Mulberry Anthocyanins Ameliorate DSS-Induced Ulcerative Colitis by Improving Intestinal Barrier Function and Modulating Gut Microbiota

Abstract

Mulberry has attracted wide attention due to its substantial nutritional values. This work first studied the protective effect of mulberry anthocyanins (MAS) on dextran sulfate sodium (DSS)-induced colitis. The mice experiment was designed as four groups including normal mice (Control), dextran sodium sulfate (DSS)-fed mice, and DSS plus 100 mg/kg·bw MAS-fed mice (LMAS-DSS) or DSS plus 200 mg/kg·bw MAS-fed mice (HMAS-DSS). Mice were given MAS by gavage for 1 week, and then DSS was added to the drinking water for 7 days. MAS was administered for a total of 17 days. The results showed that oral gavage of MAS reduced the disease activity index (DAI), prevented colon shortening, attenuated colon tissue damage and inflammatory response, suppressed colonic oxidative stress and restored the protein expression of intestinal tight junction (TJ) protein (ZO-1, occludin and claudin-3) in mice with DSS-induced colitis. In addition, analysis of 16S rRNA amplicon sequences showed that MAS reduced the DSS-induced intestinal microbiota dysbiosis, including a reduction in Escherichia-Shigella, an increase in Akkermansia, Muribaculaceae and Allobaculum. Collectively, MAS alleviates DSS-induced colitis by maintaining the intestinal barrier, modulating inflammatory cytokines, and improving the microbial community.

Effect of anthocyanins on gut health markers, Firmicutes-Bacteroidetes ratio and short-chain fatty acids: a systematic review via meta-analysis

“…This systematic review and meta-analysis demonstrated that dietary anthocyanin supplementation profoundly improves rodent models' gut health biomarkers (Fir/Bac and SCFAs). This finding was supported by studies carried out after cut off time limit of this studies [77][78][79][80][81] . According to certain research articles, the Fir/Bac ratio is a defining characteristic of obesity.…”

Efficient Therapy of Inflammatory Bowel Disease (IBD) with Highly Specific and Durable Targeted Ta2C Modified with Chondroitin Sulfate (TACS)

“…Importantly, Bacteroidetes had the highest relative abundance and occupied the main part of all flora, and had an important role in restoring the gut barrier and modulating inflammation. [ 50 ] The relative abundance of Bacteroidetes decreased in the IBD but increased significantly after TACS treatment. Compared with the healthy, the operational taxonomic unit (OTU) richness and α ‐diversity (Simpson and Shannon Index) were not changed in the other groups (Figure S43A–C, Supporting Information).…”

Virofree Associates with the Modulation of Gut Microbiomes and Alleviation of DSS-Induced IBD Symptoms in Mice

“…Recent studies have highlighted the potential protective role of genus Akkermansia against colitis. Specifically, Akkermansia muciniphila, a resident of the human gut, has been observed to have beneficial effects on the intestinal barrier function and inflammatory responses. , It is pertinent to mention that certain botanical extracts have shown to increase the abundance of this particular bacteria, fortifying the gut barrier and potentially ameliorating symptoms of IBD. , Although our study witnessed a rise in the Duncaniella genus, future research could delve into the potential synergies or complementary impacts of Virofree on other advantageous genera, such as Akkermansia.…”