invertebrate nerve cells under hyperosmotic conditions

Dierkes, Paul Wilhelm; Neumann, Susanne; Klees, Guido; Schlue, Wolf-Rüdiger

doi:10.1016/s0013-4686(03)00407-9

Cited by 6 publications

(7 citation statements)

References 19 publications

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“…A similar behaviour has been shown previously for hippocampal neurons, which exhibited RVD only occasionally in slice preparations (Kreisman and Olson, 2003), and never in cultured neurons (Aitken et al, 1998). However, the volume changes of Retzius neurons upon changing the extracellular osmolality were distinctly smaller than expected for an ideal osmometer (Fig.·2B,D), suggesting that volume change and volume regulation occur more or less in parallel (Dierkes et al, 2002;Dierkes et al, 2003).…”

Section: Discussionsupporting

confidence: 84%

Swelling-activated chloride channels in leech Retzius neurons

Coulon

Wüsten

Hochstrate

et al. 2008

Journal of Experimental Biology

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SUMMARYDuring periods of high activity neurons are expected to swell due to the uptake of Cl -. To find out whether leech Retzius neurons possess swelling-activated Cl -channels that facilitate Cl -efflux and, hence, volume recovery, we exposed the cells to hypotonic solutions. In hypotonic solutions, the cells slowly swelled but did not undergo a regulatory volume decrease. However, the cell volume increased less than predicted for an ideal osmometer, suggesting the action of a compensatory mechanism. The cell swelling was paralleled by a marked decrease in the input resistance as well as by the activation of a membrane current with a reversal potential close to the Cl -equilibrium potential. This current was substantially diminished by removing bath Cl -, by applying the Cl -channel blocker DIDS, or by treating the cells with the tubulin polymerization inhibitor colchicine. Furthermore, in the presence of colchicine or vinblastine, the cell swelling was substantially increased. It is concluded that leech Retzius neurons possess swelling-activated Cl -channels that require an intact microtubule system for activation. The channels may help to restore cell volume after periods of high neuronal activity.

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Section: Discussionsupporting

confidence: 84%

Swelling-activated chloride channels in leech Retzius neurons

Coulon

Wüsten

Hochstrate

et al. 2008

Journal of Experimental Biology

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“…In leech neurons, TMA + has been found to be a suitable volume marker, whereby its concentration was measured by ionsensitive microelectrodes filled with Corning 477317 ( [15,16,35]; see [36]). However, when using this method, the experimental time was limited to about 30 min because of TMA + loss (see [35]).…”

Section: Resultsmentioning

confidence: 99%

“…If the cells behaved like ideal osmometers, this increase should cause a corresponding cell swelling, which is in reasonable accordance with the experimental data (Table 1; Figs. 3a and 4; see [16,41]). (3) In the absence of extracellular Na + or Cl − , i.e., under conditions which exclude NaCl uptake by the cell, ouabain had no effect on the cell volume (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The intracellular concentrations of Na + , K + , Cl − , or tetramethylammonium (TMA + , see "Monitoring of cytosolic volume by using Fura-2") were measured by triple-barrelled ion-sensitive microelectrodes (see [16]), which allowed for the simultaneous recording of two ion species together with E m . The two ion-sensitive barrels were filled with one of the following sensors:…”

Section: Ion-sensitive Microelectrodesmentioning

confidence: 99%

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Ionic mechanism of ouabain-induced swelling of leech Retzius neurons

Dierkes

Wüsten

Klees

et al. 2005

Pflugers Arch - Eur J Physiol

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By using electrophysiological and microfluorimetric methods, we found that leech Retzius neurons swell after inhibition of the Na(+)-K(+) pump by the cardiac glycoside ouabain. To explore the mechanism of this swelling, we measured the effect of ouabain on [Na(+)](i), [K(+)](i), and [Cl(-)](i), as well as on the membrane potential, by applying triple-barrelled ion-sensitive microelectrodes. As shown previously, ouabain induced a marked [Na(+)](i) increase, a [K(+)](i) decrease, and a membrane depolarization, and it also evoked an increase in [Cl(-)](i). The analysis of the data revealed a net uptake of NaCl, which quantitatively explained the ouabain-induced cell swelling. In the absence of extracellular Na(+) or Cl(-), NaCl uptake was excluded, and the cell volume remained unaffected. Likewise, NaCl uptake and, hence, cell swelling did not occur when the Na(+)-K(+) pump was inhibited by omitting bath K(+). Also, in K(+)-free solution, [Na(+)](i) increased and [K(+)](i) dropped, but [Cl(-)](i) slightly decreased, and after an initial, small membrane depolarization, the cells hyperpolarized for a prolonged period. It is concluded that the ouabain-induced NaCl uptake is caused by the depolarization of the plasma membrane, which augments the inwardly directed electrochemical Cl(-) gradient.

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“…Multi-barrel micropipettes were developed in the early eighties to monitor different ion levels simultaneously in clinical research, and they are employed in iontophoresis [12][13][14][15][16]. In some cases, they are effectively single ion selective electrodes whereas the other barrels are employed to deliver chemicals [17].…”

Section: Introductionmentioning

confidence: 99%

Improved potentiometric SECM imaging of galvanic corrosion reactions

et al. 2017

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The galvanic corrosion of Zn-Cu systems has been monitored in situ using scanning electrochemical microscopy in the potentiometric mode. Here, the initial studies performed using double-barrel microelectrodes for the simultaneous detection of local pH and Zn ion concentration changes in single lines scans, have been extended to the obtention of 2-D maps of Cu 2+ and Zn 2+ ions. The construction and calibration of a novel Cu 2+ ion selective microelectrode is reported. New scanning algorithms and data evaluation tools have been explored for faster acquisition rates than those limited by the time constants associated to potentiometric response.

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Multi-barrelled ion-selective microelectrodes as tools for the investigation of volume regulation mechanisms in invertebrate nerve cells under hyperosmotic conditions

Cited by 6 publications

References 19 publications

Swelling-activated chloride channels in leech Retzius neurons

Swelling-activated chloride channels in leech Retzius neurons

Ionic mechanism of ouabain-induced swelling of leech Retzius neurons

Improved potentiometric SECM imaging of galvanic corrosion reactions

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