Mulberry-Derived 1-Deoxynojirimycin Prevents Type 2 Diabetes Mellitus Progression via Modulation of Retinol-Binding Protein 4 and Haptoglobin

Fongsodsri, Kamonpan; Thaipitakwong, Thanchanit; Rujimongkon, Kitiya; Kanjanapruthipong, Tapanee; Ampawong, Sumate; Reamtong, Onrapak; Aramwit, Pornanong

doi:10.3390/nu14214538

Cited by 7 publications

(2 citation statements)

References 50 publications

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“…The liver and kidney mitochondria were extracted as described previously [ 9 , 10 , 11 , 12 , 13 , 14 ]. Each group’s liver and kidney were combined, homogenized in an extract buffer, and centrifuged at 1000× g and 4 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

High-Dose Primaquine Induces Proximal Tubular Degeneration and Ventricular Cardiomyopathy Linked to Host Cells Mitochondrial Dysregulation

Rabiablok

Hanboonkunupakarn

Tuentam

et al. 2023

Toxics

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Primaquine (PQ) is the only antimalarial medication used to eradicate many species of Plasmodium gametocytes and prevent relapse in vivax and ovale malarias. PQ metabolites induce oxidative stress and impair parasitic mitochondria, leading to protozoal growth retardation and death. Collateral damage is also presented in mammalian host cells, particularly erythrocytes, resulting in hemolysis and tissue destruction. However, the underlying mechanisms of these complications, particularly the mitochondria-mediated cell death of the host, are poorly understood. In the present study, toxicopathological studies were conducted on a rat model to determine the effect of PQ on affected tissues and mitochondrial toxicity. The results indicated that the LD50 for PQ is 200 mg/kg. A high dose of PQ induced hemolytic anemia, elevated a hepatic enzyme (SGPT), and induced proximal tubular degeneration, ventricular cardiomyopathy, and mitochondrial dysregulation. In addition, PQ induced the upregulation of apoptosis-related proteins Drp-1 and caspase-3, with a positive correlation, as well as the pro-apoptotic mitochondrial gene expression of Bax, reflecting the toxic effect of high doses of PQ on cellular damage and mitochondrial apoptosis in terms of hepatotoxicity, nephrotoxicity, and cardiotoxicity. Regarding the risk/benefit ratio of drug administration, our research provides caution for the use of PQ in the treatment of malaria based on its toxicopathological effects.

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Section: Methodsmentioning

confidence: 99%

High-Dose Primaquine Induces Proximal Tubular Degeneration and Ventricular Cardiomyopathy Linked to Host Cells Mitochondrial Dysregulation

Rabiablok

Hanboonkunupakarn

Tuentam

et al. 2023

Toxics

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“…Molecularly, much attention is currently being paid to the effects of active compounds in the context of normal glycemia. For prevention retinol-binding protein 4 and haptoglobinone downregulation in diabetic patients was shown as one of responsible mechanism [11].…”

Section: Introductionmentioning

confidence: 99%

Morus alba L. leaves modulate sweet (TAS1R) and bitter (TAS2R) taste in the studies on human receptors – is it a new perspectives of white mulberry leaves utilization in food production?

Przeor,

Jokiel

2023

Preprint

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From the nutritional point, the main direction for white mulberry (Morus alba L.) elements utilization so far is to create dietary supplements or functional foods for diabetics or over-weight patients. Leaves are widely known as a valuable source of bioactive compounds responsible for its antioxidant and antidiabetic effects, both in animals and humans. We found that processed leaves can be also considered as a bitter and/or sweet taste modulators – important aspect in designing of new functional foods. The aim of the study was to validate the inhibitory effect of Morus alba L. on the TAS2R3 and TAS2R13 bitter taste and TSA1R2/TSA1R3 receptors, and determine the changes that conditioning process caused in such receptors. Effect on the receptors was evaluated in specially transfected HEK293T cells, and the inhibition ratio was measured using the calcium release test. Moreover, the stability of phenolic acids and flavonols in the simulated intestinal in vitro digestion process was determined. Results showed that the tested Morus alba leaf extracts were rich in gallic, chlorogenic and caffeic acids together with rutin and quercetin 3-(6-malonyl)-glucoside, while the conditioning process positively affected on their amount. Mostly identified phenolics were reduced as a result of in vitro digestion. In taste receptors test, it was found that the phytochemicals from conditioned Morus alba leaf extract enhanced sweet taste, together with reduction of bitter taste receptors activity in some cases. To conclude, we found Morus alba, especially conditioned for 4 hours, seems to be valuable modulator of taste, what should be considered in future as a crucial reason for its new utilization.

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Morus alba L. Leaves (WML) Modulate Sweet (TAS1R) and Bitter (TAS2R) Taste in the Studies on Human Receptors – A New Perspective on the Utilization of White Mulberry Leaves in Food Production?

Przeor,

Jokiel

2023

Plant Foods Hum Nutr

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From the nutritional perspective, the main direction of the utilization of white mulberry (Morus alba L.) parts so far has been to produce dietary supplements or functional foods for individuals with diabetes or over-weight. Its leaves are widely known as a valuable source of bioactive compounds responsible for its antioxidant and antidiabetic effects, both in animals and humans. The authors found that processed leaves can also be investigated as potential bitter and/or sweet taste modulators—an important property of new functional foods. The study aimed to validate the inhibitory effect of Morus alba L. on the TAS2R3 and TAS2R13 bitter taste and TSA1R2/TSA1R3 receptors and determine the changes that the conditioning process caused in such receptors. The effect on the receptors was evaluated in specially transfected HEK293T cells, and the inhibition ratio was measured using the calcium release test. Moreover, the stability of phenolics in the simulated intestinal in vitro digestion process was determined. Results showed that the Morus alba leaf extracts were rich in gallic, chlorogenic and caffeic acids together with rutin and quercetin 3-(6-malonyl)-glucoside, while the conditioning process positively affected their amount. Most identified phenolics were reduced during in vitro digestion. In the taste receptors test, it was found that the phytochemicals from conditioned Morus alba leaf extract enhanced sweet taste, together with a reduction of bitter taste receptor activity in some cases. To conclude, the study has found that Morus alba, especially when conditioned for 4 h, seems to be a valuable modulator of taste, which should be considered in future research as a crucial reason for its new utilization.

show abstract

Mulberry-Derived 1-Deoxynojirimycin Prevents Type 2 Diabetes Mellitus Progression via Modulation of Retinol-Binding Protein 4 and Haptoglobin

Cited by 7 publications

References 50 publications

High-Dose Primaquine Induces Proximal Tubular Degeneration and Ventricular Cardiomyopathy Linked to Host Cells Mitochondrial Dysregulation

High-Dose Primaquine Induces Proximal Tubular Degeneration and Ventricular Cardiomyopathy Linked to Host Cells Mitochondrial Dysregulation

Morus alba L. leaves modulate sweet (TAS1R) and bitter (TAS2R) taste in the studies on human receptors – is it a new perspectives of white mulberry leaves utilization in food production?

Morus alba L. Leaves (WML) Modulate Sweet (TAS1R) and Bitter (TAS2R) Taste in the Studies on Human Receptors – A New Perspective on the Utilization of White Mulberry Leaves in Food Production?

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