Multi-analyte analysis of non-vitamin K antagonist oral anticoagulants in human plasma using tandem mass spectrometry

Abstract

The method enables rapid and reliable simultaneous determination of NOAC concentrations in human plasma. It was successfully introduced into clinical practice; a case with rivaroxaban overdose is presented to exemplify the method's applicability.

Liquid chromatographic methods for the determination of direct oral anticoagulant drugs in biological samples: A critical review

“…Nevertheless, MEPS technique requires an additional and careful sorbent washing step after analytes elution in order to eliminate or reduce the carryover effect, enabling the reliable reuse of MEPS sorbent [29]. Taking were stable after three freeze-thaw cycles when stored at -80 ºC, and that edoxaban was stable after three freeze-thaw cycles when stored at -20 ºC [32,[41][42][43].…”

“…Considering the previously mentioned physicochemical properties of DOACs, it is not surprising that the published studies have commonly used solvents such as methanol and acetonitrile for the preparation of stock solutions [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43]. Some authors also used dimethyl sulfoxide (DMSO) and hydrochloric acid (HCl) 0.1 N [27,[35][36][37]43,44].…”

“…Chromatographic analysis of biological samples performed to determine the existing concentration of DOACs is mainly achieved by high resolution analytical techniques namely HPLC and UHPLC, although there is one example of turbulent flow liquid chromatography (Annex 4) [42]. This technique does not require sample preparation, reducing the analysis time and it is executed by using high flow rates and large particle size stationary phases [91].…”

Method Development and Validation of Selective and Highly Sensitive Method for Determination of Apixaban in Human Plasma Using Liquid Chromatography-Tandem Mass Spectrometry

“…Moreover, it requires minimal and simple sample preparation procedures. Though several methods are available for determination of apixaban in human plasma or serum on LC-MS/MS [3][4][5][6][7][8][9][10][11][12], they were applied either for PK study analysis or for TDM but not for both. Therefore, the objective of the current study was to develop and validate a highly sensitive method with the lowest possible sample volumes, so that the same method can be further used for TDM also apart from PK study analysis.…”

A Validated LC-MS/MS Method for the Estimation of Apixaban in Human Plasma

“…Approximately 50-55% of apixaban is eliminated in the feces and 20-25% is excreted in the urine. Earlier publications have described methods for determination of apixaban in biological matrix using liquid chromatography tandem mass spectrometry (LC-MS/MS) methods (Zhang et al, 2016;Baldelli et al, 2016;Noguez et al, 2016;Blaich et al, 2015;Schmitz et al, 2014;Gous et al, 2014;Delavenne et al, 2013). Many places UPLC have been used for separation.…”