Multi-analyte profiling of inflammatory mediators in COPD sputum – The effects of processing

Short-Term Variation of Lung Function and Airway Inflammation in Children and Adolescents with Bronchiolitis Obliterans

“…Sputum plaques are selected; however, according to the method, the soluble part of the sputum is not collected. By selecting the plaques, the variation of soluble cytokines might be greater than the variation of neutrophils [36]. Second researchers should be aware that the absolute concentration of selected inflammatory markers could be affected by DTT.…”

Obesity-Associated Metabolic Signatures Correlate to Clinical and Inflammatory Profiles of Asthma: A Pilot Study

“…Interleukin (IL)-1β, IL-4, IL-5, IL-6, IL-8, IL-13 and tumor necrosis factor (TNF)-α in sputum supernatant as local inflammation were detected by a Luminex-based MILLIPLEX ® MAP Human Cytokine/Chemokine Magnetic bead Panel Kit (EMD Millipore Corporation, Billerica, MA, USA), and were analyzed using the software of Milliplex Analyst 5.1 as it has been demonstrated that the Luminex-based xMAP ® panel can be used for multi-analyte profiling of sputum using the routinely applied method of sputum processing with dithiothreitol. 28 29 …”

Roflumilast combined with montelukast versus montelukast alone as add-on treatment in patients with moderate-to-severe asthma

“…20 Exploratory end points included total and differential WBC counts, a panel of 46 inflammatory mediators in serum measured by using the Human Inflammation Multi-Analyte Profile (MAP) detection panel (Myriad RBM kit; Rules-Based Medicine, Austin, Tex), symptoms, as-needed SABA use, ACQ-7 scores, and urinary leukotriene E 4 (LTE 4 ) levels. 21,22 In a post hoc responder analysis the number of patients who achieved an ACQ-7 score decrease of 0.5 or greater was also summarized. Additional exploratory end points included asthma exacerbations and dropouts caused by adverse events (AEs).…”

“…19 The analysis of inflammatory mediators in dithiothreitol-treated sputum supernatants was performed with the Human Inflammation MAP detection panel (Myriad RBM kit; Rules-Based Medicine; see the Methods section in this article's Online Repository). 22 Safety Safety monitoring included AEs, laboratory tests, measurement of body mass index (BMI) and vital signs, and electrocardiography. Safety assessment continued for 30 days after the active treatment period.…”